pCMV-EGFP-P2A-TERT-3×FLAG-Puro

pCMV-EGFP-P2A-TERT-3×FLAG-Puro plasmid

PVT110056

pCMV-EGFP-P2A-TERT-3×FLAG-Puro plasmid Information

Plasmid name: pCMV-EGFP-P2A-TERT(human)-3×FLAG-Puro
Alias: NM_198253.3
Gene lenght: 3396
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-P2A-TERT-3×FLAG-Puro plasmid Description

pCMV-EGFP-P2A-TERT-3×FLAG-Puro It is a plasmid suitable for gene expression in mammalian cells.

pCMV-EGFP-P2A-TERT-3×FLAG-Puro plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 AAGCTTTCTA GAGAATTCga tatcGGATCC gccaccatgg tgagcaaggg cgaggagctg 961 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 1021 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1081 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc 1141 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1201 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1261 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1321 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 1381 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 1441 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1501 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1561 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1621 gggatcactc tcggcatgga cgagctgtac aagGCAACAA ACTTCTCTCT GCTGAAACAA 1681 GCCGGAGATG TCGAAGAGAA TCCTGGACCG ggtagtatgc cgcgcgctcc ccgctgccga 1741 gccgtgcgct ccctgctgcg cagccactac cgcgaggtgc tgccgctggc cacgttcgtg 1801 cggcgcctgg ggccccaggg ctggcggctg gtgcagcgcg gggacccggc ggctttccgc 1861 gcgctggtgg cccagtgcct ggtgtgcgtg ccctgggacg cacggccgcc ccccgccgcc 1921 ccctccttcc gccaggtgtc ctgcctgaag gagctggtgg cccgagtgct gcagaggctg 1981 tgcgagcgcg gcgcgaagaa cgtgctggcc ttcggcttcg cgctgctgga cggggcccgc 2041 gggggccccc ccgaggcctt caccaccagc gtgcgcagct acctgcccaa cacggtgacc 2101 gacgcactgc gggggagcgg ggcgtggggg ctgctgctgc gccgcgtggg cgacgacgtg 2161 ctggttcacc tgctggcacg ctgcgcgctc tttgtgctgg tggctcccag ctgcgcctac 2221 caggtgtgcg ggccgccgct gtaccagctc ggcgctgcca ctcaggcccg gcccccgcca 2281 cacgctagtg gaccccgaag gcgtctggga tgcgaacggg cctggaacca tagcgtcagg 2341 gaggccgggg tccccctggg cctgccagcc ccgggtgcga ggaggcgcgg gggcagtgcc 2401 agccgaagtc tgccgttgcc caagaggccc aggcgtggcg ctgcccctga gccggagcgg 2461 acgcccgttg ggcaggggtc ctgggcccac ccgggcagga cgcgtggacc gagtgaccgt 2521 ggtttctgtg tggtgtcacc tgccagaccc gccgaagaag ccacctcttt ggagggtgcg 2581 ctctctggca cgcgccactc ccacccatcc gtgggccgcc agcaccacgc gggcccccca 2641 tccacatcgc ggccaccacg tccctgggac acgccttgtc ccccggtgta cgccgagacc 2701 aagcacttcc tctactcctc aggcgacaag gagcagctgc ggccctcctt cctactcagc 2761 tctctgaggc ccagcctgac tggcgctcgg aggctcgtgg agaccatctt tctgggttcc 2821 aggccctgga tgccagggac tccccgcagg ttgccccgcc tgccccagcg ctactggcaa 2881 atgcggcccc tgtttctgga gctgcttggg aaccacgcgc agtgccccta cggggtgctc 2941 ctcaagacgc actgcccgct gcgagctgcg gtcaccccag cagccggtgt ctgtgcccgg 3001 gagaagcccc agggctctgt ggcggccccc gaggaggagg acacagaccc ccgtcgcctg 3061 gtgcagctgc tccgccagca cagcagcccc tggcaggtgt acggcttcgt gcgggcctgc 3121 ctgcgccggc tggtgccccc aggcctctgg ggctccaggc acaacgaacg ccgcttcctc 3181 aggaacacca agaagttcat ctccctgggg aagcatgcca agctctcgct gcaggagctg 3241 acgtggaaga tgagcgtgcg ggactgcgct tggctgcgca ggagcccagg ggttggctgt 3301 gttccggccg cagagcaccg tctgcgtgag gagatcctgg ccaagttcct gcactggctg 3361 atgagtgtgt acgtcgtcga gctgctcagg tctttctttt atgtcacgga gaccacgttt 3421 caaaagaaca ggctcttttt ctaccggaag agtgtctgga gcaagttgca aagcattgga 3481 atcagacagc acttgaagag ggtgcagctg cgggagctgt cggaagcaga ggtcaggcag 3541 catcgggaag ccaggcccgc cctgctgacg tccagactcc gcttcatccc caagcctgac 3601 gggctgcggc cgattgtgaa catggactac gtcgtgggag ccagaacgtt ccgcagagaa 3661 aagagggccg agcgtctcac ctcgagggtg aaggcactgt tcagcgtgct caactacgag 3721 cgggcgcggc gccccggcct cctgggcgcc tctgtgctgg gcctggacga tatccacagg 3781 gcctggcgca ccttcgtgct gcgtgtgcgg gcccaggacc cgccgcctga gctgtacttt 3841 gtcaaggtgg atgtgacggg cgcgtacgac accatccccc aggacaggct cacggaggtc 3901 atcgccagca tcatcaaacc ccagaacacg tactgcgtgc gtcggtatgc cgtggtccag 3961 aaggccgccc atgggcacgt ccgcaaggcc ttcaagagcc acgtctctac cttgacagac 4021 ctccagccgt acatgcgaca gttcgtggct cacctgcagg agaccagccc gctgagggat 4081 gccgtcgtca tcgagcagag ctcctccctg aatgaggcca gcagtggcct cttcgacgtc 4141 ttcctacgct tcatgtgcca ccacgccgtg cgcatcaggg gcaagtccta cgtccagtgc 4201 caggggatcc cgcagggctc catcctctcc acgctgctct gcagcctgtg ctacggcgac 4261 atggagaaca agctgtttgc ggggattcgg cgggacgggc tgctcctgcg tttggtggat 4321 gatttcttgt tggtgacacc tcacctcacc cacgcgaaaa ccttcctcag gaccctggtc 4381 cgaggtgtcc ctgagtatgg ctgcgtggtg aacttgcgga agacagtggt gaacttccct 4441 gtagaagacg aggccctggg tggcacggct tttgttcaga tgccggccca cggcctattc 4501 ccctggtgcg gcctgctgct ggatacccgg accctggagg tgcagagcga ctactccagc 4561 tatgcccgga cctccatcag agccagtctc accttcaacc gcggcttcaa ggctgggagg 4621 aacatgcgtc gcaaactctt tggggtcttg cggctgaagt gtcacagcct gtttctggat 4681 ttgcaggtga acagcctcca gacggtgtgc accaacatct acaagatcct cctgctgcag 4741 gcgtacaggt ttcacgcatg tgtgctgcag ctcccatttc atcagcaagt ttggaagaac 4801 cccacatttt tcctgcgcgt catctctgac acggcctccc tctgctactc catcctgaaa 4861 gccaagaacg cagggatgtc gctgggggcc aagggcgccg ccggccctct gccctccgag 4921 gccgtgcagt ggctgtgcca ccaagcattc ctgctcaagc tgactcgaca ccgtgtcacc 4981 tacgtgccac tcctggggtc actcaggaca gcccagacgc agctgagtcg gaagctcccg 5041 gggacgacgc tgactgccct ggaggccgca gccaacccgg cactgccctc agacttcaag 5101 accatcctgg acCTCGAGAC CGGTGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 5161 GACATCGATT ACAAGGATGA CGATGACAAG TGACCGGTaA GTTTAAACCG CTGATCAGCC 5221 TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG 5281 ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT 5341 TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG 5401 GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG 5461 GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC 5521 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 5581 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 5641 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 5701 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 5761 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 5821 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGGCCAT TTCGGCCTAT 5881 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT 5941 GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC 6001 ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA 6061 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC 6121 CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA 6181 TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT 6241 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCgggatg accgagtaca agcccacggt 6301 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc 6361 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga 6421 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga 6481 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt 6541 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca 6601 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt 6661 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt 6721 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct 6781 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc 6841 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaTTC GAAATGACCG ACCAAGCGAC 6901 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT 6961 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA 7021 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG 7081 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA 7141 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA 7201 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT 7261 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT 7321 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA 7381 ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC 7441 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA 7501 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 7561 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 7621 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 7681 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 7741 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 7801 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 7861 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 7921 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 7981 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 8041 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 8101 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA 8161 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 8221 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 8281 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 8341 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC 8401 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT 8461 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC 8521 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC 8581 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG 8641 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG 8701 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG 8761 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG 8821 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT 8881 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA 8941 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC 9001 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC 9061 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC 9121 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC 9181 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA 9241 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT 9301 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT 9361 C

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.