pEarley-35S-MCS-3×FLAG-Bar pla

pEarley-35S-MCS-3×FLAG-Bar plasmid

PVT109882

pEarley-35S-MCS-3×FLAG-Bar plasmid Information

Plasmid name: pEarley-35S-MCS-3×FLAG-Bar
Alias:
Gene lenght:
Host: Plant
Use(s): Gene expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Kan
Screening marker: Bar
Fluorescent labeling:
Promoter: 35S
Replicon: pVS1 oriV
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: 35S
Reverse primer:
Copy number: Low
Induction:

pEarley-35S-MCS-3×FLAG-Bar plasmid Description

pEarley-35S-MCS-3×FLAG-Bar Plasmid suitable for gene expression in plants.

pEarley-35S-MCS-3×FLAG-Bar plasmid Sequence

1 TGGCAGGATA TATTGTGGTG TAAACAAATT GACGCTTAGA CAACTTAATA ACACATTGCG 61 GACGTTTTTA ATGTACTGAA TTAACGCCGA ATTAATTCGA GCTCGGATCT GATAATTTAT 121 TTGAAAATTC ATAAGAAAAG CAAACGTTAC ATGAATTGAT GAAACAATAC AAAGACAGAT 181 AAAGCCACGC ACATTTAGGA TATTGGCCGA GATTACTGAA TATTGAGTAA GATCACGGAA 241 TTTCTGACAG GAGCATGTCT TCAATTCAGC CCAAATGGCA GTTGAAATAC TCAAACCGCC 301 CCATATGCAG GAGCGGATCA TTCATTGTTT GTTTGGTTGC CTTTGCCAAC ATGGGAGTCC 361 AAGATTCTGC AGTCAAATCT CGGTGACGGG CAGGACCGGA CGGGGCGGTA CCGGCAGGCT 421 GAAGTCCAGC TGCCAGAAAC CCACGTCATG CCAGTTCCCG TGCTTGAAGC CGGCCGCCCG 481 CAGCATGCCG CGGGGGGCAT ATCCGAGCGC CTCGTGCATG CGCACGCTCG GGTCGTTGGG 541 CAGCCCGATG ACAGCGACCA CGCTCTTGAA GCCCTGTGCC TCCAGGGACT TCAGCAGGTG 601 GGTGTAGAGC GTGGAGCCCA GTCCCGTCCG CTGGTGGCGG GGGGAGACGT ACACGGTCGA 661 CTCGGCCGTC CAGTCGTAGG CGTTGCGTGC CTTCCAGGGG CCCGCGTAGG CGATGCCGGC 721 GACCTCGCCG TCCACCTCGG CGACGAGCCA GGGATAGCGC TCCCGCAGAC GGACGAGGTC 781 GTCCGTCCAC TCCTGCGGTT CCTGCGGCTC GGTACGGAAG TTGACCGTGC TTGTCTCGAT 841 GTAGTGGTTG ACGATGGTGC AGACCGCCGG CATGTCCGCC TCGGTGGCAC GGCGGATGTC 901 GGCCGGGCGT CGTTCTGGGC TCATCGATTC GATTTGGTGT ATCGAGATTG GTTATGAAAT 961 TCAGATGCTA GTGTAATGTA TTGGTAATTT GGGAAGATAT AATAGGAAGC AAGGCTATTT 1021 ATCCATTTCT GAAAAGGCGA AATGGCGTCA CCGCGAGCGT CACGCGCATT CCGTTCTTGC 1081 TGTAAAGCGT TGTTTGGTAC ACTTTTGACT AGCGAGGCTT GGCGTGTCAG CGTATCTATT 1141 CAAAAGTCGT TAATGGCTGC GGATCAAGAA AAAGTTGGAA TAGAAACAGA ATACCCGCGA 1201 AATTCAGGCC CGGTTGCCAT GTCCTACACG CCGAAATAAA CGACCAAATT AGTAGAAAAA 1261 TAAAAACTGA CTCGGATACT TACGTCACGT CTTGCGCACT GATTTGAAAA ATCTCAGAAT 1321 TCCAATCCCA CAAAAATCTG AGCTTAACAG CACAGTTGCT CCTCTCAGAG CAGAATCGGG 1381 TATTCAACAC CCTCATATCA ACTACTACGT TGTGTATAAC GGTCCACATG CCGGTATATA 1441 CGATGACTGG GGTTGTACAA AGGCGGCAAC AAACGGCGTT CCCGGAGTTG CACACAAGAA 1501 ATTTGCCACT ATTACAGAGG CAAGAGCAGC AGCTGACGCG TACACAACAA GTCAGCAAAC 1561 AGACAGGTTG AACTTCATCC CCAAAGGAGA AGCTCAACTC AAGCCCAAGA GCTTTGCTAA 1621 GGCCCTAACA AGCCCACCAA AGCAAAAAGC CCACTGGCTC ACGCTAGGAA CCAAAAGGCC 1681 CAGCAGTGAT CCAGCCCCAA AAGAGATCTC CTTTGCCCCG GAGATTACAA TGGACGATTT 1741 CCTCTATCTT TACGATCTAG GAAGGAAGTT CGAAGGTGAA GGTGACGACA CTATGTTCAC 1801 CACTGATAAT GAGAAGGTTA GCCTCTTCAA TTTCAGAAAG AATGCTGACC CACAGATGGT 1861 TAGAGAGGCC TACGCAGCAG GTCTCATCAA GACGATCTAC CCGAGTAACA ATCTCCAGGA 1921 GATCAAATAC CTTCCCAAGA AGGTTAAAGA TGCAGTCAAA AGATTCAGGA CTAATTGCAT 1981 CAAGAACACA GAGAAAGACA TATTTCTCAA GATCAGAAGT ACTATTCCAG TATGGACGAT 2041 TCAAGGCTTG CTTCATAAAC CAAGGCAAGT AATAGAGATT GGAGTCTCTA AAAAGGTAGT 2101 TCCTACTGAA TCTAAGGCCA TGCATGGAGT CTAAGATTCA AATCGAGGAT CTAACAGAAC 2161 TCGCCGTGAA GACTGGCGAA CAGTTCATAC AGAGTCTTTT ACGACTCAAT GACAAGAAGA 2221 AAATCTTCGT CAACATGGTG GAGCACGACA CTCTGGTCTA CTCCAAAAAT GTCAAAGATA 2281 CAGTCTCAGA AGACCAAAGG GCTATTGAGA CTTTTCAACA AAGGATAATT TCGGGAAACC 2341 TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCGA AAGGACAGTA GAAAAGGAAG 2401 GTGGCTCCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCATTCAA GATCTCTCTG 2461 CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG 2521 TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGACAT CTCCACTGAC GTAAGGGATG 2581 ACGCACAATC CCACTATCCT TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT 2641 TGGAGAGGAC ACGCTCGAGC CATGGGGATC CGACTACAAA GACCATGACG GTGATTATAA 2701 AGATCATGAC ATCGATTACA AGGATGACGA TGACAAGtaa TCTAGAGAGT TAATTAAGAC 2761 CCGGGACTAG TCCCTAGAGT CCTGCTTTAA TGAGATATGC GAGACGCCTA TGATCGCATG 2821 ATATTTGCTT TCAATTCTGT TGTGCACGTT GTAAAAAACC TGAGCATGTG TAGCTCAGAT 2881 CCTTACCGCC GGTTTCGGTT CATTCTAATG AATATATCAC CCGTTACTAT CGTATTTTTA 2941 TGAATAATAT TCTCCGTTCA ATTTACTGAT TGTACCCTAC TACTTATATG TACAATATTA 3001 AAATGAAAAC AATATATTGT GCTGAATAGG TTTATAGCGA CATCTATGAT AGAGCGCCAC 3061 AATAACAAAC AATTGCGTTT TATTATTACA AATCCAATTT TAAAAAAAGC GGCAGAACCG 3121 GTCAAACCTA AAAGACTGAT TACATAAATC TTATTCAAAT TTCAAAAGTG CCCCAGGGGC 3181 TAGTATCTAC GACACACCGA GCGGCGAACT AATAACGCTC ACTGAAGGGA ACTCCGGTTC 3241 CCCGCCGGCG CGCATGGGTG AGATTCCTTG AAGTTGAGTA TTGGCCGTCC GCTCTACCGA 3301 AAGTTACGGG CACCATTCAA CCCGGTCCAG CACGGCGGCC GGGTAACCGA CTTGCTGCCC 3361 CGAGAATTAT GCAGCATTTT TTTGGTGTAT GTGGGCCCCA AATGAAGTGC AGGTCAAACC 3421 TTGACAGTGA CGACAAATCG TTGGGCGGGT CCAGGGCGAA TTTTGCGACA ACATGTCGAG 3481 GCTCAGCAGG ACCTGCAGGC ATGCAAGCTT GGCACTGGCC GTCGTTTTAC AACGTCGTGA 3541 CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG 3601 CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA 3661 TGGCGAATGC TAGAGCAGCT TGAGCTTGGA TCAGATTGTC GTTTCCCGCC TTCAGTTTAA 3721 ACTATCAGTG TTTGACAGGA TATATTGGCG GGTAAACCTA AGAGAAAAGA GCGTTTATTA 3781 GAATAACGGA TATTTAAAAG GGCGTGAAAA GGTTTATCCG TTCGTCCATT TGTATGTGCA 3841 TGCCAACCAC AGGGTTCCCC TCGGGATCAA AGTACTTTGA TCCAACCCCT CCGCTGCTAT 3901 AGTGCAGTCG GCTTCTGACG TTCAGTGCAG CCGTCTTCTG AAAACGACAT GTCGCACAAG 3961 TCCTAAGTTA CGCGACAGGC TGCCGCCCTG CCCTTTTCCT GGCGTTTTCT TGTCGCGTGT 4021 TTTAGTCGCA TAAAGTAGAA TACTTGCGAC TAGAACCGGA GACATTACGC CATGAACAAG 4081 AGCGCCGCCG CTGGCCTGCT GGGCTATGCC CGCGTCAGCA CCGACGACCA GGACTTGACC 4141 AACCAACGGG CCGAACTGCA CGCGGCCGGC TGCACCAAGC TGTTTTCCGA GAAGATCACC 4201 GGCACCAGGC GCGACCGCCC GGAGCTGGCC AGGATGCTTG ACCACCTACG CCCTGGCGAC 4261 GTTGTGACAG TGACCAGGCT AGACCGCCTG GCCCGCAGCA CCCGCGACCT ACTGGACATT 4321 GCCGAGCGCA TCCAGGAGGC CGGCGCGGGC CTGCGTAGCC TGGCAGAGCC GTGGGCCGAC 4381 ACCACCACGC CGGCCGGCCG CATGGTGTTG ACCGTGTTCG CCGGCATTGC CGAGTTCGAG 4441 CGTTCCCTAA TCATCGACCG CACCCGGAGC GGGCGCGAGG CCGCCAAGGC CCGAGGCGTG 4501 AAGTTTGGCC CCCGCCCTAC CCTCACCCCG GCACAGATCG CGCACGCCCG CGAGCTGATC 4561 GACCAGGAAG GCCGCACCGT GAAAGAGGCG GCTGCACTGC TTGGCGTGCA TCGCTCGACC 4621 CTGTACCGCG CACTTGAGCG CAGCGAGGAA GTGACGCCCA CCGAGGCCAG GCGGCGCGGT 4681 GCCTTCCGTG AGGACGCATT GACCGAGGCC GACGCCCTGG CGGCCGCCGA GAATGAACGC 4741 CAAGAGGAAC AAGCATGAAA CCGCACCAGG ACGGCCAGGA CGAACCGTTT TTCATTACCG 4801 AAGAGATCGA GGCGGAGATG ATCGCGGCCG GGTACGTGTT CGAGCCGCCC GCGCACGTCT 4861 CAACCGTGCG GCTGCATGAA ATCCTGGCCG GTTTGTCTGA TGCCAAGCTG GCGGCCTGGC 4921 CGGCCAGCTT GGCCGCTGAA GAAACCGAGC GCCGCCGTCT AAAAAGGTGA TGTGTATTTG 4981 AGTAAAACAG CTTGCGTCAT GCGGTCGCTG CGTATATGAT GCGATGAGTA AATAAACAAA 5041 TACGCAAGGG GAACGCATGA AGGTTATCGC TGTACTTAAC CAGAAAGGCG GGTCAGGCAA 5101 GACGACCATC GCAACCCATC TAGCCCGCGC CCTGCAACTC GCCGGGGCCG ATGTTCTGTT 5161 AGTCGATTCC GATCCCCAGG GCAGTGCCCG CGATTGGGCG GCCGTGCGGG AAGATCAACC 5221 GCTAACCGTT GTCGGCATCG ACCGCCCGAC GATTGACCGC GACGTGAAGG CCATCGGCCG 5281 GCGCGACTTC GTAGTGATCG ACGGAGCGCC CCAGGCGGCG GACTTGGCTG TGTCCGCGAT 5341 CAAGGCAGCC GACTTCGTGC TGATTCCGGT GCAGCCAAGC CCTTACGACA TATGGGCCAC 5401 CGCCGACCTG GTGGAGCTGG TTAAGCAGCG CATTGAGGTC ACGGATGGAA GGCTACAAGC 5461 GGCCTTTGTC GTGTCGCGGG CGATCAAAGG CACGCGCATC GGCGGTGAGG TTGCCGAGGC 5521 GCTGGCCGGG TACGAGCTGC CCATTCTTGA GTCCCGTATC ACGCAGCGCG TGAGCTACCC 5581 AGGCACTGCC GCCGCCGGCA CAACCGTTCT TGAATCAGAA CCCGAGGGCG ACGCTGCCCG 5641 CGAGGTCCAG GCGCTGGCCG CTGAAATTAA ATCAAAACTC ATTTGAGTTA ATGAGGTAAA 5701 GAGAAAATGA GCAAAAGCAC AAACACGCTA AGTGCCGGCC GTCCGAGCGC ACGCAGCAGC 5761 AAGGCTGCAA CGTTGGCCAG CCTGGCAGAC ACGCCAGCCA TGAAGCGGGT CAACTTTCAG 5821 TTGCCGGCGG AGGATCACAC CAAGCTGAAG ATGTACGCGG TACGCCAAGG CAAGACCATT 5881 ACCGAGCTGC TATCTGAATA CATCGCGCAG CTACCAGAGT AAATGAGCAA ATGAATAAAT 5941 GAGTAGATGA ATTTTAGCGG CTAAAGGAGG CGGCATGGAA AATCAAGAAC AACCAGGCAC 6001 CGACGCCGTG GAATGCCCCA TGTGTGGAGG AACGGGCGGT TGGCCAGGCG TAAGCGGCTG 6061 GGTTGTCTGC CGGCCCTGCA ATGGCACTGG AACCCCCAAG CCCGAGGAAT CGGCGTGAGC 6121 GGTCGCAAAC CATCCGGCCC GGTACAAATC GGCGCGGCGC TGGGTGATGA CCTGGTGGAG 6181 AAGTTGAAGG CCGCGCAGGC CGCCCAGCGG CAACGCATCG AGGCAGAAGC ACGCCCCGGT 6241 GAATCGTGGC AAGCGGCCGC TGATCGAATC CGCAAAGAAT CCCGGCAACC GCCGGCAGCC 6301 GGTGCGCCGT CGATTAGGAA GCCGCCCAAG GGCGACGAGC AACCAGATTT TTTCGTTCCG 6361 ATGCTCTATG ACGTGGGCAC CCGCGATAGT CGCAGCATCA TGGACGTGGC CGTTTTCCGT 6421 CTGTCGAAGC GTGACCGACG AGCTGGCGAG GTGATCCGCT ACGAGCTTCC AGACGGGCAC 6481 GTAGAGGTTT CCGCAGGGCC GGCCGGCATG GCCAGTGTGT GGGATTACGA CCTGGTACTG 6541 ATGGCGGTTT CCCATCTAAC CGAATCCATG AACCGATACC GGGAAGGGAA GGGAGACAAG 6601 CCCGGCCGCG TGTTCCGTCC ACACGTTGCG GACGTACTCA AGTTCTGCCG GCGAGCCGAT 6661 GGCGGAAAGC AGAAAGACGA CCTGGTAGAA ACCTGCATTC GGTTAAACAC CACGCACGTT 6721 GCCATGCAGC GTACGAAGAA GGCCAAGAAC GGCCGCCTGG TGACGGTATC CGAGGGTGAA 6781 GCCTTGATTA GCCGCTACAA GATCGTAAAG AGCGAAACCG GGCGGCCGGA GTACATCGAG 6841 ATCGAGCTAG CTGATTGGAT GTACCGCGAG ATCACAGAAG GCAAGAACCC GGACGTGCTG 6901 ACGGTTCACC CCGATTACTT TTTGATCGAT CCCGGCATCG GCCGTTTTCT CTACCGCCTG 6961 GCACGCCGCG CCGCAGGCAA GGCAGAAGCC AGATGGTTGT TCAAGACGAT CTACGAACGC 7021 AGTGGCAGCG CCGGAGAGTT CAAGAAGTTC TGTTTCACCG TGCGCAAGCT GATCGGGTCA 7081 AATGACCTGC CGGAGTACGA TTTGAAGGAG GAGGCGGGGC AGGCTGGCCC GATCCTAGTC 7141 ATGCGCTACC GCAACCTGAT CGAGGGCGAA GCATCCGCCG GTTCCTAATG TACGGAGCAG 7201 ATGCTAGGGC AAATTGCCCT AGCAGGGGAA AAAGGTCGAA AAGGTCTCTT TCCTGTGGAT 7261 AGCACGTACA TTGGGAACCC AAAGCCGTAC ATTGGGAACC GGAACCCGTA CATTGGGAAC 7321 CCAAAGCCGT ACATTGGGAA CCGGTCACAC ATGTAAGTGA CTGATATAAA AGAGAAAAAA 7381 GGCGATTTTT CCGCCTAAAA CTCTTTAAAA CTTATTAAAA CTCTTAAAAC CCGCCTGGCC 7441 TGTGCATAAC TGTCTGGCCA GCGCACAGCC GAAGAGCTGC AAAAAGCGCC TACCCTTCGG 7501 TCGCTGCGCT CCCTACGCCC CGCCGCTTCG CGTCGGCCTA TCGCGGCCGC TGGCCGCTCA 7561 AAAATGGCTG GCCTACGGCC AGGCAATCTA CCAGGGCGCG GACAAGCCGC GCCGTCGCCA 7621 CTCGACCGCC GGCGCCCACA TCAAGGCACC CTGCCTCGCG CGTTTCGGTG ATGACGGTGA 7681 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG 7741 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT 7801 GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG 7861 ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA 7921 TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 7981 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 8041 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 8101 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 8161 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 8221 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 8281 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG 8341 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 8401 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 8461 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 8521 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 8581 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 8641 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 8701 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 8761 CGTTAAGGGA TTTTGGTCAT GCATTCTAGG TACTAAAACA ATTCATCCAG TAAAATATAA 8821 TATTTTATTT TCTCCCAATC AGGCTTGATC CCCAGTAAGT CAAAAAATAG CTCGACATAC 8881 TGTTCTTCCC CGATATCCTC CCTGATCGAC CGGACGCAGA AGGCAATGTC ATACCACTTG 8941 TCCGCCCTGC CGCTTCTCCC AAGATCAATA AAGCCACTTA CTTTGCCATC TTTCACAAAG 9001 ATGTTGCTGT CTCCCAGGTC GCCGTGGGAA AAGACAAGTT CCTCTTCGGG CTTTTCCGTC 9061 TTTAAAAAAT CATACAGCTC GCGCGGATCT TTAAATGGAG TGTCTTCTTC CCAGTTTTCG 9121 CAATCCACAT CGGCCAGATC GTTATTCAGT AAGTAATCCA ATTCGGCTAA GCGGCTGTCT 9181 AAGCTATTCG TATAGGGACA ATCCGATATG TCGATGGAGT GAAAGAGCCT GATGCACTCC 9241 GCATACAGCT CGATAATCTT TTCAGGGCTT TGTTCATCTT CATACTCTTC CGAGCAAAGG 9301 ACGCCATCGG CCTCACTCAT GAGCAGATTG CTCCAGCCAT CATGCCGTTC AAAGTGCAGG 9361 ACCTTTGGAA CAGGCAGCTT TCCTTCCAGC CATAGCATCA TGTCCTTTTC CCGTTCCACA 9421 TCATAGGTGG TCCCTTTATA CCGGCTGTCC GTCATTTTTA AATATAGGTT TTCATTTTCT 9481 CCCACCAGCT TATATACCTT AGCAGGAGAC ATTCCTTCCG TATCTTTTAC GCAGCGGTAT 9541 TTTTCGATCA GTTTTTTCAA TTCCGGTGAT ATTCTCATTT TAGCCATTTA TTATTTCCTT 9601 CCTCTTTTCT ACAGTATTTA AAGATACCCC AAGAAGCTAA TTATAACAAG ACGAACTCCA 9661 ATTCACTGTT CCTTGCATTC TAAAACCTTA AATACCAGAA AACAGCTTTT TCAAAGTTGT 9721 TTTCAAAGTT GGCGTATAAC ATAGTATCGA CGGAGCCGAT TTTGAAACCG CGGTGATCAC 9781 AGGCAGCAAC GCTCTGTCAT CGTTACAATC AACATGCTAC CCTCCGCGAG ATCATCCGTG 9841 TTTCAAACCC GGCAGCTTAG TTGCCGTTCT TCCGAATAGC ATCGGTAACA TGAGCAAAGT 9901 CTGCCGCCTT ACAACGGCTC TCCCGCTGAC GCCGTCCCGG ACTGATGGGC TGCCTGTATC 9961 GAGTGGTGAT TTTGTGCCGA GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.