pSK+KanaRpsL
Catalog No. PVT10537
Packing 2ug
pSK+KanaRpsL Information
Function Other source plasmids
Replicator: pUC ori, F1 ori
Plasmid classification: gene library plasmid; other gene plasmids; other source plasmids.
Plasmid size: 4678bp
Plasmid label: Kana-RpsL
Prokaryotic resistance: ampicillin Amp (100 u g/ml)
Cloned strains of Escherichia coli, DH5 A and other Escherichia coli
Culture conditions: 37 centigrade, aerobic, LB
Expression host: mammalian cells such as 293T
Culture conditions: 37 C, 5%CO2
Induction mode: no induction
pSK+KanaRpsL Description
PSK+KanaRpsL has a complete KanaRpsL gene template.
pSK+KanaRpsL Sequence
LOCUS Exported 4678 bp ds-DNA circular SYN 14-AUG-2017
DEFINITION synthetic circular DNA
KEYWORDS pSK+KanaRpsL.dna
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4678)
TITLE Direct Submission
JOURNAL Exported Aug 14, 2017
FEATURES Location/Qualifiers
source 1..4678
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1031..1405
/codon_start=1
/label=RpsL
/translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKP
NSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGALDC
SGVKDRKQARSKYGVKRPKA"
CDS 1446..2240
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter complement(2487..2505)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
rep_origin complement(2928..3516)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3687..4547)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4548..4652)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
661 gccccccctc gaggtcgacg gtatcgataa gcttcacgct gccgcaagca ctcagggcgc
721 aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc
781 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
841 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
901 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
961 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctaaccag
1021 gagctattta atggcaacag ttaaccagct ggtacgcaaa ccacgtgctc gcaaagttgc
1081 gaaaagcaac gtgcctgcgc tggaagcatg cccgcaaaaa cgtggcgtat gtactcgtgt
1141 atatactacc actcctaaaa aaccgaactc cgcgctgcgt aaagtatgcc gtgttcgtct
1201 gactaacggt ttcgaagtga cttcctacat cggtggtgaa ggtcacaacc tgcaggagca
1261 ctccgtgatc ctgatccgtg gcggtcgtgt taaagacctc ccgggtgttc gttaccacac
1321 cgtacgtggt gcgcttgact gctccggcgt taaagaccgt aagcaggctc gttccaagta
1381 tggcgtgaag cgtcctaagg cttaatggta gatctgatca agagacagga tgacggtcgt
1441 ttcgcatgct tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
1501 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
1561 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
1621 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
1681 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
1741 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
1801 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
1861 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
1921 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
1981 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
2041 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
2101 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
2161 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
2221 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
2281 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
2341 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
2401 cttcgcccac cccgggctcg atcccctcgg ggggatccac tagttctaga gcggccgcca
2461 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattccgag cttggcgtaa
2521 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
2581 cgagccggaa gcataaagtg taaa
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