pCMV-3×FLAG-DNM1L-Puro plasmid

pCMV-3×FLAG-DNM1L-Puro plasmid

PVT106207

pCMV-3×FLAG-DNM1L-Puro plasmid Information

Plasmid name: pCMV-3×FLAG-DNM1L(human)-Puro
Alias: NM_012062.5;DLP1; DRP1; DVLP; DYMPLE; EMPF; EMPF1;HDYNIV; OPA5
Gene lenght: 2211
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-DNM1L-Puro plasmid Description

pCMV-3×FLAG-DNM1L-Puro is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-DNM1L-Puro plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAg ccaccatgga ctacaaagac 901 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagCTTatg 961 gaggcgctaa ttcctgtcat aaacaagctc caggacgtct tcaacacggt gggcgccgac 1021 atcatccagc tgcctcaaat cgtcgtagtg ggaacgcaga gcagcggaaa gagctcagtg 1081 ctagaaagcc tggtggggag ggacctgctt cccagaggta ctggaattgt cacccggaga 1141 cctctcattc tgcaactggt ccatgtttca caagaagata aacggaaaac aacaggagaa 1201 gaaaatgggg tggaagcaga agaatggggt aaatttcttc acaccaaaaa taagctttac 1261 acggattttg atgaaattcg acaagaaatt gaaaatgaaa cagaaagaat ttcaggaaat 1321 aataagggag taagccctga accaattcat cttaagattt tttcacccaa cgttgtcaat 1381 ttgacacttg tggatttgcc aggaatgacc aaggtgcctg taggtgatca acctaaggat 1441 attgagcttc aaatcagaga gctcattctt cggttcatca gtaatcctaa ttccattatc 1501 ctcgctgtca ctgctgctaa tacagatatg gcaacatcag aggcacttaa aatttcaaga 1561 gaggtagatc cagatggtcg cagaacccta gctgtaatca ctaaacttga tctcatggat 1621 gcgggtactg atgccatgga tgtattgatg ggaagggtta ttccagtcaa acttggaata 1681 attggagtag ttaacaggag ccagctagat attaacaaca agaagagtgt aactgattca 1741 atccgtgatg agtatgcttt tcttcaaaag aaatatccat ctctggccaa tagaaatgga 1801 acaaagtatc ttgctaggac tctaaacagg ttactgatgc atcacatcag agattgttta 1861 ccagagttga aaacaagaat aaatgttcta gctgctcagt atcagtctct tctaaatagc 1921 tacggtgaac ccgtggatga taaaagtgct actttactcc aacttattac caaatttgcc 1981 acagaatatt gtaacactat tgaaggaact gcaaaatata ttgaaacttc ggagctatgc 2041 ggtggtgcta gaatttgtta tattttccat gagacttttg ggcgaacctt agaatctgtt 2101 gatccacttg gtggccttaa cactattgac attttgactg ccattagaaa tgctactggt 2161 cctcgtcctg ctttatttgt gcctgaggtt tcatttgagt tactggtgaa gcggcaaatc 2221 aaacgtctag aagagcccag cctccgctgt gtggaactgg ttcatgagga aatgcaaagg 2281 atcattcagc actgtagcaa ttacagtaca caggaattgt tacgatttcc taaacttcat 2341 gatgccatag ttgaagtggt gacttgtctt cttcgtaaaa ggttgcctgt tacaaatgaa 2401 atggtccata acttagtggc aattgaactg gcttatatca acacaaaaca tccagacttt 2461 gctgatgctt gtgggctaat gaacaataat atagaggaac aaaggagaaa caggctagcc 2521 agagaattac cttcagctgt atcacgagac aagtcttcta aagttccaag tgctttggca 2581 cctgcctccc aggagccctc ccccgctgct tctgctgagg ctgatggcaa gttaattcag 2641 gacagcagaa gagaaactaa aaatgttgca tctggaggtg gtggggttgg agatggtgtt 2701 caagaaccaa ccacaggcaa ctggagagga atgctgaaaa cttcaaaagc tgaagagtta 2761 ttagcagaag aaaaatcaaa acccattcca attatgccag ccagtccaca aaaaggtcat 2821 gccgtgaacc tgctagatgt gccagttcct gttgcacgaa aactatctgc tcgggaacag 2881 cgagattgtg aggttattga acgactcatt aaatcatatt ttctcattgt cagaaagaat 2941 attcaagaca gtgtgccaaa ggcagtaatg cattttttgg ttaatcatgt gaaagacact 3001 cttcagagtg agctagtagg ccagctgtat aaatcatcct tattggatga tcttctgaca 3061 gaatctgagg acatggcaca gcgcaggaaa gaagcagctg atatgctaaa ggcattacaa 3121 ggagccagtc aaattattgc tgaaatccgg gagactcatc tttggtgaGA ATTCTGCAGA 3181 TATCCAGCAC AGTGGCGGCC GCTCGAGTCT AGAGGGCCCG TTTAAACCCG CTGATCAGCC 3241 TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG 3301 ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT 3361 TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG 3421 GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG 3481 GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC 3541 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 3601 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 3661 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 3721 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 3781 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 3841 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 3901 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT 3961 GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC 4021 ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA 4081 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC 4141 CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA 4201 TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT 4261 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT 4321 CTGATCAAGA GACAGGATGA GGATCGTTTC GCatgaccga gtacaagccc acggtgcgcc 4381 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact 4441 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc 4501 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 4561 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 4621 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 4681 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 4741 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 4801 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 4861 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 4921 cctggtgcat gacccgcaag cccggtgcct gaGCGGGACT CTGGGGTTCG AAATGACCGA 4981 CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG 5041 GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT 5101 CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA 5161 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG 5221 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG 5281 CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC 5341 ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA 5401 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA 5461 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC 5521 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC 5581 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 5641 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 5701 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 5761 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 5821 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 5881 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 5941 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 6001 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 6061 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 6121 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 6181 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT 6241 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 6301 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 6361 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 6421 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 6481 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 6541 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC 6601 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 6661 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 6721 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT 6781 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 6841 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 6901 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 6961 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 7021 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA 7081 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 7141 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 7201 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 7261 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 7321 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 7381 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 7441 ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.