pCMV-E6(HPV18)-Neo plasmid

pCMV-E6(HPV18)-Neo plasmid

PVT104061

pCMV-E6(HPV18)-Neo plasmid Information

Plasmid name: pCMV-E6(HPV18)-Neo
Alias:
Gene lenght: 474
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human tumor Virus18
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-E6(HPV18)-Neo plasmid Description

pCMV-E6(HPV18)-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-E6(HPV18)-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccgccaccAT GGCGCGCTTT GAGGATCCAA CACGGCGACC CTACAAGCTA 961 CCTGATCTGT GCACGGAACT GAACACTTCA CTGCAAGACA TAGAAATAAC CTGTGTATAT 1021 TGCAAGACAG TATTGGAACT TACAGAGGTA TTTGAATTTG CATTTAAAGA TTTATTTGTG 1081 GTGTATAGAG ACAGTATACC CCATGCTGCA TGCCATAAAT GTATAGATTT TTATTCTAGA 1141 ATTAGAGAAT TAAGACATTA TTCAGACTCT GTGTATGGAG ACACATTGGA AAAACTAACT 1201 AACACTGGGT TATACAATTT ATTAATAAGG TGCCTGCGGT GCCAGAAACC GTTGAATCCA 1261 GCAGAAAAAC TTAGACACCT TAATGAAAAA CGACGATTTC ACAACATAGC TGGGCACTAT 1321 AGAGGCCAGT GCCATTCGTG CTGCAACCGA GCACGACAGG AACGACTCCA ACGACGCAGA 1381 GAAACACAAG TATAAgaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca 1441 tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac 1501 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct 1561 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct 1621 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg 1681 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag 1741 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc 1801 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 1861 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 1921 tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 1981 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 2041 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 2101 ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt 2161 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag 2221 ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct 2281 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 2341 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 2401 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 2461 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 2521 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 2581 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 2641 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 2701 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 2761 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 2821 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 2881 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 2941 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 3001 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 3061 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 3121 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 3181 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 3241 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 3301 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 3361 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 3421 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc 3481 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 3541 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat 3601 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 3661 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc 3721 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 3781 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 3841 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 3901 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 3961 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 4021 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 4081 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 4141 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 4201 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 4261 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 4321 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc 4381 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 4441 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 4501 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 4561 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 4621 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 4681 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 4741 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 4801 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 4861 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 4921 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 4981 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 5041 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 5101 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 5161 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 5221 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 5281 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 5341 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 5401 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 5461 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 5521 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 5581 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 5641 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 5701 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 5761 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 5821 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 5881 ccccgaaaag tgccacctga cgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.