pLV3-CMV-SLC5A6-Puro plasmid

pLV3-CMV-SLC5A6-Puro plasmid

PVT111572

pLV3-CMV-SLC5A6-Puro plasmid Information

Plasmid name: pLV3-CMV-SLC5A6(human)-Puro
Alias: NM_021095.4;COMNB; hSMVT; NERIB; SMVT; SMVTD
Gene lenght: 1908
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SLC5A6-Puro plasmid Description

pLV3-CMV-SLC5A6-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SLC5A6-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCgccac catgagtgta ggggtgagca cctcagcccc tctttcccca 2341 acctcgggca caagcgtggg catgtctacc ttctccatca tggactatgt ggtgttcgtc 2401 ctgctgctgg ttctctctct tgccattggg ctctaccatg cttgtcgtgg ctggggccgg 2461 catactgttg gtgagctgct gatggcggac cgcaaaatgg gctgccttcc ggtggcactg 2521 tccctgctgg ccaccttcca gtcagccgtg gccatcctgg gtgtgccgtc agagatctac 2581 cgatttggga cccaatattg gttcctgggc tgctgctact ttctggggct gctgatacct 2641 gcacacatct tcatccccgt tttctaccgc ctgcatctca ccagtgccta tgagtacctg 2701 gagcttcgat tcaataaaac tgtgcgagtg tgtggaactg tgaccttcat ctttcagatg 2761 gtgatctaca tgggagttgt gctctatgct ccgtcattgg ctctcaatgc agtgactggc 2821 tttgatctgt ggctgtccgt gctggccctg ggcattgtct gtaccgtcta tacagctctg 2881 ggtgggctga aggccgtcat ctggacagat gtgttccaga cactggtcat gttcctcggg 2941 cagctggcag ttatcattgt ggggtcagcc aaggtgggcg gcttggggcg tgtgtgggcc 3001 gtggcttccc agcacggccg catctctggg tttgagctgg atccagaccc ctttgtgcgg 3061 cacaccttct ggaccttggc cttcgggggt gtcttcatga tgctctcctt atacggggtg 3121 aaccaggctc aggtgcagcg gtacctcagt tcccgcacgg agaaggctgc tgtgctctcc 3181 tgttatgcag tgttcccctt ccagcaggtg tccctctgcg tgggctgcct cattggcctg 3241 gtcatgttcg cgtattacca ggagtatccc atgagcattc agcaggctca ggcagcccca 3301 gaccagttcg tcctgtactt tgtgatggat ctcctgaagg gcctgccagg cctgccaggg 3361 ctcttcattg cctgcctctt cagcggctct ctcagcacta tatcctctgc ttttaattca 3421 ttggcaactg ttacgatgga agacctgatt cgaccttggt tccctgagtt ctctgaagcc 3481 cgggccatca tgctttccag aggccttgcc tttggctatg ggctgctttg tctaggaatg 3541 gcctatattt cctcccagat gggacctgtg ctgcaggcag caatcagcat ctttggcatg 3601 gttgggggac cgctgctggg actcttctgc cttggaatgt tctttccatg tgctaaccct 3661 cctggtgctg ttgtgggcct gttggctggg ctcgtcatgg ccttctggat tggcatcggg 3721 agcatcgtga ccagcatggg ctccagcatg ccaccctctc cctctaatgg gtccagcttc 3781 tccctgccca ccaatctaac cgttgccact gtgaccacac tgatgccctt gactaccttc 3841 tccaagccca cagggctgca gcggttctat tccttgtctt acttatggta cagtgctcac 3901 aactccacca cagtgattgt ggtgggcctg attgtcagtc tactcactgg gagaatgcga 3961 ggccggtccc tgaaccctgc aaccatttac ccagtgttgc caaagctcct gtccctcctt 4021 ccgttgtcct gtcagaagcg gctccactgc aggagctacg gccaggacca cctcgacact 4081 ggcctgtttc ctgagaagcc gaggaatggt gtgctggggg acagcagaga caaggaggcc 4141 atggccctgg atggcacagc ctatcagggg agcagctcca cctgcatcct ccaggagacc 4201 tccctgtgaG GATCCGCGGC CGCGAAGGAT CTGCGATCGC TCCGGTGCCC GTCAGTGGGC 4261 AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACGGG 4321 TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT 4381 TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT 4441 TCGCAACGGG TTTGCCGCCA GAACACAGCT GAAGCTTCGA GGGGCTCGCA TCTCTCCTTC 4501 ACGCGCCCGC CGCCCTACCT GAGGCCGCCA TCCACGCCGG TTGAGTCGCG TTCTGCCGCC 4561 TCCCGCCTGT GGTGCCTCCT GAACTGCGTC CGCCGTCTAG GTAAGTTTAA AGCTCAGGTC 4621 GAGACCGGGC CTTTGTCCGG CGCTCCCTTG GAGCCTACCT AGACTCAGCC GGCTCTCCAC 4681 GCTTTGCCTG ACCCTGCTTG CTCAACTCTA CGTCTTTGTT TCGTTTTCTG TTCTGCGCCG 4741 TTACAGATCC AAGCTGTGAC CGGCGCCTAC GCTAGATGAC CGAGTACAAG CCCACGGTGC 4801 GCCTCGCCAC CCGCGACGAC GTCCCCCGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG 4861 ACTACCCCGC CACGCGCCAC ACCGTCGACC CGGACCGCCA CATCGAGCGG GTCACCGAGC 4921 TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG 4981 ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG 5041 CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA 5101 TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG 5161 GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG 5221 AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC 5281 CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC 5341 GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGAGTCGA CAATCAACCT CTGGATTACA 5401 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 5461 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 5521 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 5581 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 5641 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 5701 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 5761 TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA 5821 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 5881 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA 5941 GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGTACCTT TAAGACCAAT GACTTACAAG 6001 GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC 6061 TCCCAACGAA AATAAGATCT GCTTTTTGCT TGTACTGGGT CTCTCTGGTT AGACCAGATC 6121 TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG 6181 CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC 6241 CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTA GTAGTTCATG TCATCTTATT 6301 ATTCAGTATT TATAACTTGC AAAGAAATGA ATATCAGAGA GTGAGAGGAA CTTGTTTATT 6361 GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT 6421 TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG 6481 CTCTAGCTAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC 6541 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT 6601 AGTGAGGAGG CTTTTTTGGA GGCCTAGACT TTTGCAGAGA CGGCCCAAAT TCGTAATCAT 6661 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 6721 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 6781 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 6841 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 6901 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 6961 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 7021 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 7081 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 7141 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 7201 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 7261 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 7321 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 7381 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 7441 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 7501 GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 7561 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 7621 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 7681 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 7741 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 7801 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 7861 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 7921 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 7981 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 8041 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 8101 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 8161 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 8221 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 8281 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 8341 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 8401 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 8461 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 8521 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 8581 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 8641 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 8701 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 8761 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT 8821 AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC 8881 GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG 8941 TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG 9001 GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG 9061 TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC 9121 GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC 9181 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG 9241 GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGC CAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.