pLV3-CMV-WNT11-8×His-CopGFP-Pu

pLV3-CMV-WNT11-8×His-CopGFP-Puro plasmid

PVT103759

pLV3-CMV-WNT11-8×His-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-WNT11(human)-8×His-CopGFP-Puro
Alias: NM_004626.3;HWNT11
Gene lenght: 1062
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-WNT11-8×His-CopGFP-Puro plasmid Description

pLV3-CMV-WNT11-8×His-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-WNT11-8×His-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga gggcgcggcc gcaggtctgc 2341 gaggcgctgc tcttcgccct ggcgctccag accggcgtgt gctatggcat caagtggctg 2401 gcgctgtcca agacaccatc ggccctggca ctgaaccaga cgcaacactg caagcagctg 2461 gagggtctgg tgtctgcaca ggtgcagctg tgccgcagca acctggagct catgcacacg 2521 gtggtgcacg ccgcccgcga ggtcatgaag gcctgtcgcc gggcctttgc cgacatgcgc 2581 tggaactgct cctccattga gctcgccccc aactatttgc ttgacctgga gagagggacc 2641 cgggagtcgg ccttcgtgta tgcgctgtcg gccgccgcca tcagccacgc catcgcccgg 2701 gcctgcacct ccggcgacct gcccggctgc tcctgcggcc ccgtcccagg tgagccaccc 2761 gggcccggga accgctgggg aggatgtgcg gacaacctca gctacgggct cctcatgggg 2821 gccaagtttt ccgatgctcc tatgaaggtg aaaaaaacag gatcccaagc caataaactg 2881 atgcgtctac acaacagtga agtggggaga caggctctgc gcgcctctct ggaaatgaag 2941 tgtaagtgcc atggggtgtc tggctcctgc tccatccgca cctgctggaa ggggctgcag 3001 gagctgcagg atgtggctgc tgacctcaag acccgatacc tgtcggccac caaggtagtg 3061 caccgaccca tgggcacccg caagcacctg gtgcccaagg acctggatat ccggcctgtg 3121 aaggactcgg aactcgtcta tctgcagagc tcacctgact tctgcatgaa gaatgagaag 3181 gtgggctccc acgggacaca agacaggcag tgcaacaaga catccaacgg aagcgacagc 3241 tgcgacctta tgtgctgcgg gcgtggctac aacccctaca cagaccgcgt ggtcgagcgg 3301 tgccactgta agtaccactg gtgctgctac gtcacctgcc gcaggtgtga gcgtaccgtg 3361 gagcgctatg tctgcaaggg atccCACCAT CACCATCACC ATCACCATta gcggccgcGa 3421 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 3481 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta 3541 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 3601 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 3661 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc 3721 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact 3781 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc 3841 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa 3901 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg 3961 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag 4021 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc 4081 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc 4141 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc 4201 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc 4261 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc 4321 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc 4381 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat 4441 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc 4501 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac 4561 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc 4621 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag 4681 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc 4741 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac 4801 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 4861 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 4921 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 4981 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 5041 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 5101 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 5161 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 5221 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 5281 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 5341 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg 5401 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 5461 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 5521 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 5581 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 5641 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 5701 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 5761 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 5821 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 5881 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct 5941 cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact 6001 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 6061 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac 6121 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 6181 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 6241 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 6301 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg 6361 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 6421 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 6481 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 6541 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 6601 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt 6661 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 6721 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 6781 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 6841 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 6901 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 6961 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 7021 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 7081 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 7141 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 7201 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 7261 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 7321 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 7381 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 7441 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 7501 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 7561 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 7621 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 7681 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 7741 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 7801 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 7861 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 7921 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 7981 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 8041 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 8101 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 8161 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 8221 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 8281 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 8341 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 8401 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 8461 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 8521 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 8581 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 8641 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 8701 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 8761 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 8821 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 8881 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 8941 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 9001 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 9061 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 9121 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 9181 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 9241 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.