PiggyBac-NAT plasmid

PiggyBac-NAT plasmid

PVT99003

PiggyBac-NAT plasmid Information

Plasmid name: PiggyBac-NAT
Alias: -
Gene lenght:
Host: Mammalian cells
Use(s): Protein Expression,Transposition
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

PiggyBac-NAT plasmid Description

PiggyBac-NAT is a plasmid suitable for protein expression and transposition in mammalian cells.

PiggyBac-NAT plasmid Sequence

1 ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT 61 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA 121 CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC 181 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC 241 CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG 301 GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA 361 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC 421 GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT 481 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG 541 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT 601 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC 661 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT 721 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC 781 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC 841 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT 901 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG 961 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC 1021 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA 1081 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG 1141 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA 1201 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA 1261 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG 1321 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG 1381 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG 1441 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG 1501 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC 1561 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA 1621 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG 1681 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT 1741 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA 1801 GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG 1861 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG 1921 CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA 1981 ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCGC 2041 GCCCGCCGGG TAACTCACGG GGTATCCATG TCCATTTCTG CGGCATCCAG CCAGGATACC 2101 CGTCCTCGCT GACGTAATAT CCCAGCGCCG CACCGCTGTC ATTAATCTGC ACACCGGCAC 2161 GGCAGTTCCG GCTGTCGCCG GTATTGTTCG GGTTGCTGAT GCGCTTCGGG CTGACCATCC 2221 GGAACTGTGT CCGGAAAAGC CGCGACGAAC TGGTATCCCA GGTGGCCTGA ACGAACAGTT 2281 CACCGTTAAA GGCGTGCATG GCCACACCTT CCCGAATCAT CATGGTAAAC GTGCGTTTTC 2341 GCTCAACGTC AATGCAGCAG CAGTCATCCT CGGCAAACTC TTTCCATGCC GCTTCAACCT 2401 CGCGGGAAAA GGCACGGGCT TCTTCCTCCC CGATGCCCAG ATAGCGCCAG CTTGGGCGAT 2461 GACTGAGCCG GAAAAAAGAC CCGACGATAT GATCCTGATG CAGCTAGATT AACCCTAGAA 2521 AGATAGTCTG CGTAAAATTG ACGCATGCAT TCTTGAAATA TTGCTCTCTC TTTCTAAATA 2581 GCGCGAATCC GTCGCTGTGC ATTTAGGACA TCTCAGTCGC CGCTTGGAGC TCCCGTGAGG 2641 CGTGCTTGTC AATGCGGTAA GTGTCACTGA TTTTGAACTA TAACGACCGC GTGAGTCAAA 2701 ATGACGCATG ATTATCTTTT ACGTGACTTT TAAGATTTAA CTCATACGAT AATTATATTG 2761 TTATTTCATG TTCTACTTAC GTGATAACTT ATTATATATA TATTTTCTTG TTATAGATAT 2821 Cagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg 2881 gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc 2941 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa 3001 cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa aaagccatac 3061 caatgggccc taaaaaagga atccagtcaa ttccggggct aaacctggct gccactgttt 3121 ctttagggac ttcgttcctg tgaggacagg aaaccctgca acctggttga ccagagcaag 3181 ggatataaga acccagtttg ggaacactgc tgtttaaata ttaaacaggg atgtcttgtc 3241 ccccggaagg gagaccagat agcttctgat agactaattg acatcatttg agtcgtaaaa 3301 cgacggccag tgaattgtaa tacgactcac tatagggcga attggagctc caccgcggtg 3361 gcggccgctc tagaactagt ggatcgaagt tcctatactt tctagagaat aggaacttcg 3421 tgcgcttttg aaccacgtaa agtgctgtaa accagctcta ttcaatgagg taaatgataa 3481 agtttataag agaccaaaaa attcaaaaaa aaaattgaaa aattatattg gtatttatat 3541 gaaacaatag ttcagttcaa agcgctagct tttctcatct caactaaccg gtattaggtg 3601 ggtatttatt taatatggga acaagaaatg tgacattaat gttcatgttt ttattttcat 3661 tatttattct atatggtttg ggacttgatg ttatgtctta attcatagag atccgcttgc 3721 ctcgtccccg ccgggtcacc cggccagcca ccataccata gcttcaaaat gtttctactc 3781 cttttttact cttccagatt ttctcggact ccgcgcatcg ccgtaccact tcaaaacacc 3841 caagcacagc atactaaatt tcccctcttt cttcctctag ggtgtcgtta attacccgta 3901 ctaaaggttt ggaaaagaaa aaagagaccg cctcgtttct ttttcttcgt cgaaaaaggc 3961 aataaaaatt tttatcacgt ttctttttct tgaaaatttt tttttgattt ttttctcttt 4021 cgatgacctc ccattgatat ttaagttaat aaacggtctt caatttctca agtttcagtt 4081 tcatttttct tgttctatta caactttttt tacttcttgc tcattagaaa gaaagcatag 4141 caatctaatc taagttttgg atctaaaatg ggcaccactc ttgacgacac ggcttaccga 4201 taccgcacca gtgtcccggg ggacgccgag gccatcgagg cactggatgg gtccttcacc 4261 accgacaccg tcttccgcgt caccgccacc ggggacggct tcaccctgcg ggaggtgccg 4321 gtggacccgc ccctgaccaa ggtgttcccc gacgacgaat cggacgacga atcggacgac 4381 ggggaggacg gcgaccctga ctcccggacg ttcgtcgcgt acggggacga cggcgacctg 4441 gcgggcttcg tggtcgtctc gtactccggc tggaaccgcc ggctgaccgt cgaggacatc 4501 gaggtcgccc cggagcaccg ggggcacggg gtcgggcgcg cgttgatggg gctcgcgacg 4561 gagttcgccc gcgagctggg cgccgggcac ctctggctgg aggtcaccaa cgtcaacgca 4621 ccggcgatcc acgcgtaccg gcggatgggg ttcaccctct gcggcctgga caccgccctg 4681 tacgacggca ccgcctcgga cggcgagcag gcgctctaca tgagcatgcc ctgcccctaa 4741 gtcgagtcat gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc 4801 taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag 4861 ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac 4921 gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga 4981 aggctttaat ttgcctgtcg attcgatact aacgccgcca tccagtgtcg atcgacgaag 5041 ttcctatact ttctagagaa taggaacttc gtcctcgagg gggggcccgg taccagcttt 5101 tgttcccttt agtgagggtt aattccgagc ttggcgtaat catggtcata gctgtttcct 5161 gaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa 5221 gtcccataag gtcatgtact gggcataatA CTAGTAGGCC TTGGAGGCCT TTTCCCCGTA 5281 TCCCCCCAGG TGTCTGCAGG CTCAAAGAGC AGCGAGAAGC GTTCAGAGGA AAGCGATCCC 5341 GTGCCACCTT CCCCGTGCCC GGGCTGTCCC CGCACGCTGC CGGCTCGGGG ATGCGGGGGG 5401 AGCGCCGGAC CGGAGCGGAG CCCCGGGCGG CTCGCTGCTG CCCCCTAGCG GGGGAGGGAC 5461 GTAATTACAT CCCTGGGGGC TTTGGGGGGG GGCTGTCCCT CATAAAAGTT TTGTTACTTT 5521 ATAGAAGAAA TTTTGAGTTT TTGTTTTTTT TTAATAAATA AATAAACATA AATAAATTGT 5581 TTGTTGAATT TATTATTAGT ATGTAAGTGT AAATATAATA AAACTTAATA TCTATTCAAA 5641 TTAATAAATA AACCTCGATA TACAGACCGA TAAAACACAT GCGTCAATTT TACGCATGAT 5701 TATCTTTAAC GTACGTCACA ATATGATTAT CTTTCTAGGG TTAATCTAGC TGCGTGTTCT 5761 GCAGCGTGTC GAGCATCTTC ATCTGCTCCA TCACGCTGTA AAACACATTT GCACCGCGAG 5821 TCTGCCCGTC CTCCACGGGT TCAAAAACGT GAATGAACGA GGCGCGCTCA CTGGCCGTCG 5881 TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC 5941 ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC 6001 AGTTGCGCAG CCTGAATGGC GAATGGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 6061 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 6121 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 6181 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 6241 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 6301 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 6361 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 6421 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA 6481 TTTAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT 6541 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG 6601 AAAAAGGAAG AGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.