pLV3-CMV-WIF1-3×FLAG-CopGFP-Pu

pLV3-CMV-WIF1-3×FLAG-CopGFP-Puro plasmid

PVT97013

pLV3-CMV-WIF1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-WIF1(human)-3×FLAG-CopGFP-Puro
Alias: NM_007191.5;WIF-1
Gene lenght: 1137bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-WIF1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-WIF1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-WIF1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cccggaggag cgccttccct 2341 gccgccgcgc tctggctctg gagcatcctc ctgtgcctgc tggcactgcg ggcggaggcc 2401 gggccgccgc aggaggagag cctgtaccta tggatcgatg ctcaccaggc aagagtactc 2461 ataggatttg aagaagatat cctgattgtt tcagagggga aaatggcacc ttttacacat 2521 gatttcagaa aagcacaaca gagaatgcca gctattcctg tcaatatcca ttccatgaat 2581 tttacctggc aagctgcagg gcaggcagaa tacttctatg aattcctgtc cttgcgctcc 2641 ctggataaag gcatcatggc agatccaacc gtcaatgtcc ctctgctggg aacagtgcct 2701 cacaaggcat cagttgttca agttggtttc ccatgtcttg gaaaacagga tggggtggca 2761 gcatttgaag tggatgtgat tgttatgaat tctgaaggca acaccattct ccaaacacct 2821 caaaatgcta tcttctttaa aacatgtcaa caagctgagt gcccaggcgg gtgccgaaat 2881 ggaggctttt gtaatgaaag acgcatctgc gagtgtcctg atgggttcca cggacctcac 2941 tgtgagaaag ccctttgtac cccacgatgt atgaatggtg gactttgtgt gactcctggt 3001 ttctgcatct gcccacctgg attctatgga gtgaactgtg acaaagcaaa ctgctcaacc 3061 acctgcttta atggagggac ctgtttctac cctggaaaat gtatttgccc tccaggacta 3121 gagggagagc agtgtgaaat cagcaaatgc ccacaaccct gtcgaaatgg aggtaaatgc 3181 attggtaaaa gcaaatgtaa gtgttccaaa ggttaccagg gagacctctg ttcaaagcct 3241 gtctgcgagc ctggctgtgg tgcacatgga acctgccatg aacccaacaa atgccaatgt 3301 caagaaggtt ggcatggaag acactgcaat aaaaggtacg aagccagcct catacatgcc 3361 ctgaggccag caggcgccca gctcaggcag cacacgcctt cacttaaaaa ggccgaggag 3421 cggcgggatc cacctgaatc caattacatc tggggatccG ACTACAAAGA CCATGACGGT 3481 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGtagcg gccgcGaagg 3541 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 3601 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 3661 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 3721 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 3781 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 3841 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 3901 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 3961 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 4021 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 4081 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 4141 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 4201 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 4261 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 4321 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 4381 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 4441 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 4501 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 4561 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 4621 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 4681 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 4741 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 4801 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 4861 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag 4921 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 4981 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 5041 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 5101 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 5161 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 5221 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 5281 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 5341 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 5401 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 5461 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat 5521 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 5581 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 5641 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 5701 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 5761 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 5821 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 5881 tccgtggtgt tgtcggggaa ATCATCGTCC TTTCCTtggc tgctcgcctg tgttgccacc 5941 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 6001 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctTcgcct tcgccctcag 6061 acgagtcgga tctccctttg gGccgcctcc ccgcctggta cctttaagac caatgactta 6121 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 6181 tcactcccaa cgaaGataag atctgctttt tgcttgtact gggtctctct ggttagacca 6241 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 6301 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 6361 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 6421 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 6481 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 6541 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 6601 ctggctctag ctaTCCCGCC CCTAACTCCG CCCAtcccgc ccctaactcc gcccagttcc 6661 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 6721 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 6781 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 6841 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 6901 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 6961 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 7021 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 7081 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 7141 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 7201 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 7261 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 7321 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 7381 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 7441 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 7501 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 7561 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 7621 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 7681 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 7741 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 7801 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 7861 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 7921 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 7981 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 8041 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 8101 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 8161 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 8221 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 8281 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 8341 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 8401 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 8461 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 8521 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 8581 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 8641 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 8701 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 8761 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 8821 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 8881 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 8941 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 9001 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 9061 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 9121 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 9181 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 9241 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 9301 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 9361 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 9421 caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.