pLV3-CMV-MCS2-3×FLAG-CopGFP-Pu

pLV3-CMV-MCS2-3×FLAG-CopGFP-Puro plasmid

PVT94626

pLV3-CMV-MCS2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-MCS2-3×FLAG-CopGFP-Puro
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MCS2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-MCS2-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-MCS2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gtcgacGATA TCcccgggac cggtCTCGAG 2341 ggatccGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 2401 GACGATGACA AGtagcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca 2461 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 2521 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 2581 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 2641 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 2701 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 2761 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 2821 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 2881 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 2941 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc 3001 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc 3061 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag 3121 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc 3181 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac 3241 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg 3301 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc 3361 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg 3421 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc 3481 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc 3541 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag 3601 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc 3661 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc 3721 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag 3781 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 3841 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 3901 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 3961 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 4021 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 4081 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 4141 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 4201 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 4261 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 4321 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 4381 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 4441 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 4501 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 4561 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 4621 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 4681 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 4741 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaATC ATCGTCCTTT 4801 CCTtggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 4861 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 4921 cttccgcgtc tTcgccttcg ccctcagacg agtcggatct ccctttggGc cgcctccccg 4981 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 5041 agaaaagggg ggactggaag ggctaattca ctcccaacga aGataagatc tgctttttgc 5101 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 5161 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 5221 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 5281 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 5341 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 5401 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 5461 caaactcatc aatgtatctt atcatgtctg gctctagcta TCCCGCCCCT AACTCCGCCC 5521 Atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 5581 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 5641 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt 5701 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 5761 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 5821 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 5881 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 5941 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 6001 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 6061 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 6121 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 6181 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 6241 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 6301 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 6361 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 6421 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 6481 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 6541 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 6601 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 6661 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 6721 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 6781 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 6841 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 6901 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 6961 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 7021 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 7081 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 7141 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 7201 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 7261 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 7321 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 7381 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 7441 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 7501 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 7561 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 7621 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 7681 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 7741 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 7801 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 7861 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 7921 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 7981 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 8041 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 8101 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 8161 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 8221 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 8281 acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.