pENTR-CMV-Crls1-FLAG-EGFP plas

pENTR-CMV-Crls1-FLAG-EGFP plasmid

PVT96780

pENTR-CMV-Crls1-FLAG-EGFP plasmid Information

Plasmid name: pENTR-CMV-Crls1(rat)-FLAG-EGFP
Alias: NM_001014258.1 ;RGD1311037
Gene lenght: 906bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pENTR-CMV-Crls1-FLAG-EGFP plasmid Description

pENTR-CMV-Crls1-FLAG-EGFP is a plasmid suitable for protein expression in mammalian cells.

pENTR-CMV-Crls1-FLAG-EGFP plasmid Sequence

1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCC GCGGCCGCCG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA 721 CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT 781 CAATAATGAC GTATGTTCCC ATAGTAACGT CAATAGGGAC TTTCCATTGA CGTCAATGGG 841 TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA 901 CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA 961 CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG 1021 TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC 1081 CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT 1141 TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT 1201 GGGAGGTCTA TATAAGCAGA GCTCtctaga ctcgagaccg gtCTTAAGGC TAGCGATATC 1261 GGATCCgcca ccatgctggc ttggcgcgtg gcacgcggcg cgtgggggtc ccttcgcgtg 1321 gccgtccggc ctccaggggc gcggctcggc aggggcggct cccgcagggc cctgctacca 1381 cccgcggcct gctgcctggg ctgcctggcc gagcgctggc ggctgcgtcc ggccgcgttc 1441 gccttgcggc tgccaggcac cagcccgcgg acccactgct ccggcgccgg gaaggcagcc 1501 ccggagcccg cagccggagg agatgccgcc gcgcaggccc ctagtgcccg gtgggtccgg 1561 gcgagcgcaa ccagctcgta tgaaaatcca tggacaatcc caaatttatt gtcaatgaca 1621 agaattggcc tggccccggt gttgggctat ctgattcttg aagaagattt taatgttgca 1681 ctgggtgttt ttgctttagc tgggctaacg gatttgttgg atggatttat tgctcgaaac 1741 tgggccaatc aaaaatcagc tttgggaagt gctcttgatc cacttgctga taaggttctt 1801 atcagcatct tatatattag cctgacctat gcagatctta ttccagtccc actcacttac 1861 atgataattt caagagatgt aatgttgatc gctgctgtgt tttatgtcag ataccgaact 1921 ctgccaacac cgcgaacact agctaagtac ttcaatcctt gctatgccac tgctaggtta 1981 aaaccaacat tcatcagcaa ggtcaataca gcagtccagt taattttggt ggcagcttct 2041 ctggcagctc cagttttcaa ttatgctgac agcatttatc ttcagatact atggtgttgt 2101 acagcattca ctacagctgc gtccgcatac agttattatc actatggtcg gaaaactgtt 2161 caggtgataa agggcaaaAC TAGTGATTAC AAGGACGACG ATGACAAGGT CGACgaattc 2221 GGCAGTGGAG AGGGCAGAGG AAGTCTGCTA ACATGCGGTG ACGTCGAGGA GAATCCTGGC 2281 CCAGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC 2341 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC 2401 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC 2461 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG 2521 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC 2581 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG 2641 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC 2701 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC 2761 GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC 2821 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC 2881 TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC 2941 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGtaa 3001 AGATCTACGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG 3061 CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT 3121 AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG 3181 GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC 3241 AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC 3301 CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT 3361 AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT 3421 ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG 3481 TCCTTCTGAT TTTGTAGGTA ACCACGTGCG GACGTTTAAA CGGCGCGCCG ACCCAGCTTT 3541 CTTGTACAAA GTTGGCATTA TAAGAAAGCA TTGCTTATCA ATTTGTTGCA ACGAACAGGT 3601 CACTATCAGT CAAAATAAAA TCATTATTTG CCATCCAGCT GATATCCCCT ATAGTGAGTC 3661 GTATTACATG GTCATAGCTG TTTCCTGGCA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG 3721 TTACATTGCA CAAGATAAAA ATATATCATC ATGAACAATA AAACTGTCTG CTTACATAAA 3781 CAGTAATACA AGGGGTGTTA TGAGCCATAT TCAACGGGAA ACGTCGAGGC CGCGATTAAA 3841 TTCCAACATG GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG TCGGGCAATC 3901 AGGTGCGACA ATCTATCGCT TGTATGGGAA GCCCGATGCG CCAGAGTTGT TTCTGAAACA 3961 TGGCAAAGGT AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA ACTGGCTGAC 4021 GGAATTTATG CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG ATGCATGGTT 4081 ACTCACCACT GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT ATCCTGATTC 4141 AGGTGAAAAT ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT CGATTCCTGT 4201 TTGTAATTGT CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC AATCACGAAT 4261 GAATAACGGT TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT GGCCTGTTGA 4321 ACAAGTCTGG AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG TCGTCACTCA 4381 TGGTGATTTC TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG GTTGTATTGA 4441 TGTTGGACGA GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT GGAACTGCCT 4501 CGGTGAGTTT TCTCCTTCAT TACAGAAACG GCTTTTTCAA AAATATGGTA TTGATAATCC 4561 TGATATGAAT AAATTGCAGT TTCATTTGAT GCTCGATGAG TTTTTCTAAT CAGAATTGGT 4621 TAATTGGTTG TAACACTGGC AGAGCATTAC GCTGACTTGA CGGGACGGCG CAAGCTCATG 4681 ACCAAAATCC CTTAACGTGA GTTACGCGTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 4741 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 4801 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 4861 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA 4921 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 4981 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 5041 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 5101 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC 5161 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 5221 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 5281 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 5341 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 5401 CATGTT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.