pKO3-Km plasmid

pKO3-Km plasmid

PVT96986

pKO3-Km plasmid Information

Plasmid name: pKO3-Km
Alias: -
Gene lenght:
Host: E.coli
Use(s): Gene editing
Fragment type: CRISPR
Species: Empty Vector
Prokaryotic resistance: Kan
Screening marker: SacB
Fluorescent labeling:
Promoter: Non
Replicon: pSC101
Growth Strain(s): EPI300
Growth Temperature: 30°C
Vector backbone:
Forward primer: KanR-F3
Reverse primer:
Copy number: Low
Induction:

pKO3-Km plasmid Description

pKO3-Km is a plasmid suitable for gene editing in Escherichia coli.

pKO3-Km plasmid Sequence

1 CTTAAAACCC TAAAGGCTTA AGTAGCACCC TCGCAAGCTC GGGCAAATCG CTGAATATTC 61 CTTTTGTCTC CGACCATCAG GCACCTGAGT CGCTGTCTTT TTCGTGACAT TCAGTTCGCT 121 GCGCTCACGG CTCTGGCAGT GAATGGGGGT AAATGGCACT ACAGGCGCCT TTTATGGATT 181 CATGCAAGGA AACTACCCAT AATACAAGAA AAGCCCGTCA CGGGCTTCTC AGGGCGTTTT 241 ATGGCGGGTC TGCTATGTGG TGCTATCTGA CTTTTTGCTG TTCAGCAGTT CCTGCCCTCT 301 GATTTTCCAG TCTGACCACT TCGGATTATC CCGTGACAGG TCATTCAGAC TGGCTAATGC 361 ACCCAGTAAG GCAGCGGTAT CATCAACAGG CTTACCCGTC TTACTGTCnG GATCGACGCT 421 CTCCCTTATG CGACTCCTGC ATCCCTTTCG TCTTCGAATA AATACCTGTG ACGGAAGATC 481 ACTTCGCAGA ATAAATAAAT CCTGGTGTCC CTGTTGATAC CGGGAAGCCC TGGGCCAACT 541 TTTGGCGAAA ATGAGACGTt tagaaaaact catcgagcat caaatgaaac tgcaatttat 601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagattatgc atttctttcc 841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1201 ctttgccatg tttcagaaac aactctggcg catggggctt cccatacaag cgatagattg 1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1321 tgttggaatt taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcataacac 1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat 1441 cttgtgcaat gtaacatcag agattttgag acGCTACGCC TGAATAAGTG ATAATAAGCG 1501 GATGAATGGC AGAAATTCGA AAGCAAATTC GACCCGGTCG TCGGTTCAGG GCAGGGTCGT 1561 TAAATAGCCG CTTATGTCTA TTGCTGGTTT ANTCGGTACC CGGGgatcgc ggccgcggac 1621 cggatcctct agagcggccg cGATCCTCTA GAGTCGACCG GNGAATGGCG AATGGGACGC 1681 GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC 1741 ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT 1801 CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC 1861 TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC 1921 GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT 1981 CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG 2041 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC 2101 GAATTTTAAC AAAATATTAA CGCTTACAAT TTAGGTGGCA CTTTTCGGGG AAATGTGCGC 2161 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATNNCANGA 2221 TCCTTTTTAA CCCATCACAT ATACCTGCCG TTCACTATTA TTTAGTGAAA TGAGATATTA 2281 TGATATTTTC TGAATTGTGA TTAAAAAGGC AACTTTATGC CCATGCAACA GAAACTATAA 2341 AAAATACAGA GAATGAAAAG AAACAGATAG ATTTTTTAGT TCTTTAGGCC CGTAGTCTGC 2401 AAATCCTTTT ATGATTTTCT ATCAAACAAA AGAGGAAAAT AGACCAGTTG CAATCCAAAC 2461 GAGAGTCTAA TAGAATGAGG TCGAAAAGTA AATCGCGCGG GTTTGTTACT GATAAAGCAG 2521 GCAAGACCTA AAATGTGTAA AGGGCAAAGT GTATACTTTG GCGTCACCCC TTACATATTT 2581 TAGGTCTTTT TTTATTGTGC GTAACTAACT TGCCATCTTC AAACAGGAGG GCTGGAAGAA 2641 GCAGACCGCT AACACAGTAC ATAAAAAAGG AGACATGAAC GATGAACATC AAAAAGTTTG 2701 CAAAACAAGC AACAGTATTA ACCTTTACTA CCGCACTGCT GGCAGGAGGC GCAACTCAAG 2761 CGTTTGCGAA AGAAACGAAC CAAAAGCCAT ATAAGGAAAC ATACGGCATT TCCCATATTA 2821 CACGCCATGA TATGCTGCAA ATCCCTGAAC AGCAAAAAAA TGAAAAATAT CAAGTTCCTG 2881 AATTCGATTC GTCCACAATT AAAAATATCT CTTCTGCAAA AGGCCTGGAC GTTTGGGACA 2941 GCTGGCCATT ACAAAACGCT GACGGCACTG TCGCAAACTA TCACGGCTAC CACATCGTCT 3001 TTGCATTAGC CGGAGATCCT AAAAATGCGG ATGACACATC GATTTACATG TTCTATCAAA 3061 AAGTCGGCGA AACTTCTATT GACAGCTGGA AAAACGCTGG CCGCGTCTTT AAAGACAGCG 3121 ACAAATTCGA TGCAAATGAT TCTATCCTAA AAGACCAAAC ACAAGAATGG TCAGGTTCAG 3181 CCACATTTAC ATCTGACGGA AAAATCCGTT TATTCTACAC TGATTTCTCC GGTAAACATT 3241 ACGGCAAACA AACACTGACA ACTGCACAAG TTAACGTATC AGCATCAGAC AGCTCTTTGA 3301 ACATCAACGG TGTAGAGGAT TATAAATCAA TCTTTGACGG TGACGGAAAA ACGTATCAAA 3361 ATGTACAGCA GTTCATCGAT GAAGGCAACT ACAGCTCAGG CGACAACCAT ACGCTGAGAG 3421 ATCCTCACTA CGTAGAAGAT AAAGGCCACA AATACTTAGT ATTTGAAGCA AACACTGGAA 3481 CTGAAGATGG CTACCAAGGC GAAGAATCTT TATTTAACAA AGCATACTAT GGCAAAAGCA 3541 CATCATTCTT CCGTCAAGAA AGTCAAAAAC TTCTGCAAAG CGATAAAAAA CGCACGGCTG 3601 AGTTAGCAAA CGGCGCTCTC GGTATGATTG AGCTAAACGA TGATTACACA CTGAAAAAAG 3661 TGATGAAACC GCTGATTGCA TCTAACACAG TAACAGATGA AATTGAACGC GCGAACGTCT 3721 TTAAAATGAA CGGCAAATGG TACCTGTTCA CTGACTCCCG CGGATCAAAA ATGACGATTG 3781 ACGGCATTAC GTCTAACGAT ATTTACATGC TTGGTTATGT TTCTAATTCT TTAACTGGCC 3841 CATACAAGCC GCTGAACAAA ACTGGCCTTG TGTTAAAAAT GGATCTTGAT CCTAACGATG 3901 TAACCTTTAC TTACTCACAC TTCGCTGTAC CTCAAGCGAA AGGAAACAAT GTCGTGATTA 3961 CAAGCTATAT GACAAACAGA GGATTCTACG CAGACAAACA ATCAACGTTT GCGCCAAGCT 4021 TCCTGCTGAA CATCAAAGGC AAGAAAACAT CTGTTGTCAA AGACAGCATC CTTGAACAAG 4081 GACAATTAAC AGTTAACAAA TAAAAACGCA AAAGAAAATG CCGATNNCCG GTTTATTGAC 4141 TACCGGAAGC AGTGTGACCG TGTGCTTCTC AAATGCCTCA GGCTGTCTAT GTGTGACTGT 4201 TGAGCTGTAA CAAGTTGTCT CAGGTGTTCA ATTTCATGTT CTAGTTGCTT TGTTTTACTG 4261 GTTTCACCTG TTCTATTAGG TGTTACATGC TGTTCATCTG TTACATTGTC GATCTGTTCA 4321 TGGTGAACAG CTTTAAATGC ACCAAAAACT CGTAAAAGCT CTGATGTATC TATCTTTTTT 4381 ACACCGTTTT CATCTGTGCA TATGGACAGT TTTCCCTTTG ATATCTAACG GTGAACAGTT 4441 GTTCTACTTT TGTTTGTTAG TCTTGATGCT TCACTGATAG ATACAAGAGC CATAAGAACC 4501 TCAGATCCTT CCGTATTTAG CCAGTATGTT CTCTAGTGTG GTTCGTTGTT TTTGCGTGAG 4561 CCATGAGAAC GAACCATTGA GATCATGCTT ACTTTGCATG TCACTCAAAA ATTTTGCCTC 4621 AAAACTGGTG AGCTGAATTT TTGCAGTTAA AGCATCGTGT AGTGTTTTTC TTAGTCCGTT 4681 ACGTAGGTAG GAATCTGATG TAATGGTTGT TGGTATTTTG TCACCATTCA TTTTTATCTG 4741 GTTGTTCTCA AGTTCGGTTA CGAGATCCAT TTGTCTATCT AGTTCAACTT GGAAAATCAA 4801 CGTATCAGTC GGGCGGCCTC GCTTATCAAC CACCAATTTC ATATTGCTGT AAGTGTTTAA 4861 ATCTTTACTT ATTGGTTTCA AAACCCATTG GTTAAGCCTT TTAAACTCAT GGTAGTTATT 4921 TTCAAGCATT AACATGAACT TAAATTCATC AAGGCTAATC TCTATATTTG CCTTGTGAGT 4981 TTTCTTTTGT GTTAGTTCTT TTAATAACCA CTCATAAATC CTCATAGAGT ATTTGTTTTC 5041 AAAAGACTTA ACATGTTCCA GATTATATTT TATGAATTTT TTTAACTGGA AAAGATAAGG 5101 CAATATCTCT TCACTAAAAA CTAATTCTAA TTTTTCGCTT GAGAACTTGG CATAGTTTGT 5161 CCACTGGAAA ATCTCAAAGC CTTTAACCAA AGGATTCCTG ATTTCCACAG TTCTCGTCAT 5221 CAGCTCTCTG GTTGCTTTAG CTAATACACC ATAAGCATTT TCCCTACTGA TGTTCATCAT 5281 CTGAGCGTAT TGGTTATAAG TGAACGATAC CGTCCGTTCT TTCCTTGTAG GGTTTTCAAT 5341 CGTGGGGTTG AGTAGTGCCA CACAGCATAA AATTAGCTTG GTTTCATGCT CCGTTAAGTC 5401 ATAGCGACTA ATCGCTAGTT CATTTGCTTT GAAAACAACT AATTCAGACA TACATCTCAA 5461 TTGGTCTAGG TGATTTTAAT CACTATACCA ATTGAGATGG GCTAGTCAAT GATAATTACT 5521 AGTCCTTTTC CTTTGAGTTG TGGGTATCTG TAAATTCTGC TAGACCTTTG CTGGAAAACT 5581 TGTAAATTCT GCTAGACCCT CTGTAAATTC CGCTAGACCT TTGTGTGTTT TTTTTGTTTA 5641 TATTCAAGTG GTTATAATTT ATAGAATAAA GAAAGAATAA AAAAAGATAA AAAGAATAGA 5701 TCCCAGCCCT GTGTATAACT CACTACTTTA GTCAGTTCCG CAGTATTACA AAAGGATGTC 5761 GCAAACGCTG TTTGCTCCTC TACAAAACAG AC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.