pSGAb-km plasmid

pSGAb-km plasmid

PVT91073

pSGAb-km plasmid Information

Plasmid name: pSGAb-km
Alias: Plasmid𝲏
Gene lenght:
Host: E.coli
Use(s): Gene editing
Fragment type: CRISPR
Species: Acinetobacter baumannii
Prokaryotic resistance: Kan
Screening marker: SacB
Fluorescent labeling:
Promoter: J23119(SpeI)
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F
Reverse primer:
Copy number: High
Induction:

pSGAb-km plasmid Description

pSGAb-km is a plasmid suitable for gene editing in Escherichia coli.

pSGAb-km plasmid Sequence

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataggat tttaacattt tgcgttgttc caaaagttat caacagccta gaacgtcata 241 ggaagcgatt acagacactt tatagctatc agcatgggaa cataagggca ggatgaaata 301 tgggtcttaa aacgcaaatg gtgaggtttt agaggtattt tttgaagatg attaaggcgg 361 tttgttttta aaatttttgg cggctctcag gctgcttaca ttttaaccag ttcagtgaaa 421 agttcttttt cagcaaattt ctgtttagca ccatagctaa aacttgcgtg gaacatatta 481 agaattgacc gaaatgacac aatctcaatt atattttttt tgaaaagttt tctttatcaa 541 atattttaaa tcattgattt atatataagt atgcattcat tttaataatt aatctttatt 601 taacaatgat ttatctatat tcaattgttt aattattctt actaatatta tctctatatc 661 aatatttttt atttaaaaac atatgtttag tagtgctttt gattaaagta ccagagggag 721 ggagcagagc tgaatgggaa atactcaccc tagagcgatt cttaaaaatc accctaaagt 781 attcccattc gatgtaccgt cggtcggtcg ctttcgcatc agggatgaca tcactgtatc 841 aagctgccac tgttatgatt acgattgata gcaccgcctg aacacgctca taaccgccaa 901 ttaaatgact actcattgcg tccgctactc tgttcagttc ccctagtaat agcgtttttc 961 cgatgtgtgc tagcgtcact gtacctcatc acccacacat ggacagattg agttacagac 1021 attggctaaa tttttggggt ctatgcttga caaagcgagt taaaacctta gaatttaaga 1081 caggtacatt aagcccctgt ggtgaaatca ttagcggtcg tcaaacctta atgctttcga 1141 ttgccgatat gtcagtatcg aagatctgta ccccataaat tacggtaaag ccccaagcaa 1201 ttgcaagggg cttttatctt ttttaacaaa aaaaatttat aaatcaggat tttataacac 1261 taaataatcc aaagtacatg agtaagtcat gaccactcct gcgatgtgtg tagcactctg 1321 agtatccgta ttatatcagt gatgtcatat acaaccacat aatgcggatg tatcactaac 1381 tccctagtat ctttctgtct gcctgtgcgc cccatcagtc gattatcaac aagtaaatct 1441 gtcttttctt caattaaatc atcaatttca atagctgcta tagggttgcg ttcttcaaga 1501 taatcataga tatttctacg atcttggcta tgcggtgtga aataccgcac agatgcgtaa 1561 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 1621 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 1681 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 1741 aattgacgcg tattgggatg gtaccgggcc ccccctcgag gtcgacggta tcgatacggc 1801 atcagagcag attgtactga gagtgcacca taatcggcat tttcttttgc gtttttattt 1861 gttaactgtt aattgtcctt gttcaaggat gctgtctttg acaacagatg ttttcttgcc 1921 tttgatgttc agcaggaagc taggcgcaaa cgttgattgt ttgtctgcgt agaatcctct 1981 gtttgtcata tagcttgtaa tcacgacatt gtttcctttc gcttgaggta cagcgaagtg 2041 tgagtaagta aaggttacat cgttaggatc aagatccatt tttaacacaa ggccagtttt 2101 gttcagcggc ttgtatgggc cagttaaaga attagaaaca taaccaagca tgtaaatatc 2161 gttagacgta atgccgtcaa tcgtcatttt tgatccgcgg gagtcagtga acagatacca 2221 tttgccgttc attttaaaga cgttcgcgcg ttcaatttca tctgttactg tgttagatgc 2281 aatcagcggt ttcatcactt ttttcagtgt gtaatcatcg tttagctcaa tcataccgag 2341 agcgccgttt gctaactcag ccgtgcgttt tttatcgctt tgcagaagtt tttgactttc 2401 ttgacggaag aatgatgtgc ttttgccata gtatgctttg ttaaataaag attcttcgcc 2461 ttggtagcca tcttcagttc cagtgtttgc ttcaaatact aagtatttgt ggcctttatc 2521 ttctacgtag tgaggatctc tcagcgtatg gttgtcgcct gagctgtagt tgccttcatc 2581 gatgaactgc tgtacatttt gatacgtttt tccgtcaccg tcaaagattg atttataatc 2641 ctctacaccg ttgatgttca aagagctgtc tgatgctgat acgttaactt gtgcagttgt 2701 cagtgtttgt ttgccgtaat gtttaccgga gaaatcagtg tagaataaac ggatttttcc 2761 gtcagatgta aatgtggctg aacctgacca ttcttgtgtt tggtctttta ggatagaatc 2821 atttgcatcg aatttgtcgc tgtctttaaa gacgcggcca gcgtttttcc agctgtcaat 2881 agaagtttcg ccgacttttt gatagaacat gtaaatcgat gtgtcatccg catttttagg 2941 atctccggct aatgcaaaga cgatgtggta gccgtgatag tttgcgacag tgccgtcagc 3001 gttttgtaat ggccagctgt cccaaacgtc caggcctttt gcagaagaga tatttttaat 3061 tgtggacgaa tcgaactcag gaacttgata tttttcattt ttttgctgtt cagggatttg 3121 cagcatatca tggcgtgtaa tatgggaaat gccgtatgtt tccttatatg gcttttggtt 3181 cgtttctttc gcaaacgctt gagttgcgcc tcctgccagc agtgcggtag taaaggttaa 3241 tactgttgct tgttttgcaa actttttgat gttcatcgtt catgtctcct tttttatgta 3301 ctgtgttagc ggtctgcttc ttccagccct cctgtttgaa gatggcaagt tagttacgca 3361 caataaaaaa agacctaaaa tatgtaaggg gtgacgccaa agtatacact ttgcccttta 3421 cacattttag gtcttgcctg ctttatcagt aacaaacccg cgcgatttac ttttcgacct 3481 cattctatta gactctcgtt tggattgcaa ctggtctatt ttcctctttt gtttgataga 3541 aaatcataaa aggatttgca gactacgggc ctaaagaact aaaaaatcta tctgtttctt 3601 ttcattctct gtatttttta tagtttctgt tgcatgggca taaagttgca agcttgatat 3661 cttgacagct agctcagtcc taggtataat actagtcgag accattggtc tcagttttag 3721 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg 3781 agtcggtgct ttttttgata tcgaattcct gcagcccggg ggatccacta gttctagagc 3841 ggccgccacc gcggtggagc tcatcccaat ggcgcgccga gcttggctcg agcatggtca 3901 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 3961 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 4021 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 4081 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 4141 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 4201 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 4261 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 4321 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 4381 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 4441 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 4501 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 4561 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 4621 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 4681 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 4741 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 4801 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 4861 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 4921 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 4981 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 5041 taaacttggt ctgacagtta gaaaaactca tcgagcatca aatgaaactg caatttattc 5101 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac 5161 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt 5221 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa 5281 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag 5341 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg 5401 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa 5461 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt 5521 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccagg gatcgcagtg 5581 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata 5641 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct 5701 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc 5761 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg 5821 ttggaattta atcgcggcct agagcaagac gtttcccgtt gaatatggct catactcttc 5881 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 5941 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 6001 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 6061 aggccctttc gtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.