pLV3-CMV-HP1BP3-circRNA-CopGFP

pLV3-CMV-HP1BP3-circRNA-CopGFP-Puro plasmid

PVT96484

pLV3-CMV-HP1BP3-circRNA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-HP1BP3(human)-circRNA-CopGFP-Puro
Alias: -
Gene lenght: 160bp
Host: Mammalian cells,lentivirus
Use(s): Transposition regulation
Fragment type: circRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HP1BP3-circRNA-CopGFP-Puro plasmid Description

pLV3-CMV-HP1BP3-circRNA-CopGFP-Puro A plasmid suitable for transcriptional regulation in mammalian cells, also applicable for lentivirus packaging.

pLV3-CMV-HP1BP3-circRNA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagatTA 2281 ATACTTTCAG aattcGCCAC CCTGAAGAAA TCAGGGGAGA AACCCCTGCT TGGTGGAAGC 2341 CTGATGGAAT ATGCAATCTT GTCTGCCATT GCTGCCATGA ATGAGCCGAA GACCTGCTCT 2401 ACCACTGCTC TGAAGAAGTA TGTCCTAGAG AATCACCCAG GAACCAATTC TAACTATCAA 2461 ACTCGAGTAA GAACAACTGa aggatctgcg atcgctccgg tgcccgtcag tgggcagagc 2521 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct 2581 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 2641 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 2701 acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg 2761 cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg 2821 cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac 2881 cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt 2941 gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca 3001 gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac gagagcggcc 3061 tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg gagttcgagc 3121 tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag atgaagagca 3181 ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc tacggcttct 3241 accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc atcaacaacg 3301 gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg cacgtgagct 3361 tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg ggcaccggct 3421 tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc accgtggagc 3481 acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc 3541 gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag agcgccatcc 3601 accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc 3661 acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc cccatcgcct 3721 tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct 3781 ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc 3841 ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca 3901 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg 3961 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg 4021 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca 4081 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt 4141 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc 4201 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg 4261 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg 4321 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg 4381 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg 4441 gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 4501 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 4561 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 4621 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 4681 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 4741 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 4801 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat 4861 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 4921 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 4981 cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgcctgg 5041 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 5101 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta 5161 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 5221 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 5281 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 5341 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 5401 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 5461 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 5521 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc 5581 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 5641 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg 5701 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 5761 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 5821 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 5881 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 5941 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6001 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 6061 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 6121 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 6181 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 6241 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6301 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 6361 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 6421 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 6481 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 6541 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 6601 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 6661 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 6721 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 6781 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 6841 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 6901 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 6961 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 7021 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 7081 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 7141 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 7201 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 7261 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 7321 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 7381 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 7441 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 7501 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 7561 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 7621 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 7681 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 7741 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 7801 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 7861 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 7921 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 7981 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 8041 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 8101 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 8161 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 8221 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 8281 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 8341 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.