pAAV-hSyn-Serinc1-EGFP plasmid

pAAV-hSyn-Serinc1-EGFP plasmid

PVT90316

pAAV-hSyn-Serinc1-EGFP plasmid Information

Plasmid name: pAAV-hSyn-Serinc1(mouse)-EGFP
Alias: NM_019760.4;1500011D18Rik; AIGP-2; mKIAA1253; Tde1l;Tde2; TMS-2; Tms2
Gene lenght: 1359bp
Host: Mammalian cells,Adeno associated virus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: hSyn
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: pEGFP-N-3'
Copy number: High
Induction:

pAAV-hSyn-Serinc1-EGFP plasmid Description

pAAV-hSyn-Serinc1-EGFP This is a plasmid suitable for protein expression in mammalian cells and can also be used for adeno-associated virus packaging.

pAAV-hSyn-Serinc1-EGFP plasmid Sequence

1 CATGTCCTGC AGGCAGCTGC GCGCTCGCTC GCTCACTGAG GCCGCCCGGG CGTCGGGCGA 61 CCTTTGGTCG CCCGGCCTCA GTGAGCGAGC GAGCGCGCAG AGAGGGAGTG GCCAACTCCA 121 TCACTAGGGG TTCCTGCGGC CGCACGCGTG TGTCTAGACT GCAGAGGGCC CTGCGTATGA 181 GTGCAAGTGG GTTTTAGGAC CAGGATGAGG CGGGGTGGGG GTGCCTACCT GACGACCGAC 241 CCCGACCCAC TGGACAAGCA CCCAACCCCC ATTCCCCAAA TTGCGCATCC CCTATCAGAG 301 AGGGGGAGGG GAAACAGGAT GCGGCGAGGC GCGTGCGCAC TGCCAGCTTC AGCACCGCGG 361 ACAGTGCCTT CGCCCCCGCC TGGCGGCGCG CGCCACCGCC GCCTCAGCAC TGAAGGCGCG 421 CTGACGTCAC TCGCCGGTCC CCCGCAAACT CCCCTTCCCG GCCACCTTGG TCGCGTCCGC 481 GCCGCCGCCG GCCCAGCCGG ACCGCACCAC GCGAGGCGCG AGATAGGGGG GCACGGGCGC 541 GACCATCTGC GCTGCGGCGC CGGCGACTCA GCGCTGCCTC AGTCTGCGGT GGGCAGCGGA 601 GGAGTCGTGT CGTGCCTGAG AGCGCAGTCG AGAAGGTACC gccaccatgg ggagcgttct 661 ggggctgtgc tccgtggcga gctggattcc gtgtttgtgt ggcagtgccc cgtgtttgct 721 gtgccgatgc tgccccagtg ggaacaactc cactgtgact agattgatct acgcactttt 781 cttgctcgtc ggagtatgtg tagcttgtgt aatgttaata ccaggaatgg aagaacaact 841 gaataagatt cctgggttct gtgagaatga gaaaggtgtt gttccctgta atatcctggt 901 tggctacaaa gctgtgtatc gcctgtgctt tggtttggcc atgttctatc ttctcctctc 961 tttactaatg atcaaggtaa aaagcagcag tgaccctaga gcggcagtcc acaatggatt 1021 ctggttcttt aaatttgcta cagcagttgc aattatcatt ggcgcattct tcatcccaga 1081 aggcactttt acaactgtgt ggttttatgt aggcatggcg ggcgcctttt gtttcatcct 1141 tatacaacta gtcttactta ttgattttgc ccattcttgg aatgaatcgt gggttgaaaa 1201 aatggaagaa gggaactcaa gatgttggta tgcagcccta ctgtcagcta cagctctgaa 1261 ttacttgctg tctttagttg ccgtcgtgct attctttgtc tactacactc acccagccag 1321 ctgtgcagag aacaaggcgt tcatcagtgt caacatgctc ctctgcattg gcgcctcagt 1381 aatgtccatt ctgcccaaga tccaggaatc acaaccaagg tctggcttgt tacagtcgtc 1441 agtaattaca gtctacacaa tgtatttgac atggtctgct atgaccaatg aaccagaaac 1501 aaattgcaat ccaagtctac tgagcatcat tggcttcaat accacaaggc ctattccaaa 1561 ggacgggcag tctgttcagt ggtggcatcc tcaaggaatt ataggacttg ttcttttcct 1621 gctgtgtgtg ttttattcga gcatccgtac ctccaacaac agtcaggtta ataagctgac 1681 tctaacaagt gatgagtcaa cattgataga ggatgggaat ggcagaagtg acggatcact 1741 agatgatggt gatggcatcc accgagctgt agataacgag agggatggag tcacgtacag 1801 ttactccttc tttcacttca tgctcttctt ggcttcactt tacatcatga tgaccctcac 1861 caattggtac aggtatgagc cttctcgtga gatgaaaagt cagtggacag cagtctgggt 1921 gaaaatctct tccagttgga ttggccttgt gctgtacgtg tggacactag tggcaccact 1981 tgttcttaca aatcgggatt ttgacGCCAC TAACTTCTCC CTGTTGAAAC AAGCAGGGGA 2041 TGTCGAAGAG AATCCCGGGC CAGGATCCGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT 2101 CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG 2161 CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG 2221 CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT 2281 GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT 2341 GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC 2401 CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT 2461 CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA 2521 CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG 2581 CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT 2641 CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG 2701 CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG 2761 GATCACTCTC GGCATGGACG AGCTGTACAA GTAGGAATTC GATATCAAGC TTATCGATAA 2821 TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC 2881 TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT 2941 GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG 3001 GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG 3061 TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT 3121 TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT 3181 GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC 3241 CTATGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA 3301 TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG 3361 CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCATC GATACCGAGC 3421 GCTGCTCGAG AGATCTACGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 3481 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 3541 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 3601 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 3661 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 3721 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 3781 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 3841 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 3901 CCCTTCCCTG TCCTTCTGAT TTTGTAGGTA ACCACGTGCG GACCGAGCGG CCGCAGGAAC 3961 CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG CTCGCTCACT GAGGCCGGGC 4021 GACCAAAGGT CGCCCGACGC CCGGGCTTTG CCCGGGCGGC CTCAGTGAGC GAGCGAGCGC 4081 GCAGCTGCCT GCAGGGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT 4141 CACACCGCAT ACGTCAAAGC AACCATAGTA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC 4201 GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCTTAG CGCCCGCTCC 4261 TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA 4321 TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT 4381 TGATTTGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT 4441 GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA 4501 CTCTATCTCG GGCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG TCTATTGGTT 4561 AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGTTTAC 4621 AATTTTATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG 4681 ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA 4741 CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC 4801 GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT 4861 AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT 4921 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA 4981 AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT 5041 TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA 5101 AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA 5161 CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT 5221 TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG 5281 TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA 5341 TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA 5401 CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT 5461 GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC 5521 CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA 5581 ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA 5641 GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC 5701 TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA 5761 TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA 5821 ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA 5881 CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT 5941 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT 6001 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 6061 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 6121 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 6181 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 6241 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 6301 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 6361 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 6421 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 6481 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 6541 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 6601 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 6661 CCTGGCCTTT TGCTGGCCTT TTGCTCA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.