pLV3-CMV-HNF4A-3×FLAG-CopGFP-P

pLV3-CMV-HNF4A-3×FLAG-CopGFP-Puro plasmid

PVT96849

pLV3-CMV-HNF4A-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-HNF4A(human)-3×FLAG-CopGFP-Puro
Alias: NM_000457.6;FRTS4; HNF4; HNF4a7; HNF4a8; HNF4a9;HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF-14; TCF14
Gene lenght: 1422bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HNF4A-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-HNF4A-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-HNF4A-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc gactctccaa aaccctcgtc 2341 gacatggaca tggccgacta cagtgctgca ctggacccag cctacaccac cctggaattt 2401 gagaatgtgc aggtgttgac gatgggcaat gacacgtccc catcagaagg caccaacctc 2461 aacgcgccca acagcctggg tgtcagcgcc ctgtgtgcca tctgcgggga ccgggccacg 2521 ggcaaacact acggtgcctc gagctgtgac ggctgcaagg gcttcttccg gaggagcgtg 2581 cggaagaacc acatgtactc ctgcagattt agccggcagt gcgtggtgga caaagacaag 2641 aggaaccagt gccgctactg caggctcaag aaatgcttcc gggctggcat gaagaaggaa 2701 gccgtccaga atgagcggga ccggatcagc actcgaaggt caagctatga ggacagcagc 2761 ctgccctcca tcaatgcgct cctgcaggcg gaggtcctgt cccgacagat cacctccccc 2821 gtctccggga tcaacggcga cattcgggcg aagaagattg ccagcatcgc agatgtgtgt 2881 gagtccatga aggagcagct gctggttctc gttgagtggg ccaagtacat cccagctttc 2941 tgcgagctcc ccctggacga ccaggtggcc ctgctcagag cccatgctgg cgagcacctg 3001 ctgctcggag ccaccaagag atccatggtg ttcaaggacg tgctgctcct aggcaatgac 3061 tacattgtcc ctcggcactg cccggagctg gcggagatga gccgggtgtc catacgcatc 3121 cttgacgagc tggtgctgcc cttccaggag ctgcagatcg atgacaatga gtatgcctac 3181 ctcaaagcca tcatcttctt tgacccagat gccaaggggc tgagcgatcc agggaagatc 3241 aagcggctgc gttcccaggt gcaggtgagc ttggaggact acatcaacga ccgccagtat 3301 gactcgcgtg gccgctttgg agagctgctg ctgctgctgc ccaccttgca gagcatcacc 3361 tggcagatga tcgagcagat ccagttcatc aagctcttcg gcatggccaa gattgacaac 3421 ctgttgcagg agatgctgct gggagggtcc cccagcgatg caccccatgc ccaccacccc 3481 ctgcaccctc acctgatgca ggaacatatg ggaaccaacg tcatcgttgc caacacaatg 3541 cccactcacc tcagcaacgg acagatgtgt gagtggcccc gacccagggg acaggcagcc 3601 acccctgaga ccccacagcc ctcaccgcca ggtggctcag ggtctgagcc ctataagctc 3661 ctgccgggag ccgtcgccac aatcgtcaag cccctctctg ccatccccca gccgaccatc 3721 accaagcagg aagttatcgg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 3781 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 3841 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 3901 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 3961 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 4021 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 4081 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 4141 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 4201 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 4261 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 4321 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 4381 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 4441 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 4501 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 4561 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 4621 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 4681 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 4741 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 4801 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 4861 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 4921 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 4981 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 5041 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 5101 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 5161 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 5221 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 5281 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 5341 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 5401 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 5461 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5521 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5581 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5641 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 5701 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5761 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 5821 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 5881 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 5941 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 6001 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 6061 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 6121 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 6181 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 6241 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6301 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 6361 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 6421 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 6481 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 6541 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6601 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6661 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6721 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 6781 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 6841 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 6901 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 6961 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 7021 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 7081 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 7141 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 7201 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 7261 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 7321 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 7381 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 7441 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 7501 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 7561 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 7621 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 7681 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 7741 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 7801 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 7861 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 7921 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 7981 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 8041 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 8101 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 8161 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 8221 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 8281 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 8341 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 8401 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 8461 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 8521 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 8581 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 8641 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 8701 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 8761 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 8821 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 8881 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 8941 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 9001 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 9061 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 9121 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 9181 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 9241 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 9301 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 9361 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 9421 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 9481 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 9541 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 9601 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 9661 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.