pCMV-EGFP-Mms19-3×FLAG-Neo pla

pCMV-EGFP-Mms19-3×FLAG-Neo plasmid

PVT94844

pCMV-EGFP-Mms19-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-EGFP-Mms19(mouse)-3×FLAG-Neo
Alias: NM_028152.3;2410001K24Rik; 2610042O15Rik; Mms19l
Gene lenght: 3093bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-Mms19-3×FLAG-Neo plasmid Description

pCMV-EGFP-Mms19-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-Mms19-3×FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTgcca ccatggtgag caagggcgag gagctgttca ccggggtggt 961 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 1021 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 1081 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 1141 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 1201 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 1261 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 1321 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 1381 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 1441 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 1501 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 1561 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 1621 catggacgag ctgtacaagg gcggtggagg cagtggtgga ggcggtagcG AATTCatggc 1681 tgccgccacc ggtctggagg aggcagtggc gcctatgggc gccctgtgtg gcctcgtgca 1741 agacttcgtc atgggtcaac aggaggggcc cgctgaccag gtggctgcag atgtgaagtc 1801 cggtggctat acagtgttac aagtagtaga agcccttggg tcctctctgg aaaatgctga 1861 accccgaact cgggctcgag gagcccagct tctgtcacag gtgctgctcc agtgtcactc 1921 cttgctctcg gagaaggaag tggtccacct gatcctgttc tatgagaacc ggctgaagga 1981 ccaccatctt gtggtcccat ctgtcttaca gggcctgagg gcactgagca tgtctgtggc 2041 cctgcctccg ggtctggctg tctctgtgct taaagccatc ttccaggagg tacatgtaca 2101 gtccctgcta caggtggacc gccatactgt cttcagcatc atcactaact tcatgcgatc 2161 acgagaagaa gagctgaagg gtctgggagc tgacttcaca tttggcttca tccaggtgat 2221 ggatggggag aaggatcccc gtaatctcct gctggctttc cgcatcgtcc atgacctcat 2281 ctccaaggac tacagtctgg gacctttcgt ggaagagtta tttgaagtga catcttgtta 2341 tttccctatt gattttaccc ctccgcctaa cgatccttat ggcatccaga gagaggatct 2401 catcctgagt ctccgtgctg tgctggcgtc tacaccgcgc tttgccgagt tcttactgcc 2461 tctgctaatt gagaaagtgg attcggagat tttaagtgcc aagctggatt ctctgcaaac 2521 tctgaatgct tgctgtgctg tgtatggaca gaaggagctg aaggacttcc tgcccagcct 2581 ttgggcttcc atccgtagag aggttttcca gactgctagt gagcgagtag aggcagaggg 2641 cctggcagct ctccactccc tgactgcgtg tctgtcctgc tctgtgctga gagctgatgc 2701 tgaggacctc cttggttcct tcctcagcaa catccttcaa gactgcaggc accatctatg 2761 tgaaccagac atgaaacttg tatggcctag cgctaagctg ttgcaggcag ctgcaggtgc 2821 gtctgcccgg gcctgtgagc acctcaccag caacgtgctt cccttgctgc tggaacagtt 2881 ccacaagcac agccagagca accagcgtcg gacaatcctt gaaatgatcc tgggattcct 2941 aaagctacag cagaaatgga gctatgaaga cagagatgaa aggcctctga gtagcttcaa 3001 agaccagcta tgctcattgg tgttcatggc tctaacagac cccagcaccc agcttcagct 3061 tgttggcatc cggacgctca cagtcttggg tgcccagcca ggtctcctgt ctgctgagga 3121 cttggagctg gcagtaggtc acctgtaccg actgaccttc ctggaggaag attcccagag 3181 ttgtagggtg gccgcactgg aggcatcagg aaccctggcc actctgtatc ctggagcctt 3241 cagcagacac ttgctcccca agcttgctga ggagctgcat aaaggggagt cagatgtggc 3301 tagagcagat ggtcccacca aatgttcccg gcatttccgc tgtctgcaag ctttgtcagc 3361 tgtttcaaca catcccagca ttgtcaagga gacactgcct ctgctactgc agcatctctg 3421 tcaagcaaac aaagggaata tggttacaga gtccagtgaa gttgttgctg tctgtcagag 3481 tctccagcag gtggcagaaa aatgccagca ggaccctgag agctactggt atttccacaa 3541 gacagctgta ccctgcctgt ttgcattggc tgtgcaggct tcaatgccag agaaggaatc 3601 ctcagttctg agaaaagtac tgttggagga tgaggtcttg gctgcgttgg catctgtcat 3661 tggcactgcc actacccatc tgagccctga actagctgcc cagagcgtca catgcatcgt 3721 gcccctcttc ttagatggca acacttcctt tctacctgaa aacagcttcc ctgaccagtt 3781 ccagccgttc caggatggct cctctgggca gaggcggctg gttgcactac ttacggcttt 3841 tgtctgctcc ctgccacgaa atgtggaaat ccctcagctg aaccgactca tgcgggagct 3901 tttgaagcag agctgtggcc acagctgtcc tttctcctcc actgccgcca ctaagtgctt 3961 tgcagggctc ctcaacaagc agcctccagg gcagcagttg gaggagttcc tccagctcgc 4021 tgtgggcaca gtggaggcgg gcctggcctc tgaatcctcc cgtgatcagg ctttcacact 4081 gctgctctgg gtaaccaagg ctctagtcct cagataccat cctctcagtg cctgcctgac 4141 cacccggctc atgggcctcc tcagtgatcc agaacttggc tgtgcagcag ctgatggctt 4201 ctctctgctc atgtctgact gcactgatgt gttgactcgt gccggccatg ctgatgttcg 4261 gatcatgttc cgccagcggt tcttcacaga caatgtgcct gctttggtcc aaggtttcca 4321 tgccgctccc caagatgtga agccaaacta tctgaagggt ctgtctcacg tactcaacag 4381 gctgcccaag cctgtgcttt taccagagct gcccacactt ctttccttgc tgctggaagc 4441 attatcctgc cctgactctg tggtccagct ttccactctg agctgtctcc aacccctact 4501 actggaagca cctcaaatca tgagtcttca tgttgacact ctggttacaa aattcctgaa 4561 cctcagctcc agttactcaa tggctgtccg gattgctgct ctgcagtgta tgcacgctct 4621 cactcgcctc cccacttctg tgctactgcc atacaaatct caggtcatcc gagccttagc 4681 caagcctctg gacgacaaga agagacttgt gcgaaaggaa gcagtgtcag cgaggggaga 4741 atggttccta cttgggagcc cggggagcCT CGAGGACTAC AAAGACCATG ACGGTGATTA 4801 TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG TAGTGAGGGC CCGTTTAAAC 4861 CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC 4921 CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA 4981 AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA 5041 CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT 5101 GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG 5161 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG 5221 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT 5281 TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA 5341 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA 5401 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA 5461 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC 5521 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT 5581 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 5641 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 5701 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 5761 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 5821 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG 5881 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 5941 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 6001 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 6061 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 6121 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 6181 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 6241 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 6301 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 6361 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 6421 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 6481 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 6541 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 6601 TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC 6661 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 6721 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 6781 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 6841 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 6901 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 6961 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 7021 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 7081 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 7141 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 7201 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 7261 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 7321 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 7381 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 7441 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 7501 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 7561 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 7621 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 7681 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 7741 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 7801 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 7861 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 7921 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 7981 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 8041 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 8101 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 8161 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 8221 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 8281 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 8341 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 8401 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 8461 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 8521 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 8581 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 8641 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 8701 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 8761 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 8821 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 8881 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 8941 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 9001 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 9061 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 9121 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 9181 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 9241 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.