pLV3-CMV-IRF8-2-3×FLAG-CopGFP-

pLV3-CMV-IRF8-2-3×FLAG-CopGFP-Puro plasmid

PVT96822

pLV3-CMV-IRF8-2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-IRF8(human)-2-3×FLAG-CopGFP-Puro
Alias: NM_001363907.1;H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B;IRF-8
Gene lenght: 1308bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-IRF8-2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-IRF8-2-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-IRF8-2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg caggtgtccc ggagtccctg 2341 aatctgatgt gtgaccggaa tggtggtcgg cggcttcgac agtggctgat cgagcagatt 2401 gacagtagca tgtatccagg actgatttgg gagaatgagg agaagagcat gttccggatc 2461 ccttggaaac acgctggcaa gcaagattat aatcaggaag tggatgcctc catttttaag 2521 gcctgggcag tttttaaagg gaagtttaaa gaaggggaca aagctgaacc agccacttgg 2581 aagacgaggt tacgctgtgc tttgaataag agcccagatt ttgaggaagt gacggaccgg 2641 tcccaactgg acatttccga gccatacaaa gtttaccgaa ttgttcctga ggaagagcaa 2701 aaatgcaaac taggcgtggc aactgctggc tgcgtgaatg aagttacaga gatggagtgc 2761 ggtcgctctg aaatcgacga gctgatcaag gagccttctg tggacgatta catggggatg 2821 atcaaaagga gcccttcccc gccggaggcc tgtcggagtc agctccttcc agactggtgg 2881 gcgcagcagc ccagcacagg cgtgccgctg gtgacggggt acaccaccta cgacgcgcac 2941 cattcagcat tctcccagat ggtgatcagc ttctactatg ggggcaagct ggtgggccag 3001 gccaccacca cctgccccga gggctgccgc ctgtccctga gccagcctgg gctgcccggc 3061 accaagctgt atgggcccga gggcctggag ctggtgcgct tcccgccggc cgacgccatc 3121 cccagcgagc gacagaggca ggtgacgcgg aagctgttcg ggcacctgga gcgcggggtg 3181 ctgctgcaca gcagccggca gggcgtgttc gtcaagcggc tgtgccaggg ccgcgtgttc 3241 tgcagcggca acgccgtggt gtgcaaaggc aggcccaaca agctggagcg tgatgaggtg 3301 gtccaggtct tcgacaccag ccagttcttc cgagagctgc agcagttcta taacagccag 3361 ggccggcttc ctgacggcag ggtggtgctg tgctttgggg aagagtttcc ggatatggcc 3421 cccttgcgct ccaaactcat tctcgtgcag attgagcagc tgtatgtccg gcaactggca 3481 gaagaggctg ggaagagctg tggagccggc tctgtgatgc aggcccccga ggagccgccg 3541 ccagaccagg tcttccggat gtttccagat atttgtgcct cacaccagag atcatttttc 3601 agagaaaacc aacagatcac cgtcggatcc GACTACAAAG ACCATGACGG TGATTATAAA 3661 GATCATGACA TCGACTACAA GGATGACGAT GACAAGtagc ggccgcGaag gatctgcgat 3721 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 3781 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 3841 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 3901 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 3961 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 4021 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 4081 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 4141 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 4201 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac 4261 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc 4321 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag 4381 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg 4441 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag 4501 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac 4561 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc 4621 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag 4681 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg 4741 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac 4801 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg 4861 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac 4921 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct 4981 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt 5041 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg 5101 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 5161 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 5221 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 5281 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 5341 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 5401 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 5461 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 5521 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 5581 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 5641 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt 5701 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 5761 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 5821 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 5881 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 5941 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 6001 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 6061 ttgtcgggga aATCATCGTC CTTTCCTtgg ctgctcgcct gtgttgccac ctggattctg 6121 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 6181 ggcctgctgc cggctctgcg gcctcttccg cgtctTcgcc ttcgccctca gacgagtcgg 6241 atctcccttt ggGccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 6301 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 6361 acgaaGataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 6421 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 6481 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 6541 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 6601 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 6661 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 6721 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 6781 gctaTCCCGC CCCTAACTCC GCCCAtcccg cccctaactc cgcccagttc cgcccattct 6841 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 6901 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa 6961 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 7021 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 7081 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 7141 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 7201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 7261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 7561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 7621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 7681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 7741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 7801 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 7861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 7921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 7981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 8041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 8101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 8161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 8221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 8281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 8341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 8401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 8461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 8521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 8581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 8641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 8701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 8761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 8821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 8881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 8941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 9001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 9061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 9121 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 9181 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 9241 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 9301 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 9361 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 9421 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 9481 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 9541 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.