pCMV-EGFP-Insr-Neo plasmid

pCMV-EGFP-Insr-Neo plasmid

PVT95390

pCMV-EGFP-Insr-Neo plasmid Information

Plasmid name: pCMV-EGFP-Insr(mouse)-Neo
Alias: NM_010568.3;4932439J01Rik; CD220; D630014A15Rik; IR;IR-A; IR-B
Gene lenght: 4119bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-Insr-Neo plasmid Description

pCMV-EGFP-Insr-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-Insr-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCgcc accatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag gGaGGCGGAG GTAGTGGAGG AGGGGGAAGC 1621 ggaggcggag ggagtGAATT Catgggcttc gggagaggat gtgagacgac ggctgtgcca 1681 ttgctggtgg ccgtggccgc gttgctggtg ggcacagccg gccacctgta ccctggagag 1741 gtgtgccctg gtatggacat ccggaacaac ctgaccaggc tacatgagct ggagaactgc 1801 tcagtcattg agggccatct gcagatcctc ctgatgttca agaccagacc cgaagatttc 1861 cgagacctca gtttccccaa actcatcatg atcacagatt acctgcttct cttccgtgtc 1921 tatggtctgg aaagtctgaa agacctcttc ccaaatctca cagtcatccg aggctcccgt 1981 ctcttcttca actatgccct ggttatcttc gagatggtcc acctgaagga gctggggctt 2041 tataacctca tgaacatcac ccggggctct gtccgcatcg agaagaataa tgagctctgc 2101 tacctggcca ctatcgactg gtcccgtatc ctggattctg tggaggacaa ctacattgta 2161 ctgaacaaag atgacaacga ggaatgtggg gatgtctgtc caggcaccgc caagggcaag 2221 accaactgtc ctgccactgt catcaatggg cagtttgtgg aacggtgctg gacacacagt 2281 cattgtcaga aagtttgccc aaccatctgt aagtcacatg gctgcacagc tgaaggcctg 2341 tgctgccaca aagagtgcct gggcaactgt tcggaacctg atgaccccac caagtgtgtg 2401 gcctgtcgca acttctatct ggatggtcag tgtgtggaga cctgcccgcc accctactat 2461 cacttccagg actggcgctg tgtgaacttc agcttctgcc aagaccttca cttcaaatgc 2521 aggaactctc ggaagcctgg ctgccaccaa tacgtcattc acaacaataa gtgcatcccc 2581 gagtgcccgt ctggctatac catgaattcc agcaacttga tgtgcacccc atgtctggga 2641 ccctgcccta aggtctgcca aatcctcgaa ggtgagaaga ccattgattc tgtgacatct 2701 gcccaggagc tccgaggctg cactgtgatc aacggtagcc tgatcatcaa catccgaggg 2761 ggcaacaacc tggcagctga gctggaggct aaccttggcc tcattgaaga aatttcggga 2821 tttctaaaga tccgccgctc ctatgctctg gtatcacttt ctttcttcag gaagctacat 2881 ctgattcgag gagagacctt ggaaattggg aactattctt tttatgcctt ggacaaccag 2941 aacctgaggc aactctggga ctggagcaaa cacaacctca ccatcactca gggcaagctc 3001 ttcttccatt acaacccgaa actctgcttg tctgaaattc acaagatgga agaagtctcc 3061 ggaactaagg gccgtcagga gaggaacgac attgccctga agaccaatgg ggaccaggca 3121 tcgtgtgaaa atgaattgct taaattttct ttcattcgga catcttttga caagatcctg 3181 ttgaggtggg aaccctactg gccccccgac ttccgagatc tcctgggatt catgttgttc 3241 tacaaagagg ccccttatca gaatgtgaca gagtttgatg ggcaggatgc ttgtggctcc 3301 aacagctgga ctgtggtgga tattgacccg ccccagaggt ccaacgaccc caagtctcag 3361 accccaagcc accctgggtg gctgatgcgg ggcctcaaac cctggaccca atacgccatc 3421 tttgtgaaga ccttggttac cttctctgat gaacggcgga cctatggagc caaaagtgat 3481 atcatctatg tgcaaacaga tgccactaat ccttctgtcc ccctggatcc catatcagtt 3541 tctaattcct catctcagat tatcttaaag tggaagcccc cctctgaccc caatggcaac 3601 atcacacact acctggtgta ctgggagagg caagcagagg acagcgagct gtttgagctg 3661 gattattgtc tcaaagggct gaagctccct tcacggacct ggtccccacc ctttgagtct 3721 gatgattctc agaagcacaa tcagagtgag tatgacgact cggccagtga gtgctgctca 3781 tgccctaaga ctgactctca gatcctgaag gagctggagg agtcttcatt caggaagacc 3841 ttcgaggatt acctgcacaa cgtggttttt gtccccaggc catcccgaaa gcgaagatcc 3901 cttgaagagg tggggaatgt gacagccacc acactcacac ttccagattt ccccaacgtc 3961 tcctctacca ttgtgcccac aagtcaggag gagcacaggc catttgagaa agtggtgaac 4021 aaggagtcac ttgtcatctc tggcctgaga cacttcactg ggtaccgcat tgagctgcag 4081 gcatgcaatc aagattcccc agatgagagg tgcagtgtgg ctgcctacgt cagtgcccgg 4141 accatgcctg aagctaaggc agatgacatc gttggccctg tgactcatga aatctttgag 4201 aacaatgttg tacacttaat gtggcaagag ccaaaggaac ctaatggtct gattgtgcta 4261 tatgaagtga gctatcgccg atatggtgat gaggagctgc acctctgtgt ctcccggaag 4321 cattttgccc tggagcgggg ctgcaggctg cgagggctct ccccaggaaa ctacagtgtt 4381 cgagtccggg ctacctctct ggcaggaaat ggctcctgga cagaacccac ctatttttat 4441 gtgactgatt atttagatgt cccatcaaat attgccaaaa ttatcattgg acccctcatc 4501 tttgtcttcc tcttcagtgt tgtgattgga agtatttatc tatttctgag aaagaggcag 4561 ccggatgggc caatgggacc actgtatgca tcttcaaacc ctgagtacct cagtgccagt 4621 gatgtgtttc catcttctgt gtacgtgccg gacgagtggg aggtgcctcg agagaagatc 4681 acccttcttc gagagctggg gcagggatcc tttggtatgg tgtatgaagg caatgccaag 4741 gatatcatca agggtgaggc agagacccgt gttgcggtta agactgtcaa tgagtcagcc 4801 agtcttcgag aacggatcga gttcctcaat gaggcatcag tcatgaaggg attcacctgc 4861 catcatgtgg tccgccttct tggggtggta tccaaaggac agccaacgct ggtagtgatg 4921 gaattgatgg ctcatggaga cctgaaaagt cacctccgtt ctctgaggcc agatgctgag 4981 aataacccag gccgccctcc ccctaccttg caagaaatga ttcagatgac agcagaaatt 5041 gctgatggca tggcatactt gaacgccaag aagtttgtgc accgggacct ggcagctcga 5101 aactgcatgg ttgcccatga ttttactgtc aaaattggag actttggaat gacaagggac 5161 atctacgaga cagattacta tcggaaaggg ggcaagggac tgcttcctgt gaggtggatg 5221 tcacctgagt ccctgaagga tggagtcttt actgcttctt ctgatatgtg gtcctttggg 5281 gtggtccttt gggaaatcac tagcctggct gagcaacctt atcaaggcct gtctaatgaa 5341 caggtgttga agtttgtcat ggatggaggc tatctggatc cccctgataa ctgtccagag 5401 agactcactg acctgatgcg catgtgctgg cagttcaacc ccaagatgag gccaaccttc 5461 ctggaaatcg tcaacctgct caaggatgac ctccacccca gctttccaga agtttccttc 5521 ttctacagcg aggagaacaa ggctcctgag agtgaggagc tggagatgga gtttgaagac 5581 atggagaatg tcccgttgga tcgttcctct cactgtcaga gagaagaggc tgggggccgg 5641 gagggagggt cctcactgag catcaaacgg acctatgatg aacacatccc ctatacccac 5701 atgaatgggg gcaagaagaa cggacgtgtc cttaccctgc caaggtcaaa cccttcctaa 5761 GGATCCtGAT ATCCAGCACA GTGGCGGCCG CTCGAGTCTA GAGGGCCCGT TTAAACCCGC 5821 TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG 5881 CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT 5941 GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC 6001 AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT 6061 TCTGAGGCGG AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC 6121 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 6181 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 6241 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 6301 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 6361 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 6421 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 6481 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT 6541 GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 6601 AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG 6661 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC 6721 CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA 6781 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT 6841 GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA 6901 TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT 6961 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC 7021 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC 7081 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC 7141 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG 7201 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC 7261 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG 7321 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC 7381 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC 7441 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA 7501 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA 7561 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG 7621 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG 7681 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG 7741 GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA 7801 TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG 7861 GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT 7921 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT 7981 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG 8041 TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG 8101 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 8161 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 8221 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 8281 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 8341 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 8401 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 8461 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 8521 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 8581 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 8641 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 8701 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 8761 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 8821 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 8881 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 8941 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 9001 AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 9061 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 9121 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 9181 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 9241 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 9301 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 9361 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 9421 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 9481 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 9541 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG 9601 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT 9661 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT 9721 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT 9781 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 9841 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT 9901 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG 9961 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 10021 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG 10081 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 10141 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 10201 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC 10261 CTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.