pEsrrb-Fluc plasmid

pEsrrb-Fluc plasmid

PVT96699

pEsrrb-Fluc plasmid Information

Plasmid name: pEsrrb(mouse)-Fluc
Alias: -
Gene lenght: 2240bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: Esrrb
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Fluc
Reverse primer:
Copy number: High
Induction:

pEsrrb-Fluc plasmid Description

pEsrrb-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pEsrrb-Fluc plasmid Sequence

1 ggtaccCCCT CCAGATGGTG CCCTGCTATT CCCTCAGGAT ACCTTGTTGG TGGTTAGACA 61 AGGTAGCCAT ACCCAGGAAG ATCACAAGGC ACCAGCCAGA GATGCAGAAA GGATCCAGAG 121 GAGACCCTTA ACTGGGGACC CGCCGCTTAT GATGCAGAGA ATGGGGGGTG GGGGTTCGGA 181 AGGAGAGGAT TCCCAGGGTT AAAGGAACAT TCCATCTAGA ACAGTAGGGA CCTGGGGACC 241 CAGTCTTTAC CAACTACGAA TGTGGGCTTG TAAGCTGGAG TGCTGGGCCT TTAGTGGGCC 301 TTTCATTCCA ATACCTGAAG CTCGCCCTCT CTAGACAAGT TCATGTGCAG GTGAGTGAGT 361 GACACCCTCA GGAGGTGTGC TGAGTGTCAT TGCACTTTAG TGAGACCAAA AGCCCAGTCA 421 CATTACCAAC TCCGTGGTCT GGGTTTGAGG CTAGACCAGG AATTGCTATG TGGGTGCAAT 481 GATAAAGGCT CCGTCCCCAT TCCCTCCGGA GTCACCCTTT TTTGTGGGCA CTTTACTAAA 541 ACGAGGAACT CACAAGCAAG TTACCTGCAA GTGGCACAGG AGGAACAGAC CGCCACCCAC 601 CCCCGCAGCT CTGAGGTCTT CCTTCCTACC CAGGAGTCTT TCTTTCTGTG TTGTTTCCCC 661 TGCTCTTTTC CTTGTTCTGG GTGCCTAAGC CAGTACGGAC TCTCTCCACT GAGGCAGGCC 721 TGGGATCGAG GCTACTTTCA GGGATCATAC CTCTAGACAG TTTTGTTTCT TGGAGACAGG 781 GTCTCTCTTT GCAGTCCTGG CTGTCCTGGG ATACTTTGTG TAGACCAGGC TGGCCCCAAA 841 CTCGAGATCT TTCTGTCTCT GCCTCTGACG TACCACAATA TTTGAGACCA TCTACTTAGG 901 CCTCCTGCCT CAGACCCAAA TCCTAACTGA ACATGTCGAA CAACCAGGTT CCCCTGAACG 961 GTCCTTCAAT CCAGTCCCCT TTACGTGAAC TTGGCTGGAG AGACAGAACT CCCAGTAATG 1021 GGAAAGGCAT GGTTATCCTA CAGTTTTCAG GGGTCCCAGA TGATCTGCCT CATTGGTGAG 1081 GCCCCAAAGC ATTCCAGAAG GTTCTGGGCT AACAAAACAA AACAAGGTGA AAGGATGATA 1141 GTAGGTGATG AGCAGAGTGC TTTTGAGCTT TATGTGAGTT AGGGGTGGGT GGGTGTTTAA 1201 TCTCTAAAAG AAAAAAACAA CTCTGGCTAA GTATTACATG GTGTCATGCC AGGCTTTGAG 1261 GAGCATGCCT TTAGTCCCAG CACTCAGGTG GCAGAGGCAG GCAGATCTCT GTGAGTCTGA 1321 GCCTAGCCTG GTCTACATAG TCAGTTCTAG GCCAGCCAGG GCTACACAGA GCGACTCTGT 1381 AAAGTTACAC GGTGGTGCAG ACCTGTAGTC CCAGTTTGTT CCTGGCTGAG GCAGTAGGAT 1441 ACAGAGTAGG ATGGGGGGGG GGGCTTCATG CGTTCTCATG GAGACAGCCT TTGGGGACTG 1501 GGGGTGCTGG ATGCAAGCTG GATTATCTCA GAGCTAAGAA GGAAAAATAA AAGCCAGAGC 1561 AGATCCCCCC TCCCCGGGGC AGGGGCAGAG ACTGGGGGAT CTCTGAGTTT GAGGCCACTC 1621 TGTTCTACAG AGCAAGATCT TGGTGGCCAG AACTACACAA AGGAACCCTG CCTCGAGAAA 1681 CAAAACAAAA TAAACAAAAA AGCAGAAAAA GAGAAGACAG GATCAGGAAT CATACTTTAG 1741 ATGGGACCGC CATTATCCAG CCCGGACCAC GGGGAAAAAC TCACTTGGTG CCTTTTGGTT 1801 AAACGGTCTC TCTCCGTCCA GTTAGAAAAC TTGGCAGTCT TGGTGACTGT GAAGTCCTAT 1861 TGAAGCAAAT CCCAAGCTTG GACTTCACAG CTCTTTGGGA CAGGCACACG GTCAGCTTCC 1921 ACTTTTTGTG AGGTAGGCAG GTTGGCCAAA TATTTAGGAG GGACAGCTAA CATCTTGCCA 1981 TCCTTAGGCC AGCAATTTGC AAAAGACAGG GTCTCTGATT TGAAGTTTAC GAATTCAAAG 2041 TTTCTTAGAA CTCTGAAGTC GCAGACTTTT CTGGAAAGCA GCTACAGACC TGGCCACCCT 2101 CGCAGCTCTC CTACGTCATA CCCGTAGACA CGCCCATGTG GCCCGCCCAC TAGGCCATAG 2161 CCCCGCCCAA CCTCTCACCT CACTTAGAAC TGACTCCCGC GTTCTGTGTT CGCAGGCTGC 2221 TGAACCGAAT GTCGTCCGAA GACAGGaagc ttggcattcc ggtactgttg gtaaagccac 2281 catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg 2341 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat 2401 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc 2461 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt 2521 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt 2581 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat 2641 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca 2701 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg 2761 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga 2821 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg 2881 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc 2941 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt 3001 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt 3061 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta 3121 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct 3181 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc 3241 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata 3301 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg 3361 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa 3421 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg 3481 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc 3541 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc 3601 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca 3661 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact 3721 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga 3781 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga 3841 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat 3901 aaaggccaag aagggcggaa agatcgccgt gtaattctag agtcggggcg gccggccgct 3961 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 4021 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 4081 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 4141 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga 4201 tccgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg 4261 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg 4321 tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 4381 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 4441 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 4501 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 4561 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 4621 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 4681 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg 4741 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 4801 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 4861 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 4921 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 4981 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 5041 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 5101 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 5161 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 5221 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 5281 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 5341 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 5401 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 5461 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 5521 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 5581 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 5641 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 5701 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 5761 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 5821 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 5881 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 5941 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 6001 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 6061 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 6121 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 6181 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 6241 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt 6301 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 6361 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 6421 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 6481 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 6541 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 6601 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 6661 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt 6721 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 6781 ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg ggaggtactt 6841 ggagcggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg 6901 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa 6961 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctatcga ta

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.