pLV3-CMV-CLDN2-3×FLAG-CopGFP-P

pLV3-CMV-CLDN2-3×FLAG-CopGFP-Puro plasmid

PVT96488

pLV3-CMV-CLDN2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CLDN2(human)-3×FLAG-CopGFP-Puro
Alias: NM_020384.4;claudin-2; OAZON
Gene lenght: 690bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CLDN2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-CLDN2-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CLDN2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cctctcttgg cctccaactt 2341 gtgggctaca tcctaggcct tctggggctt ttgggcacac tggttgccat gctgctcccc 2401 agctggaaaa caagttctta tgtcggtgcc agcattgtga cagcagttgg cttctccaag 2461 ggcctctgga tggaatgtgc cacacacagc acaggcatca cccagtgtga catctatagc 2521 acccttctgg gcctgcccgc tgacatccag gctgcccagg ccatgatggt gacatccagt 2581 gcaatctcct ccctggcctg cattatctct gtggtgggca tgagatgcac agtcttctgc 2641 caggaatccc gagccaaaga cagagtggcg gtagcaggtg gagtcttttt catccttgga 2701 ggcctcctgg gattcattcc tgttgcctgg aatcttcatg ggatcctacg ggacttctac 2761 tcaccactgg tgcctgacag catgaaattt gagattggag aggctcttta cttgggcatt 2821 atttcttccc tgttctccct gatagctgga atcatcctct gcttttcctg ctcatcccag 2881 agaaatcgct ccaactacta cgatgcctac caagcccaac ctcttgccac aaggagctct 2941 ccaaggcctg gtcaacctcc caaagtcaag agtgagttca attcctacag cctgacaggg 3001 tatgtgggat ccGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 3061 AAGGATGACG ATGACAAGta gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 3121 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 3181 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 3241 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 3301 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 3361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 3421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 3481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 3541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 3601 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 3661 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 3721 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 3781 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 3841 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 3901 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 3961 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 4021 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 4081 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 4141 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 4201 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 4261 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 4321 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 4381 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 4441 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 4501 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 4561 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 4621 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 4681 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 4741 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 4801 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 4861 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 4921 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 4981 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 5041 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 5101 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 5161 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 5221 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 5281 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 5341 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 5401 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaATCATCG 5461 TCCTTTCCTt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 5521 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 5581 cggcctcttc cgcgtctTcg ccttcgccct cagacgagtc ggatctccct ttggGccgcc 5641 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 5701 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaGat aagatctgct 5761 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 5821 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 5881 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 5941 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6001 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 6061 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 6121 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctaTCCC GCCCCTAACT 6181 CCGCCCAtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 6241 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 6301 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata 6361 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 6421 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 6481 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 6541 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 6601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 6661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 6721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 6781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 6841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 6901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 6961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 7201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 7501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 7561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 7621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 7681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 7741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 7801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 7861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 7921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 7981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 8041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 8101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 8161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 8221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 8281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 8341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 8461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 8521 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 8581 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 8641 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 8701 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 8761 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 8821 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 8881 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 8941 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.