pLV3-CMV-ITGA10-3×FLAG-CopGFP-

pLV3-CMV-ITGA10-3×FLAG-CopGFP-Puro plasmid

PVT96670

pLV3-CMV-ITGA10-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ITGA10(human)-3×FLAG-CopGFP-Puro
Alias: NM_003637.5;PRO827
Gene lenght: 3501bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ITGA10-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-ITGA10-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ITGA10-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg aactcccctt cgtcactcac 2341 ctgttcttgc ccctggtgtt cctgacaggt ctctgctccc cctttaacct ggatgaacat 2401 cacccacgcc tattcccagg gccaccagaa gctgaatttg gatacagtgt cttacaacat 2461 gttgggggtg gacagcgatg gatgctggtg ggcgccccct gggatgggcc ttcaggcgac 2521 cggagggggg acgtttatcg ctgccctgta gggggggccc acaatgcccc atgtgccaag 2581 ggccacttag gtgactacca actgggaaat tcatctcatc ctgctgtgaa tatgcacctg 2641 gggatgtctc tgttagagac agatggtgat gggggattca tggcctgtgc ccctctctgg 2701 tctcgtgctt gtggcagctc tgtcttcagt tctgggatat gtgcccgtgt ggatgcttca 2761 ttccagcctc agggaagcct ggcacccact gcccaacgct gcccaacata catggatgtt 2821 gtcattgtct tggatggctc caacagcatc tacccctggt ctgaagttca gaccttccta 2881 cgaagactgg tagggaaact gtttattgac ccagaacaga tacaggtggg actggtacag 2941 tatggggaga gccctgtaca tgagtggtcc ctgggagatt tccgaacgaa ggaagaagtg 3001 gtgagagcag caaagaacct cagtcggcgg gagggacgag aaacaaagac tgcccaagca 3061 ataatggtgg cctgcacaga agggttcagt cagtcccatg ggggccgacc cgaggctgcc 3121 aggctactgg tggttgtcac tgatggagag tcccatgatg gagaggagct tcctgcagca 3181 ctaaaggcct gtgaggctgg aagagtgaca cgctatggga ttgcagtcct tggtcactac 3241 ctccggcggc agcgagatcc cagctctttc ctgagagaaa ttagaactat tgccagtgat 3301 ccagatgagc gattcttctt caatgtcaca gatgaggctg ctctgactga cattgtggat 3361 gcactaggag atcggatttt tggccttgaa gggtcccatg cagaaaacga aagctccttt 3421 gggctggaaa tgtctcagat tggtttctcc actcatcggc taaaggatgg gattcttttt 3481 gggatggtgg gggcctatga ctggggaggc tctgtgctat ggcttgaagg aggccaccgc 3541 cttttccccc cacgaatggc actggaagac gagttccccc ctgcattgca gaaccatgca 3601 gcctacctgg gttactctgt ttcttccatg cttttgcggg gtggacgccg cctgtttctc 3661 tctggggctc ctcgatttag acatcgagga aaagtcatcg ccttccagct taagaaagat 3721 ggggctgtga gggttgccca gagcctccag ggggagcaga ttggttcata ctttggcagt 3781 gagctctgcc cattggatac agatagggat ggaacaactg atgtcttact tgtggctgcc 3841 cccatgttcc tgggacccca gaacaaggaa acaggacgtg tttatgtgta tctggtaggc 3901 cagcagtcct tgctgaccct ccaaggaaca cttcagccag aaccccccca ggatgctcgg 3961 tttggctttg ccatgggagc tcttcctgat ctgaaccaag atggttttgc tgatgtggct 4021 gtgggggcgc ctctggaaga tgggcaccag ggagcactgt acctgtacca tggaacccag 4081 agtggagtca ggccccatcc tgcccagagg attgctgctg cctccatgcc acatgccctc 4141 agctactttg gccgaagtgt ggatggtcgg ctagatctgg atggagatga tctggtcgat 4201 gtggctgtgg gtgcccaggg ggcagccatc ctgctcagct cccggcccat tgtccatctg 4261 accccatcac tggaggtgac cccacaggcc atcagtgtgg ttcagaggga ctgtaggcgg 4321 cgaggccaag aggcagtctg tctgactgca gccctttgct tccaagtgac ctcccgtact 4381 cctggtcgct gggatcacca attctacatg aggttcaccg catcactgga tgaatggact 4441 gctggggcac gtgcagcatt tgatggctct ggccagaggt tgtcccctcg gaggctccgg 4501 ctcagtgtgg ggaatgtcac ttgtgagcag ctacacttcc atgtgctgga tacatcagat 4561 tacctccggc cagtggcctt gactgtgacc tttgccttgg acaatactac aaagccaggg 4621 cctgtgctga atgagggctc acccacctct atacaaaagc tggtcccctt ctcaaaggat 4681 tgtggccctg acaatgaatg tgtcacagac ctggtgcttc aagtgaatat ggacatcaga 4741 ggctccagga aggccccatt tgtggttcga ggtggccggc ggaaagtgct ggtatctaca 4801 actctggaga acagaaagga aaatgcttac aatacgagcc tgagtctcat cttctctaga 4861 aacctccacc tggccagtct cactcctcag agagagagcc caataaaggt ggaatgtgcc 4921 gccccttctg ctcatgcccg gctctgcagt gtggggcatc ctgtcttcca gactggagcc 4981 aaggtgacct ttctgctaga gtttgagttt agctgctcct ctctcctgag ccaggtcttc 5041 gtgaagctga ctgccagcag tgacagcctg gagagaaatg ggacccttca agataacaca 5101 gcccagacct cagcctacat ccaatatgag ccccacctcc tgttctctag tgagtctacc 5161 ctgcaccgct atgaggttca cccatatggg accctcccag tgggtcctgg cccagaattc 5221 aaaaccactc tcagggttca gaacctaggc tgctatgtgg tcagtggcct catcatctca 5281 gccctccttc cagctgtggc ccatgggggc aattacttcc tatcactgtc tcaagtcatc 5341 actaacaatg caagctgcat agtgcagaac ctgactgaac ccccaggccc acctgtgcat 5401 ccagaggagc ttcaacacac aaacagactg aatgggagca atactcagtg tcaggtggtg 5461 aggtgccacc ttgggcagct ggcaaagggg actgaggtct ctgttggact attgaggctg 5521 gttcacaatg aatttttccg aagagccaag ttcaagtccc tgacggtggt cagcaccttt 5581 gagctgggaa ccgaagaggg cagtgtccta cagctgactg aagcctcccg ttggagtgag 5641 agcctcttgg aggtggttca gacccggcct atcctcatct ccctgtggat cctcataggc 5701 agtgtcctgg gagggttgct cctgcttgct ctccttgtct tctgcctgtg gaagcttggc 5761 ttctttgccc ataagaaaat ccctgaggaa gaaaaaagag aagagaagtt ggagcaagga 5821 tccGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC 5881 GATGACAAGt agcggccgcG aaggatctgc gatcgctccg gtgcccgtca gtgggcagag 5941 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc 6001 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 6061 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 6121 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc 6181 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc 6241 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga 6301 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt 6361 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac 6421 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc 6481 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag 6541 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc 6601 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc 6661 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac 6721 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc 6781 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc 6841 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag 6901 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg 6961 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc 7021 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg 7081 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc 7141 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc 7201 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat 7261 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 7321 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 7381 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 7441 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 7501 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 7561 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 7621 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 7681 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 7741 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 7801 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 7861 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 7921 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 7981 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 8041 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 8101 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 8161 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 8221 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaATCATC GTCCTTTCCT 8281 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 8341 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 8401 ccgcgtctTc gccttcgccc tcagacgagt cggatctccc tttggGccgc ctccccgcct 8461 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 8521 aaagggggga ctggaagggc taattcactc ccaacgaaGa taagatctgc tttttgcttg 8581 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 8641 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 8701 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 8761 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 8821 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 8881 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 8941 actcatcaat gtatcttatc atgtctggct ctagctaTCC CGCCCCTAAC TCCGCCCAtc 9001 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 9061 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 9121 ttttttggag gcctagactt ttgcagagac caaattcgta atcatgtcat agctgtttcc 9181 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 9241 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 9301 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 9361 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 9421 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 9481 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 9541 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 9601 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 9661 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 9721 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 9781 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 9841 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 9901 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 9961 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 10021 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 10081 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 10141 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 10201 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 10261 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 10321 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 10381 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 10441 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 10501 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 10561 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 10621 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 10681 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 10741 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 10801 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 10861 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 10921 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 10981 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 11041 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 11101 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 11161 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 11221 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 11281 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 11341 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 11401 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 11461 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 11521 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 11581 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 11641 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 11701 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 11761 acgttgtaaa acgacggcca gtgccaagct g

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.