pCMV-3×FLAG-KMT5B-Neo plasmid

pCMV-3×FLAG-KMT5B-Neo plasmid

PVT93954

pCMV-3×FLAG-KMT5B-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-KMT5B(human)-Neo
Alias: NM_017635.5;CGI-85; CGI85; MRD51; SUV420H1
Gene lenght: 2655bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-KMT5B-Neo plasmid Description

pCMV-3×FLAG-KMT5B-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-KMT5B-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atgaagtggt tgggagaatc caagaacatg 1021 gtggtgaatg gcaggagaaa tggaggcaag ttgtctaatg accatcagca gaatcaatca 1081 aaattacagc acacggggaa ggacaccctg aaggctggca aaaatgcagt cgagaggagg 1141 tcgaacagat gtaatggtaa ctcgggattt gaaggacaga gtcgctatgt accatcctct 1201 ggaatgtccg ccaaggaact ctgtgaaaat gatgacctag caaccagttt ggttcttgat 1261 ccctatttag gttttcaaac acacaaaatg aatactagcg cctttccttc gaggagctca 1321 aggcattttt caaaatctga cagtttttct cacaacaacc ctgtgagatt taggcctatt 1381 aaaggaaggc aggaagaact aaaggaagta attgaacgtt ttaagaaaga tgaacacttg 1441 gagaaagcct tcaaatgttt gacttcaggc gaatgggcac ggcactattt tctcaacaag 1501 aataaaatgc aggagaaatt attcaaagaa catgtattta tttatttgcg aatgtttgca 1561 actgacagtg gatttgaaat attgccatgt aatagatact catcagaaca aaatggagcc 1621 aaaatagttg caacaaaaga gtggaaacga aatgacaaaa tagaattact ggtgggttgt 1681 attgccgaac tttcagaaat tgaggagaac atgctactta gacatggaga aaacgacttc 1741 agtgtcatgt actccacaag gaaaaactgt gctcaactct ggctgggtcc tgctgcgttt 1801 ataaaccatg attgcagacc taattgtaag tttgtgtcaa ctggtcgaga tacagcatgt 1861 gtgaaggctc taagagacat tgaacctgga gaagaaattt cttgttatta tggagatggg 1921 ttctttggag aaaataatga gttctgcgag tgttacactt gcgaaagacg gggcactggt 1981 gcttttaaat ccagagtggg actgcctgcg cctgctcctg ttatcaatag caaatatgga 2041 ctcagagaaa cagataaacg tttaaatagg cttaaaaagt taggtgacag cagcaaaaat 2101 tcagacagtc aatctgtcag ctctaacact gatgcagata ccactcagga aaaaaacaat 2161 gcaacttcta accgaaaatc ttcagttggc gtaaaaaaga atagcaagag cagaacgtta 2221 acgaggcaat ctatgtcaag aattccagct tcttccaact ctacctcatc taagctaact 2281 catataaata attccagggt accaaagaaa ctgaagaagc ctgcaaagcc tttactttca 2341 aagataaaat tgagaaatca ttgcaagcgg ctggagcaaa agaatgcttc aagaaaactc 2401 gaaatgggaa acttagtact gaaagagcct aaagtagttc tgtataaaaa tttgcccatt 2461 aaaaaagata aggagccaga gggaccagcc caagccgcag ttgccagcgg gtgcttgact 2521 agacacgcgg cgagagaaca cagacagaat cctgtgagag gtgctcattc gcagggggag 2581 agctcgccct gcacctacat aactcggcgg tcagtgagga caagaacaaa tctgaaggag 2641 gcctctgaca tcaagcttga accaaatacg ttgaatggct ataaaagcag tgtgacggaa 2701 ccttgccccg acagtggtga acagctgcag ccagctcctg tgctgcagga ggaagaactg 2761 gctcatgaga ctgcacaaaa aggggaggca aagtgtcata agagtgacac aggcatgtcc 2821 aaaaagaagt cacgacaagg aaaacttgtg aaacagtttg caaaaataga ggaatctact 2881 ccagtgcacg attctcctgg aaaagacgac gcggtaccag atttgatggg tccccattct 2941 gaccagggtg agcacagtgg cactgtgggc gtgcctgtga gctacacaga ctgtgctcct 3001 tcacccgtcg gttgttcagt tgtgacatca gatagcttca aaacaaaaga cagctttaga 3061 actgcaaaaa gtaaaaagaa gaggcgaatc acaaggtatg atgcacagtt aatcctagaa 3121 aataactctg ggattcccaa attgactctt cgtaggcgtc atgatagcag cagcaaaaca 3181 aatgaccaag agaatgatgg aatgaactct tccaaaataa gcatcaagtt aagcaaagac 3241 catgacaacg ataacaatct ctatgtagca aagcttaata atggatttaa ctcaggatca 3301 ggcagtagtt ctacaaaatt aaaaatccag ctaaaacgag atgaggaaaa tagggggtct 3361 tatacagagg ggcttcatga aaatggggtg tgctgcagtg atcctctttc tctcttggag 3421 tctcgaatgg aggtggatga ctatagtcag tatgaggaag aaagtacaga tgattcctcc 3481 tcttctgagg gcgatgaaga ggaggatgac tatgatgatg actttgaaga cgattttatt 3541 cctcttcctc cagctaagcg cttgaggtta atagttggaa aagactctat agatattgac 3601 atttcttcaa ggagaagaga agatcagtct ttaaggctta atgcctaaga attctgcaga 3661 tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc 3721 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 3781 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 3841 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 3901 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg 3961 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc 4021 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 4081 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 4141 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 4201 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 4261 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 4321 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 4381 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt 4441 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 4501 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 4561 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 4621 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 4681 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 4741 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 4801 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 4861 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 4921 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 4981 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 5041 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 5101 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 5161 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 5221 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 5281 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 5341 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg 5401 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 5461 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 5521 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 5581 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 5641 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 5701 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 5761 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 5821 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 5881 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 5941 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 6001 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 6061 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 6121 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 6181 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 6241 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 6301 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 6361 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 6421 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 6481 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 6541 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 6601 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 6661 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 6721 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 6781 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 6841 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 6901 tggtagcggt GGTttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 6961 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 7021 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 7081 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 7141 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 7201 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 7261 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 7321 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 7381 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 7441 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 7501 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 7561 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 7621 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 7681 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 7741 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 7801 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 7861 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 7921 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 7981 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 8041 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 8101 atttccccga aaagtgccac ctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.