pCMV-mCherry-Oasl1-3×FLAG-Neo

pCMV-mCherry-Oasl1-3×FLAG-Neo plasmid

PVT96658

pCMV-mCherry-Oasl1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-mCherry-Oasl1(mouse)-3×FLAG-Neo
Alias: NM_001359945.1;7530414C13Rik; oasl9
Gene lenght: 1533bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: mCherry-F1
Reverse primer:
Copy number: High
Induction:

pCMV-mCherry-Oasl1-3×FLAG-Neo plasmid Description

pCMV-mCherry-Oasl1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-mCherry-Oasl1-3×FLAG-Neo plasmid Sequence

1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct 661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta 721 aacttaagct tggtaccgcc accatggtga gcaagggcga ggaggataac atggccatca 781 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg 841 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 901 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg 961 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 1021 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 1081 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 1141 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg 1201 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga 1261 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc 1321 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact 1381 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg 1441 agctgtacaa gtggatcgga ggagggggaa gcggtggagg cggtagtgag ctcggatcca 1501 tggcagtcgc ccaggagctg tacggcttcc cggcctccaa gctggactcc tttgtggctc 1561 agtggctgca gccaaccaga gagtggaaag aagaggtcct ggagaccgtg cagacagtgg 1621 agcagttcct gaggcaggag aatttccgtg aagatcgtgg cccggctcgg gatgtgcgcg 1681 tgctcaaggt actcaaggta ggctgctttg ggaatggcac cgtgctcagg agcactacag 1741 acgtggagct ggtcgtgttc ctgagctgtt tccacagctt ccaggaagaa gccaagcacc 1801 atcaggctgt cctgagactg atacagaaaa ggatgtacta ctgccaggag ctgatggacc 1861 ttgggctcag taacctgagt gtgactaaca gagtacccag tagtctcatc ttcacgatcc 1921 agaccaggga gacctgggag accatcactg tcaccgttgt gcccgcctac agagccctgg 1981 gcccttcctg tcccagctcc gaggtctacg caaatctgat caaggctaat gggtacccag 2041 gaaatttctc tccatccttc agcgagctgc agcgaaactt cgtgaagcat cggccgacga 2101 agctgaagag cctccttcgg ttggtcaaac actggtacca gcagtatgtg agagacaagt 2161 gcccccgggc caacctgccc cctctctatg ccctggagct gctcactgtc tatgcctggg 2221 aagcgggcac ccgggaggat gccaacttca ggctggatga aggcctcgcc acggtgatgg 2281 agctgctcca ggatcatgag ctcctctgta tctactggac caagcactac acgctgcagc 2341 acccggtcat cgaggcctgt gtcaggagac agctcagggg acaaaggcct atcatcctgg 2401 acccagcaga ccccaccaac aatgtggcag aaggctacag atgggacata gtggctcagc 2461 gggccaacca gtgtctgaaa caggactgtt gctatgacaa cagggacagc cccgtcccca 2521 gctggagggt gaagagagca cccgatatcc aggtgaccgt gcaggagtgg gggcactcgg 2581 atttaacctt ctgggtgaac ccttatgaac ccataaagaa actgaaagag aaaatccaac 2641 tgagccaggg ctacttgggc ctgcagcgtc tgtcctttca ggagcccggc ggagaacgtc 2701 agctcatcag aagccattgc acgctcgcct actacggaat cttctgcgac acccacatct 2761 gcctgctgga caccatctcc cctgagatcc aggtctttgt gaaaaacccg gatggcagga 2821 gccacgccta tgcgatccac ccgcttgatt acgtcctgaa cctgaagcag cagatagaag 2881 acaggcaggg ccttcgatgc caggagcagc gcctggagtt ccagggccac atcctggagg 2941 actggtttga ctttaaatcc tatggcatcc aagacagtgt cacagtcatc ctgtccaaga 3001 cgacggaggg ggcagctcca tttgtgccca gcctcgagga ctacaaagac catgacggtg 3061 attataaaga tcatgacatc gactacaagg atgacgatga caagtagtga gggcccgata 3121 tctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 3181 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 3241 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 3301 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 3361 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 3421 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 3481 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 3541 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 3601 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 3661 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 3721 aaacggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg 3781 acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg 3841 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata 3901 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct 3961 cgcagccaac gtcggggcgg caggccctgc catagcagat ctgcgcagct ggggctctag 4021 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 4081 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 4141 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg 4201 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 4261 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 4321 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 4381 ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta 4441 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 4501 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 4561 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 4621 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 4681 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 4741 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 4801 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga 4861 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 4921 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 4981 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 5041 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 5101 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 5161 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 5221 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 5281 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 5341 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc 5401 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 5461 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 5521 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 5581 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 5641 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc 5701 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg 5761 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 5821 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 5881 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 5941 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 6001 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 6061 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 6121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 6181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 6241 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 6301 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 6361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 6421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 6481 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 6541 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 6601 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 6661 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 6721 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 6781 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 6841 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 6901 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 6961 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 7021 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 7081 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 7141 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 7201 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 7261 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 7321 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 7381 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 7441 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 7501 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 7561 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 7621 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 7681 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 7741 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 7801 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 7861 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 7921 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 7981 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 8041 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 8101 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 8161 tgacgtcgac ggatcgggag atctcccgat cccctatggt cgactctcag tacaatctgc 8221 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag 8281 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag 8341 aatctgctta gg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.