pCMV-Tlr9-3×FLAG-Neo plasmid

pCMV-Tlr9-3×FLAG-Neo plasmid

PVT96745

pCMV-Tlr9-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Tlr9(mouse)-3×FLAG-Neo
Alias: NM_031178.2
Gene lenght: 3096bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Tlr9-3×FLAG-Neo plasmid Description

pCMV-Tlr9-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Tlr9-3×FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCGCC 961 ACCatggttc tccgtcgaag gactctgcac cccttgtccc tcctggtaca ggctgcagtg 1021 ctggctgaga ctctggccct gggtaccctg cctgccttcc taccctgtga gctgaagcct 1081 catggcctgg tggactgcaa ttggctgttc ctgaagtctg taccccgttt ctctgcggca 1141 gcatcctgct ccaacatcac ccgcctctcc ttgatctcca accgtatcca ccacctgcac 1201 aactctgact tcgtccacct gtccaacctg cggcagctga acctcaagtg gaactgtcca 1261 cccactggcc ttagccccct gcacttctct tgccacatga ccattgagcc cagaaccttc 1321 ctggctatgc gtacactgga ggagctgaac ttgagctata atggtatcac cactgtgccc 1381 cgactgccca gctccctggt gaatctgagc ctgagccaca ccaacatcct ggttctagat 1441 gctaacagcc tcgccggcct atacagcctg cgcgttctct tcatggacgg gaactgctac 1501 tacaagaacc cctgcacagg agcggtgaag gtgaccccag gcgccctcct gggcctgagc 1561 aatctcaccc atctgtctct gaagtataac aacctcacaa aggtgccccg ccaactgccc 1621 cccagcctgg agtacctcct ggtgtcctat aacctcattg tcaagctggg gcctgaagac 1681 ctggccaatc tgacctccct tcgagtactt gatgtgggtg ggaattgccg tcgctgtgac 1741 catgccccca atccctgtat agaatgtggc caaaagtccc tccacctgca ccctgagacc 1801 ttccatcacc tgagccatct ggaaggcctg gtgctgaagg acagctctct ccatacactg 1861 aactcttcct ggttccaagg tctggtcaac ctctcggtgc tggacctaag cgagaacttt 1921 ctctatgaaa gcatcaccca caccaatgcc tttcagaacc taacccgcct gcgcaagctc 1981 aacctgtcct tcaattaccg caagaaggta tcctttgccc gcctccacct ggcaagttcc 2041 tttaagaacc tggtgtcact gcaggagctg aacatgaacg gcatcttctt ccgcttgctc 2101 aacaagtaca cgctcagatg gctggccgat ctgcccaaac tccacactct gcatcttcaa 2161 atgaacttca tcaaccaggc acagctcagc atctttggta ccttccgagc ccttcgcttt 2221 gtggacttgt cagacaatcg catcagtggg ccttcaacgc tgtcagaagc cacccctgaa 2281 gaggcagatg atgcagagca ggaggagctg ttgtctgcgg atcctcaccc agctccgctg 2341 agcacccctg cttctaagaa cttcatggac aggtgtaaga acttcaagtt caccatggac 2401 ctgtctcgga acaacctggt gactatcaag ccagagatgt ttgtcaatct ctcacgcctc 2461 cagtgtctta gcctgagcca caactccatt gcacaggctg tcaatggctc tcagttcctg 2521 ccgctgacta atctgcaggt gctggacctg tcccataaca aactggactt gtaccactgg 2581 aaatcgttca gtgagctacc acagttgcag gccctggacc tgagctacaa cagccagccc 2641 tttagcatga agggtatagg ccacaatttc agttttgtga cccatctgtc catgctacag 2701 agccttagcc tggcacacaa tgacattcat acccgtgtgt cctcacatct caacagcaac 2761 tcagtgaggt ttcttgactt cagcggcaac ggtatgggcc gcatgtggga tgaggggggc 2821 ctttatctcc atttcttcca aggcctgagt ggcctgctga agctggacct gtctcaaaat 2881 aacctgcata tcctccggcc ccagaacctt gacaacctcc ccaagagcct gaagctgctg 2941 agcctccgag acaactacct atctttcttt aactggacca gtctgtcctt cctacccaac 3001 ctggaagtcc tagacctggc aggcaaccag ctaaaggccc tgaccaatgg caccctgcct 3061 aatggcaccc tcctccagaa actcgatgtc agtagcaaca gtatcgtctc tgtggtccca 3121 gccttcttcg ctctggcggt cgagctgaaa gaggtcaacc tcagccacaa cattctcaag 3181 acggtggatc gctcctggtt tgggcccatt gtgatgaacc tgacagttct agacgtgaga 3241 agcaaccctc tgcactgtgc ctgtggggca gccttcgtag acttactgtt ggaggtgcag 3301 accaaggtgc ctggcctggc taatggtgtg aagtgtggca gccccggcca gctgcagggc 3361 cgtagcatct tcgcgcagga cctgcggctg tgcctggatg aggtcctctc ttgggactgc 3421 tttggccttt cactcttggc tgtggccgtg ggcatggtgg tgcctatact gcaccatctc 3481 tgcggctggg acgtctggta ctgttttcat ctgtgcctgg catggctacc tttgctagcc 3541 cgcagccgac gcagcgccca aactctccct tatgatgcct tcgtggtgtt cgataaggca 3601 cagagcgcag ttgccgactg ggtgtataac gagctgcggg tgcggctgga ggagcggcgc 3661 ggccgccgag ccctacgctt gtgtctggag gaccgagatt ggctgcctgg ccagacgctc 3721 ttcgagaacc tctgggcttc catctatggg agccgcaaga ctctatttgt gctggcccac 3781 acggaccgcg tcagtggcct cctgcgcacc agcttcctgc tggctcagca gcgcctgttg 3841 gaagaccgca aggacgtggt ggtgttggtg atcctgcgtc cggatgccca ccgctcccgc 3901 tatgtgcgac tgcgccagcg tctctgccgc cagagtgtgc tcttctggcc ccagcagccc 3961 aacgggcagg ggggcttctg ggcccagctg agtacagccc tgactaggga caaccgccac 4021 ttctataacc agaacttctg ccggggacct acagcagaaC TCGAGGACTA CAAAGACCAT 4081 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GTAGTGAGGG 4141 CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT 4201 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA 4261 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG 4321 GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG 4381 GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC 4441 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 4501 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 4561 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 4621 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 4681 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 4741 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 4801 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 4861 GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG 4921 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC 4981 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG 5041 TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC 5101 CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC 5161 TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG 5221 GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA 5281 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT 5341 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC 5401 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG 5461 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG 5521 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC 5581 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC 5641 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG 5701 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC 5761 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG 5821 AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC 5881 GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG 5941 CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG 6001 TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG 6061 AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC 6121 ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT 6181 CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA 6241 CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT 6301 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT 6361 ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA 6421 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG 6481 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG 6541 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA 6601 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 6661 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 6721 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 6781 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 6841 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 6901 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 6961 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 7021 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 7081 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 7141 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 7201 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 7261 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 7321 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC 7381 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 7441 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 7501 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 7561 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 7621 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 7681 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 7741 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 7801 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 7861 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 7921 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 7981 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 8041 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 8101 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 8161 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 8221 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 8281 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 8341 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 8401 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 8461 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 8521 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.