pCMV-DOCK1-3×FLAG-Neo plasmid

pCMV-DOCK1-3×FLAG-Neo plasmid

PVT96641

pCMV-DOCK1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-DOCK1(human)-3×FLAG-Neo
Alias: NM_001380;ced5; DOCK180
Gene lenght: 5595bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-DOCK1-3×FLAG-Neo plasmid Description

pCMV-DOCK1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-DOCK1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgac gcgctgggtg cccaccaagc 781 gcgaggagaa gtacggcgtg gctttttata actatgatgc cagaggagcg gatgaacttt 841 ctttacagat cggagacact gtgcacatct tagaaacata tgaagggtgg taccgaggtt 901 acacgttacg aaaaaagtct aagaagggta tatttcctgc ttcatatatt catcttaaag 961 aagcgatagt tgaaggaaaa gggcaacatg aaacagtcat cccgggtgac ctccccctca 1021 tccaggaagt caccacgaca ctccgagagt ggtccaccat ctggaggcag ctctacgtgc 1081 aagataacag ggagatgttt cgaagtgtgc ggcacatgat ctatgacctt attgaatggc 1141 gatcacaaat tctttctgga actctgcctc aggatgaact caaagaactg aagaagaagg 1201 tcacagccaa aattgattat ggaaacagaa ttctagattt ggacctggtg gttagagatg 1261 aagatgggaa tattttggat ccagaattaa ctagcacgat tagtctcttc agagctcatg 1321 aaatagcttc taaacaagtg gaggaaaggt tacaagagga aaaatctcaa aagcagaaca 1381 tagatattaa cagacaagcc aagtttgctg caaccccttc tctggccttg tttgtgaacc 1441 tcaaaaatgt ggtttgtaaa ataggagaag atgctgaagt cctcatgtct ctatatgacc 1501 ctgtggagtc caaattcatc agtgagaact acctggttcg ctggtccagt tcaggattac 1561 ctaaagacat agacagatta cataatttgc gagccgtgtt tactgacctc ggaagcaaag 1621 acctgaaaag ggagaaaatc agttttgtct gtcagattgt tcgcgtgggt cgcatggagc 1681 tgagggacaa caacaccagg aaactgacct cggggttgcg gcgacctttt ggagtggctg 1741 tgatggatgt aacagatata ataaatggaa aagtagatga tgaagataag cagcatttca 1801 ttccctttca gccggtggca ggggagaatg acttccttca gactgttata aacaaagtca 1861 tcgctgccaa agaagtcaac cacaaggggc agggtttgtg ggtaacattg aaattacttc 1921 ctggagatat ccatcagatc cgaaaagagt ttccgcattt agtggacagg accacagctg 1981 tggctcgaaa aacagggttt ccggagataa tcatgcctgg tgatgttcga aatgatatct 2041 atgtaacatt agttcaagga gattttgata aaggaagcaa aacaacagcg aagaacgtgg 2101 aggtcacggt gtctgtgtac gatgaggatg ggaaacgatt agagcatgtg attttcccgg 2161 gtgctggtga tgaagcgatt tcagagtaca aatctgtgat ttactaccaa gtaaagcagc 2221 cacgctggtt tgagactgtt aaggtggcca ttcccatcga ggacgttaac cgcagtcacc 2281 ttcggtttac cttccgccac aggtcatcac aggactctaa ggataaatct gagaaaatat 2341 ttgcactagc atttgtcaag ctgatgagat acgatggtac caccctgcga gacggagagc 2401 acgatcttat cgtctataag gccgaagcaa agaagctgga agatgctgcc acgtacttga 2461 gtctgccctc cacgaaggca gagttggaag aaaagggcca ctcggccacc ggcaagagca 2521 tgcagagcct tgggagctgc accattagca aggactcctt ccagatctcc acgctcgtgt 2581 gctccaccaa actgactcag aacgtggacc ttctggggct cttgaaatgg cgctccaaca 2641 ccagcctgct gcagcagaac ttgaggcagc tgatgaaagt cgatggtggt gaagtagtga 2701 agtttcttca ggacacgttg gatgccctct tcaacatcat gatggagaac tcagagagtg 2761 agacttttga cacgttagtc tttgatgctc tggtatttat cattggactg attgctgata 2821 gaaaatttca gcattttaat cctgttttgg aaacttacat taagaaacac tttagtgcaa 2881 cgttagccta cacgaagttg acaaaagtgt tgaagaacta cgtggacggt gctgagaagc 2941 cgggagtaaa tgagcagctg tacaaagcca tgaaagcgct agaatccatc ttcaagttca 3001 tcgtgcgctc caggatcctg ttcaatcaac tgtatgaaaa caagggagag gctgacttcg 3061 tggaatcttt gctgcagctc ttcaggtcca tcaatgacat gatgagcagc atgtcagacc 3121 agaccgtccg ggtgaagggg gcagcactga aatacttacc aacgatcgtc aacgatgtga 3181 aattggtgtt tgatcccaaa gagctcagca aaatgtttac tgaattcatc ctcaatgttc 3241 ccatgggctt gctgaccatc cagaaactct actgcttgat cgaaatcgtc cacagtgacc 3301 tcttcacaca gcatgactgc agagagatcc tgcttcccat gatgaccgat cagctcaagt 3361 accatctgga gagacaggag gacctggagg cctgctgtca gctgctcagc cacatcctgg 3421 aggtgctgta caggaaggac gtggggccaa cccagaggca cgtccagatt atcatggaga 3481 aacttctccg gaccgtgaac cgaaccgtca tttccatggg acgagattct gaactcattg 3541 gaaacttcgt ggcttgcatg acagctattt tacgacaaat ggaagattac cattatgccc 3601 acttgatcaa gacttttggg aaaatgagga ctgatgtggt agatttccta atggaaacat 3661 tcatcatgtt taagaacctc attggaaaga acgtttaccc cttcgactgg gtgatcatga 3721 acatggtgca aaataaagtc ttcctgcgag caattaatca gtatgcagat atgctgaaca 3781 aaaaatttct ggatcaagcc aactttgagc tacagctgtg gaacaactac tttcacctgg 3841 ctgttgcttt ccttactcaa gagtccctgc aactggagaa tttttcaagt gccaagagag 3901 ccaaaatcct taacaagtac ggagatatga ggagacagat tggctttgaa atcagagaca 3961 tgtggtacaa ccttggtcaa cacaagataa agttcattcc agaaatggtg ggcccaatat 4021 tagaaatgac attaattccc gagacggagc tgcgcaaagc caccatcccc atcttctttg 4081 atatgatgca gtgtgaattc cattcgaccc gaagcttcca aatgtttgaa aatgagatca 4141 tcaccaagct ggatcatgaa gtcgaaggag gcagaggaga cgaacagtac aaagtgttat 4201 ttgataaaat ccttctggaa cactgcagga agcacaaata cctcgccaaa acaggagaaa 4261 cttttgtaaa actcgttgtg cgcttaatgg aaaggctttt ggattataga accatcatgc 4321 acgacgagaa caaagaaaac cgcatgagct gcaccgtcaa tgtgctgaat ttctacaaag 4381 aaattgaaag agaagaaatg tatataaggt atttgtacaa gctctgtgac ctgcacaagg 4441 agtgtgataa ctacaccgaa gcggcttaca ccttgcttct ccatgcaaag cttcttaagt 4501 ggtcggagga tgtgtgtgtg gcccacctca cccagcggga cgggtaccag gccaccacgc 4561 agggacagct gaaggagcag ctctaccagg aaatcatcca ctacttcgac aaaggcaaga 4621 tgtgggagga ggccattgcc ttgggcaagg agctagccga gcagtatgag aacgaaatgt 4681 ttgattatga gcaactcagc gaattgctga aaaaacaggc tcagttttat gaaaacatcg 4741 tcaaagtgat caggcccaag cctgactatt ttgctgttgg ctactacgga caagggttcc 4801 ccacattcct gcggggaaaa gttttcattt accgagggaa agagtatgag cgccgggaag 4861 attttgaggc tcggctctta actcagtttc caaacgccga gaaaatgaag acaacatctc 4921 caccaggcga cgatattaaa aactctcctg gccagtatat tcagtgcttc acagtgaagc 4981 ccaaactcga tctgcctcct aagtttcaca ggccagtgtc agagcagatt gtaagttttt 5041 acagggtgaa cgaggtccag cgatttgaat attctcggcc aatccggaag ggagagaaaa 5101 acccagacaa tgaatttgcg aatatgtgga tcgagagaac catatataca actgcatata 5161 aattacctgg aattttaagg tggtttgagg tcaagtctgt tttcatggtg gaaatcagcc 5221 ccctggagaa tgccattgag accatgcagc tgacgaacga caagatcaac agcatggtgc 5281 agcagcacct ggatgacccc agcctgccca tcaacccgct ctccatgctc ctgaacggca 5341 tcgtggaccc agctgtcatg gggggcttcg caaactacga aaaggccttc tttacagacc 5401 ggtacctgca ggagcaccct gaggcccatg aaaagatcga gaagctcaag gacctgattg 5461 cttggcagat tccttttctg gccgaaggga tcagaatcca tggagacaaa gtcacggagg 5521 cactgaggcc gttccacgag aggatggagg cctgtttcaa acagctgaag gaaaaggtgg 5581 agaaagagta cggcgtccga atcatgccct caagtctgga tgatagaaga ggcagccgcc 5641 cccggtccat ggtgcggtcc ttcacgatgc cttcctcatc ccgccctctg tctgtggcct 5701 ctgtctcttc cctctcatcg gacagcaccc cttccagacc aggctccgac gggtttgccc 5761 tggagcctct cctgccaaag aaaatgcact ccaggtccca ggacaagctg gacaaggatg 5821 acctggagaa ggagaagaag gacaagaaga aggaaaaaag gaacagcaaa catcaagaga 5881 tatttgagaa agaatttaaa cccaccgaca tttccctgca gcagtctgag gctgtgatcc 5941 tttcggaaac gataagtccc ctgcggcccc agagaccgaa gagccaggtg atgaacgtca 6001 ttggaagcga aaggcgcttc tcggtgtccc cctcgtcacc gtcctcccag caaacacccc 6061 ctccagttac accaagggcc aagctcagct tcagcatgca gtcgagcttg gagctgaacg 6121 gcatgacggg ggcggacgtg gccgatgtcc caccccctct gcctctcaaa ggcagcgtgg 6181 cagattacgg gaatttgatg gaaaaccagg acttgctggg ctcgccaaca cctccacctc 6241 cccctccaca ccagaggcat ctgccacctc cactgcccag caaaactccg cctcctcccc 6301 ctccaaagac aactcgcaag caggcatcgg tggactccgg gatcgtgcag GGATCCGACT 6361 ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA 6421 AGTAGTGAGG GCCCGATATC TCGACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT 6481 GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC 6541 TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG 6601 GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC 6661 TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC 6721 CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC 6781 CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA 6841 GCTGACGTCC TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC 6901 CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC 6961 GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG 7021 GGCCGCCTCC CCGCCTGGAA ACGGGGGAGG CTAACTGAAA CACGGAAGGA GACAATACCG 7081 GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG GTGTTGGGTC 7141 GTTTGTTCAT AAACGCGGGG TTCGGTCCCA GGGCTGGCAC TCTGTCGATA CCCCACCGAG 7201 ACCCCATTGG GGCCAATACG CCCGCGTTTC TTCCTTTTCC CCACCCCACC CCCCAAGTTC 7261 GGGTGAAGGC CCAGGGCTCG CAGCCAACGT CGGGGCGGCA GGCCCTGCCA TAGCAGATCT 7321 GCGCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC 7381 GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC 7441 TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA 7501 TCGGGGCATC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT 7561 TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT 7621 GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA 7681 CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG GGGATTTCGG CCTATTGGTT 7741 AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG 7801 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 7861 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 7921 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 7981 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 8041 GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 8101 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 8161 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 8221 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 8281 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 8341 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 8401 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 8461 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 8521 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 8581 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 8641 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 8701 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 8761 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 8821 CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 8881 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 8941 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 9001 CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC 9061 CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA 9121 GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA 9181 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA 9241 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC 9301 CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC 9361 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA 9421 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 9481 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 9541 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 9601 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 9661 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 9721 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 9781 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 9841 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 9901 TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 9961 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 10021 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 10081 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 10141 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA 10201 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 10261 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 10321 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 10381 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 10441 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 10501 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 10561 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 10621 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 10681 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 10741 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 10801 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 10861 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 10921 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 10981 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 11041 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 11101 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 11161 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 11221 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 11281 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 11341 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 11401 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 11461 TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT CTCCCGATCC CCTATGGTCG 11521 ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATCTGCT CCCTGCTTGT 11581 GTGTTGGAGG TCGCTGAGTA GTGCGCGAGC AAAATTTAAG CTACAACAAG GCAAGGCTTG 11641 ACCGACAATT GCATGAAGAA TCTGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.