pCMV-C1R-3×FLAG-Neo plasmid

pCMV-C1R-3×FLAG-Neo plasmid

PVT94511

pCMV-C1R-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-C1R(human)-3×FLAG-Neo
Alias: NM_001733.7;EDS8; EDSPD1
Gene lenght: 2115bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-C1R-3×FLAG-Neo plasmid Description

pCMV-C1R-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-C1R-3×FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCgcc 961 accATGTGGC TCTTGTACCT CCTGGTGCCG GCCCTGTTCT GCAGGGCAGG AGGCTCCATT 1021 CCCATCCCTC AGAAGTTATT TGGGGAGGTG ACTTCCCCTC TGTTCCCCAA GCCTTACCCC 1081 AACAACTTTG AAACAACCAC TGTGATCACA GTCCCCACGG GATACAGGGT GAAGCTCGTC 1141 TTCCAGCAGT TTGACCTGGA GCCTTCTGAA GGCTGCTTCT ATGATTATGT CAAGATCTCT 1201 GCTGATAAGA AAAGCCTGGG GAGGTTCTGT GGGCAACTGG GTTCTCCACT GGGCAACCCC 1261 CCGGGAAAGA AGGAATTTAT GTCCCAAGGG AACAAGATGC TGCTGACCTT CCACACAGAC 1321 TTCTCCAACG AGGAGAATGG GACCATCATG TTCTACAAGG GCTTCCTGGC CTACTACCAA 1381 GCTGTGGACC TTGATGAATG TGCTTCCCGG AGCAAATCAG GGGAGGAGGA TCCCCAGCCC 1441 CAGTGCCAGC ACCTGTGTCA CAACTACGTT GGAGGCTACT TCTGTTCCTG CCGTCCAGGC 1501 TATGAGCTTC AGGAAGACAG GCATTCCTGC CAGGCTGAGT GCAGCAGCGA GCTGTACACG 1561 GAGGCATCAG GCTACATCTC CAGCCTGGAG TACCCTCGGT CCTACCCCCC TGACCTGCGC 1621 TGCAACTACA GCATCCGGGT GGAGCGGGGC CTCACCCTGC ACCTCAAGTT CCTGGAGCCT 1681 TTTGATATTG ATGACCACCA GCAAGTACAC TGCCCCTATG ACCAGCTACA GATCTATGCC 1741 AACGGGAAGA ACATTGGCGA GTTCTGTGGG AAGCAAAGGC CCCCCGACCT CGACACCAGC 1801 AGCAATGCTG TGGATCTGCT GTTCTTCACA GATGAGTCGG GGGACAGCCG GGGCTGGAAG 1861 CTGCGCTACA CCACCGAGAT CATCAAGTGC CCCCAGCCCA AGACCCTAGA CGAGTTCACC 1921 ATCATCCAGA ACCTGCAGCC TCAGTACCAG TTCCGTGACT ACTTCATTGC TACCTGCAAG 1981 CAAGGCTACC AGCTCATAGA GGGGAACCAG GTGCTGCATT CCTTCACAGC TGTCTGCCAG 2041 GATGATGGCA CGTGGCATCG TGCCATGCCC AGATGCAAGA TCAAGGACTG TGGGCAGCCC 2101 CGAAACCTGC CTAATGGTGA CTTCCGTTAC ACCACCACAA TGGGAGTGAA CACCTACAAG 2161 GCCCGTATCC AGTACTACTG CCATGAGCCA TATTACAAGA TGCAGACCAG AGCTGGCAGC 2221 AGGGAGTCTG AGCAAGGGGT GTACACCTGC ACAGCACAGG GCATTTGGAA GAATGAACAG 2281 AAGGGAGAGA AGATTCCTCG GTGCTTGCCA GTGTGTGGGA AGCCCGTGAA CCCCGTGGAA 2341 CAGAGGCAGC GCATCATCGG AGGGCAAAAA GCCAAGATGG GCAACTTCCC CTGGCAGGTG 2401 TTCACCAACA TCCACGGGCG CGGGGGCGGG GCCCTGCTGG GCGACCGCTG GATCCTCACA 2461 GCTGCCCACA CCCTGTATCC CAAGGAACAC GAAGCGCAAA GCAACGCCTC TTTGGATGTG 2521 TTCCTGGGCC ACACAAATGT GGAAGAGCTC ATGAAGCTAG GAAATCACCC CATCCGCAGG 2581 GTCAGCGTCC ACCCGGACTA CCGTCAGGAT GAGTCCTACA ATTTTGAGGG GGACATCGCC 2641 CTGCTGGAGC TGGAAAATAG TGTCACCCTG GGTCCCAACC TCCTCCCCAT CTGCCTCCCT 2701 GACAACGATA CCTTCTACGA CCTGGGCTTG ATGGGCTATG TCAGTGGCTT CGGGGTCATG 2761 GAGGAGAAGA TTGCTCATGA CCTCAGGTTT GTCCGTCTGC CCGTAGCTAA TCCACAGGCC 2821 TGTGAGAACT GGCTCCGGGG AAAGAATAGG ATGGATGTGT TCTCTCAAAA CATGTTCTGT 2881 GCTGGACACC CATCTCTAAA GCAGGACGCC TGCCAGGGGG ATAGTGGGGG CGTTTTTGCA 2941 GTAAGGGACC CGAACACTGA TCGCTGGGTG GCCACGGGCA TCGTGTCCTG GGGCATCGGG 3001 TGCAGCAGGG GCTATGGCTT CTACACCAAA GTGCTCAACT ACGTGGACTG GATCAAGAAA 3061 GAGATGGAGG AGGAGGACCT CGAGGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 3121 GACATCGACT ACAAGGATGA CGATGACAAG TAGTGAGGGC CCGTTTAAAC CCGCTGATCA 3181 GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC 3241 TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG 3301 CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG 3361 GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG 3421 GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA 3481 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 3541 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 3601 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 3661 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 3721 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 3781 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 3841 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG 3901 TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 3961 TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 4021 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA 4081 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT 4141 TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG 4201 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG 4261 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG 4321 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 4381 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 4441 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT 4501 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 4561 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 4621 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 4681 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 4741 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 4801 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 4861 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 4921 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 4981 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC 5041 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT 5101 GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC 5161 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT 5221 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC 5281 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC 5341 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC 5401 GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 5461 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 5521 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 5581 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 5641 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 5701 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 5761 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 5821 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 5881 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 5941 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 6001 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 6061 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 6121 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 6181 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 6241 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 6301 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 6361 CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 6421 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 6481 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 6541 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 6601 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 6661 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 6721 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 6781 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 6841 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 6901 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 6961 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 7021 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 7081 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 7141 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 7201 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 7261 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 7321 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 7381 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 7441 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 7501 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 7561 ATTTCCCCGA AAAGTGCCAC CTGACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.