pCMV-Trpv5-3×FLAG-mCMV-EGFP pl

pCMV-Trpv5-3×FLAG-mCMV-EGFP plasmid

PVT94100

pCMV-Trpv5-3×FLAG-mCMV-EGFP plasmid Information

Plasmid name: pCMV-Trpv5(rat)-3×FLAG-mCMV-EGFP
Alias: NM_053787.3;CaT2; Ecac1;
Gene lenght: 2169bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Trpv5-3×FLAG-mCMV-EGFP plasmid Description

pCMV-Trpv5-3×FLAG-mCMV-EGFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-Trpv5-3×FLAG-mCMV-EGFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCGC CACCatgggg gtgaaaaaac cttggatcca 661 acttcagaaa cgtctcaact ggtgggtcag agaacaagac tggaaccagc atgtggatca 721 gcttcatatg ctacagcaga aaagtatttg ggagtcacca ctgcttagag cagccaagga 781 aaatgatatg tgcacactta agagacttca acatgaccag aactgtgact tccgacaaag 841 aggagctctg ggggagacag cactgcatgt agcagccctc tatgacaacc tggatgctgc 901 tataatgctg atggagactg ctccatactt ggtcacggag tctacactgt gtgagccatt 961 tgtaggtcag actgcactgc acatcgcaat tatgaaccag aatgtgaacc tggtccgtgc 1021 cctgctagcc cgaggagcca gtgcctccgc cagggctaca ggttctgcct tccatcgaag 1081 ttcccacaac ctcatctact atggagaaca tcctttgtcc tttgctgcct gtgtgggtag 1141 tgaggagatt gttagactgc tcattgagca tggagctgac attcgggccc aggactcctt 1201 gggaaataca gtactacaca tacttgtcct acagcccaac aaaacctttg cctgccagat 1261 gtacaacctg ctactctccc atgatggggg agaccacctg aagtccctgg aacttgtgcc 1321 caacaaccaa ggactcaccc ccttcaagct ggctggggtg gaaggcaaca ctgtgatgtt 1381 tcaacacctg atgcaaaaac ggaagcacat ccagtggtca ttaggacccc taacatcttc 1441 catttatgat ctcacagaaa ttgactcctg gggtgaggac ttgtcctttc tggagcttgt 1501 ggtttcctcc aagaagaaag aggctcgtca gattctagaa caaacccctg tgaaggagct 1561 ggtgagcctg aagtggaaga aatatggaca accctatttc tgcctcctgg gtatgctgta 1621 catcttctac atgatctgct tcaccacatg ctgtgtttac cgccccctca agttccgtga 1681 tgccaaccgt acacatgtta gagataacac cgtcctggaa cagaaaccac tacaggaagc 1741 gtatgtgact taccaggata aggtccggct ggtgggagaa ctcgtgacag tcattggggc 1801 tgtggttatt ctactcatag agatcccaga catcttcaga gttggtgcct ctcgctactt 1861 tggacacaca gttcttgggg gtccattcca tgtcattatc atcacatatg cctcccttgt 1921 gctactgatt atggtgatga ggcttaccag tatgaatggg gaggtggtcc ccatatccat 1981 ggccttggta ctgggctggt gtagtgtcat gtacttctcc cgaggattcc agatgctggg 2041 ccccttcaca atcatgattc agaagatgat ttttggagac ctactgcgct tctgctggct 2101 aatggctatg gtcatcctcg gatttgcctc tgccttctat atcatcttcc agacagagga 2161 tccagagagt ctgggagaat tctccgacta ccctacagca atgttcagca cctttgagct 2221 cttccttacc atcattgacg gtccggccaa ttacagtgtg gatctaccct tcatgtacca 2281 cctaacctat tttgcttttg ccatcatcgc cacactactc atgctcaatt tgttcattgc 2341 catgatgggt gacactcact ggcgagtggc ccaggagagg gacgaactct ggagagcaca 2401 ggttgtagcc accacagtga tgctggagag gaaaatgccc cgtttcctgt ggcctcgctc 2461 tggtatctgt ggttgcgaat atggcctggg agaccgctgg ttcttacggg ttgaacacca 2521 ccaggagcag aatccgtatc gagtacttcg ctatgtggaa gctttcaaat cttcagacaa 2581 ggaggaagta caagaacaac tatctgagaa gcagccttct gggactgaga ctgggacttt 2641 agctagaggc tctgtggttc tgcaaacgcc tcctttgtcc aggactacat ccctaagcag 2701 caatagccac cgtggatggg aaattctccg tcgaaacacc ctgggacatt tgaatcttgg 2761 acaagacctt ggtgagggag acggagagga gatctaccat ttcAAGCTTA CGCGTGACTA 2821 CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA 2881 GTGAGGATCC GGGCCCGTTT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA 2941 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC 3001 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT 3061 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA 3121 TGCTGGGGAT GCGGTGGGCT CTATGGTCTA GAGATATACT GAGTCATTAG GGACTTTCCA 3181 ATGGGTTTTG CCCAGTACAT AAGGTCAATA GGGGTGAATC AACAGGAAAG TCCCATTGGA 3241 GCCAAGTACA CTGAGTCAAT AGGGACTTTC CATTGGGTTT TGCCCAGTAC AAAAGGTCAA 3301 TAGGGGGTGA GTCAATGGGT TTTTCCCATT ATTGGCACGT ACATAAGGTC AATAGGGGTG 3361 AGTCATTGGG TTTTTCCAGC CAaTTTAATT AAAACGCCAT GTACTTTCCC ACCATTGACG 3421 TCAATGGGCT ATTGAAACTA ATGCAACGTG ACCTTTAAAC GGTACTTTCC CATAGCTGAT 3481 TAATGGGAAA GTACCGTTCT CGAGCCAATA CACGTCAATG GGAAGTGAAA GGGCAGCCAA 3541 AACGTAACAC CGCCCCGGTT TTCCCCTGGA AATTCCATAT TGGCACGCAT TCTATTGGCT 3601 GAGCTGCGTT CTACGTGGGT ATAAGAGGCG CGACCAGCGT CGGTACCGTC GCAGTCTTCG 3661 GTCTGACCAC CGTAGAACGC AGATCaccgg tcgccaccat ggtgagcaag ggcgaggagc 3721 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 3781 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 3841 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 3901 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 3961 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 4021 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 4081 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 4141 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 4201 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 4261 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 4321 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 4381 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat 4441 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc 4501 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta 4561 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 4621 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg 4681 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat 4741 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg 4801 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc 4861 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt 4921 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag 4981 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg 5041 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc 5101 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 5161 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 5221 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg 5281 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 5341 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 5401 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 5461 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 5521 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 5581 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat 5641 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 5701 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 5761 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 5821 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 5881 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 5941 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 6001 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 6061 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 6121 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca 6181 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 6241 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 6301 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 6361 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 6421 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac 6481 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 6541 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 6601 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg 6661 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt 6721 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca 6781 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga 6841 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc 6901 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 6961 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 7021 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 7081 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 7141 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 7201 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 7261 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 7321 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 7381 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 7441 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 7501 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 7561 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 7621 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 7681 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 7741 taccgccatg cat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.