pLV3-CMV-Mmp13-3×FLAG-CopGFP-P

pLV3-CMV-Mmp13-3×FLAG-CopGFP-Puro plasmid

PVT96622

pLV3-CMV-Mmp13-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Mmp13(rat)-3×FLAG-CopGFP-Puro
Alias: NM_133530.1
Gene lenght: 1416bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Mmp13-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Mmp13-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Mmp13-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc attcagctat tctggccacc 2341 ttcttcttgt tgagttggac tcactgttgg tccctgcccc ttccctatgg tgatgatgat 2401 gatgatgact tgtctgagga agatcttgag tttgcagagc actacttgaa atcatactac 2461 catcctgtga ctcttgcggg aatcctgaag aagtctacag tgacctctac agttgataga 2521 ctccgagaaa tgcagtcttt ctttggctta gatgtgactg gcaaacttga tgatcctacc 2581 ttagacatca tgagaaaacc aagatgtgga gtgcctgatg tgggtgaata caatgttttc 2641 cctcgaacac tcaaatggtc ccaaacaaac ttaacttaca gaattgtgaa ctacacccct 2701 gatatatccc attctgaagt ggagaaggcc ttcagaaaag ccttcaaggt ctggtctgat 2761 gtgacacctc tgaattttac caggatccat gatggcactg ctgatatcat gatatctttt 2821 ggaactaaag aacatggtga cttctaccca tttgatgggc cttctggtct tctggcacac 2881 gcttttcctc ctggaccaaa ccttggcggg gacgcccatt ttgatgatga tgaaacctgg 2941 acaagcagct ccaaaggcta caacttattt attgttgctg cccatgagct tggccactcc 3001 ctcggtctgg accactccaa ggaccctgga gccctgatgt ttcccatcta tacctacact 3061 ggcaaaagcc atttcatgct cccagatgat gacgttcaag gaatccagtc tctctatggt 3121 ccaggagatg aagaccccaa ccctaagcac cccaaaacac cagagaagtg tgacccagcc 3181 ctatcccttg atgccattac tagtctccga ggagaaacca tgatctttaa agacagattc 3241 ttctggcgtc tgcaccctca gcaggttgag cctgaactgt ttttgaccaa gtccttttgg 3301 ccagaacttc ccaaccatgt ggatgctgca tacgagcatc catcccgaga cctcatgttc 3361 atctttagag ggagaaaatt ctgggccctg aatgggtatg acattatgga gggttatccc 3421 agaaaaatat ctgacctggg atttccaaaa gaggtgaaaa ggctcagtgc tgcggttcac 3481 tttgaggaca cggggaagac cctcttcttc tcagggaacc acgtgtggag ttatgatgat 3541 gctaaccaga ctatggacaa agactatccc cgcctcatag aagaagaatt ccctggaatt 3601 ggcgacaaag tagatgctgt ctatgagaaa aatggctata tctacttttt caatgggccc 3661 atacagtttg aatacagtat ctggagtaat cgcattgtga gagtcatgcc aacaaattcc 3721 ttattgtggt gtggatccGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC 3781 GACTACAAGG ATGACGATGA CAAGtagcgg ccgcGaagga tctgcgatcg ctccggtgcc 3841 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 3901 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 3961 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 4021 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 4081 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 4141 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 4201 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 4261 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 4321 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag 4381 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac 4441 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc 4501 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg 4561 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg 4621 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc 4681 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag 4741 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc 4801 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc 4861 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac 4921 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc 4981 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc 5041 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc 5101 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt 5161 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc 5221 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 5281 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 5341 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 5401 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 5461 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 5521 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 5581 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 5641 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 5701 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 5761 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg 5821 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 5881 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 5941 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 6001 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 6061 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 6121 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaA 6181 TCATCGTCCT TTCCTtggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 6241 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 6301 gctctgcggc ctcttccgcg tctTcgcctt cgccctcaga cgagtcggat ctccctttgg 6361 Gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 6421 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaGataaga 6481 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 6541 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 6601 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 6661 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 6721 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta 6781 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 6841 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc taTCCCGCCC 6901 CTAACTCCGC CCAtcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 6961 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 7021 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat 7081 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 7141 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 7201 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 7261 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 7321 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 7381 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 7441 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 7501 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 7561 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 7621 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 7681 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 7741 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 7801 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 7861 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 7921 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 7981 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 8041 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 8101 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 8161 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 8221 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 8281 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 8341 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 8401 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 8461 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 8521 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 8581 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 8641 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 8701 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 8761 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 8821 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 8881 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 8941 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 9001 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 9061 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 9121 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 9181 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 9241 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 9301 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 9361 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 9421 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 9481 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 9541 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 9601 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 9661 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.