pCMV-IQGAP3-3×HA-Puro plasmid

pCMV-IQGAP3-3×HA-Puro plasmid

PVT93440

pCMV-IQGAP3-3×HA-Puro plasmid Information

Plasmid name: pCMV-IQGAP3(human)-3×HA-Puro
Alias: NM_178229.5
Gene lenght: 4893bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-IQGAP3-3×HA-Puro plasmid Description

pCMV-IQGAP3-3×HA-Puro is a plasmid suitable for protein expression in mammalian cells.

pCMV-IQGAP3-3×HA-Puro plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCatg gagaggagag cagcgggccc aggctgggca gcctatgaac gcctcacagc 961 tgaggagatg gatgagcaga ggcggcagaa tgttgcctat cagtacctgt gccggctgga 1021 ggaggccaag cgctggatgg aggcctgcct gaaggaggag cttccttccc cggtggagct 1081 ggaggagagc cttcggaatg gagtgctgct ggccaagcta ggccactgtt ttgcaccctc 1141 cgtggttccc ttgaagaaga tctacgatgt ggagcagctg cggtaccagg caactggctt 1201 acatttccgt cacacagaca acatcaactt ttggctatct gcaatagccc acatcggtct 1261 gccttcgacc ttcttcccag agaccacgga catctatgac aaaaagaaca tgccccgggt 1321 agtctactgc atccatgctc tcagtctctt cctcttccgg ctgggattgg cccctcagat 1381 acatgatcta tacgggaaag tgaaattcac agctgaggaa ctcagcaaca tggcgtccga 1441 actggccaaa tatggcctcc agctgcctgc cttcagcaag atcgggggca tcttggccaa 1501 tgagctctcg gtggatgagg ctgcagtcca tgcagctgtt cttgccatca atgaagcagt 1561 ggagcgaggg gtggtggagg acaccctggc tgccttgcag aatcccagtg ctcttctgga 1621 gaatctccga gagcctctgg cagccgtcta ccaagagatg ctggcccagg ccaagatgga 1681 gaaggcagcc aatgccagga accatgatga cagagaaagc caggacatct atgaccacta 1741 cctaactcag gctgaaatcc agggcaatat caaccatgtc aacgtccatg gggctctaga 1801 agttgttgat gatgccctgg aaagacagag ccctgaagcc ttgctcaagg cccttcaaga 1861 ccctgccctg gccctgcgag gggtgaggag agactttgct gactggtacc tggagcagct 1921 gaactcagac agagagcaga aggcacagga gctgggcctg gtggagcttc tggaaaagga 1981 ggaagtccag gctggtgtgg ctgcagccaa cacaaagggt gatcaggaac aagccatgct 2041 ccacgctgtg cagcggatca acaaagccat ccggaggaga gtggcggctg acactgtgaa 2101 ggagctgatg tgccctgagg cccagctgcc tccagtgtac cctgttgcat cgtctatgta 2161 ccagctggag ctggcagtgc tccagcagca gcagggggag cttggccagg aggagctctt 2221 cgtggctgtg gagatgctct cagctgtggt cctgattaac cgggccctgg aggcccggga 2281 tgccagtggc ttctggagca gcctggtgaa ccctgccaca ggcctggctg aggtggaagg 2341 agaaaatgcc cagcgttact tcgatgccct gctgaaattg cgacaggagc gtgggatggg 2401 tgaggacttc ctgagctgga atgacctgca ggccaccgtg agccaggtca atgcacagac 2461 ccaggaagag actgaccggg tccttgcagt cagcctcatc aatgaggctc tggacaaagg 2521 cagccctgag aagactctgt ctgccctact gcttcctgca gctggcctag atgatgtcag 2581 cctccctgtc gcccctcggt accatctcct ccttgtggca gccaaaaggc agaaggccca 2641 ggtgacaggg gatcctggag ctgtgctgtg gcttgaggag atccgccagg gagtggtcag 2701 agccaaccag gacactaata cagctcagag aatggctctt ggtgtggctg ccatcaatca 2761 agccatcaag gagggcaagg cagcccagac tgagcgggtg ttgaggaacc ccgcagtggc 2821 ccttcgaggg gtagttcccg actgtgccaa cggctaccag cgagccctgg aaagtgccat 2881 ggcaaagaaa cagcgtccag cagacacagc tttctgggtt caacatgaca tgaaggatgg 2941 cactgcctac tacttccatc tgcagacctt ccaggggatc tgggagcaac ctcctggctg 3001 ccccctcaac acctctcacc tgacccggga ggagatccag tcagctgtca ccaaggtcac 3061 tgctgcctat gaccgccaac agctctggaa agccaacgtc ggctttgtta tccagctcca 3121 ggcccgcctc cgtggcttcc tagttcggca gaagtttgct gagcattccc actttctgag 3181 gacctggctc ccagcagtca tcaagatcca ggctcattgg cggggttata ggcagcggaa 3241 gatttacctg gagtggttgc agtattttaa agcaaacctg gatgccataa tcaagatcca 3301 ggcctgggcc cggatgtggg cagctcggag gcaatacctg aggcgtctgc actacttcca 3361 gaagaatgtt aactccattg tgaagatcca ggcatttttc cgagccagga aagcccaaga 3421 tgactacagg atattagtgc atgcacccca ccctcctctc agtgtggtac gcagatttgc 3481 ccatctcttg aatcaaagcc agcaagactt cttggctgag gcagagctgc tgaagctcca 3541 ggaagaggta gttaggaaga tccgatccaa tcagcagctg gagcaggacc tcaacatcat 3601 ggacatcaag attggcctgc tggtgaagaa ccggatcact ctgcaggaag tggtctccca 3661 ctgcaagaag ctgaccaaga ggaataagga acagctgtca gatatgatgg ttctggacaa 3721 gcagaagggt ttaaagtcgc tgagcaaaga gaaacggcag aaactagaag cataccaaca 3781 cctcttctac ctgctccaga ctcagcccat ctacctggcc aagctgatct ttcagatgcc 3841 acagaacaaa accaccaagt tcatggaggc agtgattttc agcctgtaca actatgcctc 3901 cagccgccga gaggcctatc tcctgctcca gctgttcaag acagcactcc aggaggaaat 3961 caagtcaaag gtggagcagc cccaggacgt ggtgacaggc aacccaacag tggtgaggct 4021 ggtggtgaga ttctaccgta atgggcgggg acagagtgcc ctgcaggaga ttctgggcaa 4081 ggttatccag gatgtgctag aagacaaagt gctcagcgtc cacacagacc ctgtccacct 4141 ctataagaac tggatcaacc agactgaggc ccagacaggg cagcgcagcc atctcccata 4201 tgatgtcacc ccggagcagg ccttgagcca ccccgaggtc cagagacgac tggacatcgc 4261 cctacgcaac ctcctcgcca tgactgataa gttcctttta gccatcacct catctgtgga 4321 ccaaattccg tatgggatgc gatatgtggc caaagtcctg aaggcaactc tggcagagaa 4381 attccctgac gccacagaca gcgaggtcta taaggtggtc gggaacctcc tgtactaccg 4441 cttcctgaac ccagctgtgg tggctcctga cgccttcgac attgtggcca tggcagctgg 4501 tggagccctg gctgcccccc agcgccatgc cctgggggct gtggctcagc tcctacagca 4561 cgctgcggct ggcaaggcct tctctgggca gagccagcac ctacgggtcc tgaatgacta 4621 tctggaggaa acacacctca agttcaggaa gttcatccat agagcctgcc aggtgccaga 4681 gccagaggag cgttttgcag tggacgagta ctcagacatg gtggctgtgg ccaaacccat 4741 ggtgtacatc accgtggggg agctggtcaa cacgcacagg ctgttgctgg agcaccagga 4801 ctgcattgcc cctgatcacc aagaccccct gcatgagctc ctggaggatc ttggggagct 4861 gcccaccatc cctgacctta ttggtgagag catcgctgca gatgggcaca cggacctgag 4921 caagctagaa gtgtccctga cgctgaccaa caagtttgaa ggactagagg cagatgctga 4981 tgactccaac acccgtagcc tgcttctgag caccaagcag ctgttggccg atatcataca 5041 gttccatcct ggggacaccc tcaaggagat cctgtccctc tcggcttcca gagagcaaga 5101 agcagcccac aagcagctga tgagccgacg ccaggcctgt acagcccaga caccggagcc 5161 actgcgacga caccgctcac tgacagctca ctccctcctg ccactggcag agaagcagcg 5221 gcgcgtcctg cggaacctac gccgacttga agccctgggg ttggtcagcg ccagaaatgg 5281 ctaccagggg ctagtggacg agctggccaa ggacatccgc aaccagcaca gacacaggca 5341 caggcggaag gcagagctgg tgaagctgca ggccacatta cagggcctga gcactaagac 5401 caccttctat gaggagcagg gtgactacta cagccagtac atccgggcct gcctggacca 5461 cctggccccc gactccaaga gttctgggaa ggggaagaag cagccttctc ttcattacac 5521 tgctgctcag ctcctggaaa agggtgtctt ggtggaaatt gaagatcttc ccgcctctca 5581 cttcagaaac gtcatctttg acatcacgcc gggagatgag gcaggaaagt ttgaagtaaa 5641 tgccaagttc ctgggtgtgg acatggagcg atttcagctt cactatcagg atctcctgca 5701 gctccagtat gagggtgtgg ctgtcatgaa actcttcaac aaggccaaag tcaatgtcaa 5761 ccttctcatc ttcctcctca acaagaagtt tttgcggaag CTCGAGTACC CATACGACGT 5821 ACCTGATTAT GCAACTTATC CTTATGATGT ACCTGATTAT GCTAGCTATC CTTATGATGT 5881 CCCAGACTAC GCAtagTCTA GAGGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC 5941 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG 6001 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG 6061 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA 6121 CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG 6181 CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT 6241 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC 6301 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG 6361 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 6421 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT 6481 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT 6541 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA 6601 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG 6661 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 6721 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 6781 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 6841 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 6901 GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 6961 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 7021 ACAGGATGAG GATCGTTTCG Catgaccgag tacaagccca cggtgcgcct cgccacccgc 7081 gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 7141 cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 7201 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 7261 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 7321 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 7381 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 7441 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 7501 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 7561 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 7621 acccgcaagc ccggtgcctg aGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 7681 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 7741 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 7801 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 7861 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 7921 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 7981 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 8041 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 8101 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 8161 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 8221 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 8281 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 8341 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 8401 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 8461 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 8521 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 8581 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 8641 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 8701 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 8761 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 8821 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 8881 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 8941 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 9001 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 9061 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 9121 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 9181 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 9241 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 9301 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 9361 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 9421 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 9481 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 9541 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 9601 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 9661 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 9721 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 9781 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 9841 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 9901 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 9961 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 10021 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 10081 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG 10141 ACGGATCGGG AGATCTCCCG ATCCCCTATG GT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.