pGEX-4T-3-SLC25A5 plasmid

pGEX-4T-3-SLC25A5 plasmid

PVT96583

pGEX-4T-3-SLC25A5 plasmid Information

Plasmid name: pGEX-4T-3-SLC25A5(human-opt-human)
Alias: NM_001152.5;2F1; AAC2; ANT2; T2; T3
Gene lenght: 894bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: High
Induction:

pGEX-4T-3-SLC25A5 plasmid Description

pGEX-4T-3-SLC25A5 is a plasmid suitable for protein expression in Escherichia coli.

pGEX-4T-3-SLC25A5 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccATGAC CGACGCCGCT GTTTCTTTTG 961 CCAAAGATTT CCTTGCAGGA GGTGTAGCCG CTGCAATTTC TAAGACAGCC GTCGCCCCCA 1021 TTGAAAGAGT GAAACTTCTG CTCCAGGTCC AGCATGCCAG CAAGCAAATC ACCGCCGATA 1081 AGCAGTACAA GGGGATAATC GATTGCGTAG TGCGCATCCC AAAGGAACAG GGAGTACTCA 1141 GTTTCTGGCG AGGAAATCTC GCGAATGTGA TCCGCTACTT CCCGACCCAG GCCCTGAACT 1201 TCGCTTTCAA AGATAAGTAT AAGCAGATTT TCCTCGGAGG CGTCGATAAG AGAACCCAGT 1261 TCTGGCTGTA TTTTGCAGGG AATCTGGCAT CTGGTGGAGC CGCTGGAGCC ACAAGCCTTT 1321 GCTTCGTCTA TCCCTTGGAT TTCGCCAGAA CTCGCCTCGC AGCCGATGTT GGAAAAGCTG 1381 GCGCCGAGCG CGAATTCCGG GGACTGGGAG ATTGCCTGGT TAAAATCTAC AAGAGTGATG 1441 GCATCAAGGG ACTTTATCAG GGCTTCAACG TGTCCGTGCA AGGCATAATA ATCTACAGGG 1501 CCGCCTATTT TGGAATCTAC GACACCGCTA AGGGTATGCT TCCAGACCCT AAGAACACAC 1561 ATATCGTCAT CTCATGGATG ATCGCCCAGA CCGTGACCGC AGTGGCGGGA CTGACTTCTT 1621 ACCCATTCGA CACCGTTAGG AGAAGGATGA TGATGCAGTC TGGGAGGAAG GGTACTGACA 1681 TCATGTACAC AGGGACGCTG GATTGCTGGA GGAAAATTGC CCGAGATGAG GGAGGTAAGG 1741 CTTTCTTCAA AGGGGCATGG TCCAACGTTC TGAGAGGCAT GGGAGGGGCG TTTGTATTGG 1801 TTCTCTATGA CGAGATCAAA AAGTACACCt agaattcccg ggtcgactcg agcggccgca 1861 tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca 1921 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 1981 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg 2041 tagcgatagc ggagtgtata attcttgaag acgaaagggc ctcgtgatac gcctattttt 2101 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa 2161 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 2221 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 2281 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 2341 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 2401 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 2461 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc 2521 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 2581 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 2641 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 2701 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 2761 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat 2821 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 2881 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 2941 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 3001 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 3061 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 3121 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 3181 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 3241 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 3301 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 3361 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 3421 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 3481 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 3541 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 3601 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 3661 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 3721 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 3781 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 3841 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 3901 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 3961 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 4021 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat 4081 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataaatt ccgacaccat 4141 cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag 4201 ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 4261 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 4321 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 4381 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 4441 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 4501 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 4561 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 4621 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 4681 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 4741 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 4801 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 4861 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 4921 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 4981 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 5041 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 5101 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 5161 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 5221 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 5281 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 5341 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 5401 acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt 5461 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc 5521 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg 5581 aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg 5641 gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt 5701 tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc gccgtttgtt 5761 cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta 5821 caggaaggcc agacgcgaat tatttttgat ggcgttggaa tt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.