pMD2.G-SV40-Tax-3×FLAG plasmid

pMD2.G-SV40-Tax-3×FLAG plasmid

PVT91738

pMD2.G-SV40-Tax-3×FLAG plasmid Information

Plasmid name: pMD2.G-SV40-Tax-3×FLAG
Alias: -
Gene lenght: 1062bpbp
Host: Mammalian cells,lentivirus
Use(s): Packaging assistant
Fragment type: ORF
Species: Human T-cell leukemia virus
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: SV40
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: AmpNR
Copy number: High
Induction:

pMD2.G-SV40-Tax-3×FLAG plasmid Description

pMD2.G-SV40-Tax-3×FLAG is a plasmid suitable for packaging assistance in mammalian cells, and can also be used for lentivirus packaging.

pMD2.G-SV40-Tax-3×FLAG plasmid Sequence

1 tggaggcaag gcctgcaaaa tgcaatactg caagcattgg ggagtcagac tcccatcagg 61 tgtctggttc gagatggctg ataaggatct ctttgctgca gccagattcc ctgaatgccc 121 agaagggtca agtatctctg ctccatctca gacctcagtg gatgtaagtc taattcagga 181 cgttgagagg atcttggatt attccctctg ccaagaaacc tggagcaaaa tcagagcggg 241 tcttccaatc tctccagtgg atctcagcta tcttgctcct aaaaacccag gaaccggtcc 301 tgctttcacc ataatcaatg gtaccctaaa atactttgag accagataca tcagagtcga 361 tattgctgct ccaatcctct caagaatggt cggaatgatc agtggaacta ccacagaaag 421 ggaactgtgg gatgactggg caccatatga agacgtggaa attggaccca atggagttct 481 gaggaccagt tcaggatata agtttccttt atacatgatt ggacatggta tgttggactc 541 cgatcttcat cttagctcaa aggctcaggt gttcgaacat cctcacattc aagacgctgc 601 ttcgcaactt cctgatgatg agagtttatt ttttggtgat actgggctat ccaaaaatcc 661 aatcgagctt gtagaaggtt ggttcagtag ttggaaaagc tctattgcct cttttttctt 721 tatcataggg ttaatcattg gactattctt ggttctccga gttggtatcc atctttgcat 781 taaattaaag cacaccaaga aaagacagat ttatacagac atagagatga accgacttgg 841 aaagtaactc aaatcctgca caacagattc ttcatgtttg gaccaaatca acttgtgata 901 ccatgctcaa agaggcctca attatatttg agtttttaat ttttatgaaa aaaaaaaaaa 961 aaaacggaat tcaccccacc agtgcaggct gcctatcaga aagtggtggc tggtgtggct 1021 aatgccctgg cccacaagta tcactaagct cgctttcttg ctgtccaatt tctattaaag 1081 gttcctttgt tccctaagtc caactactaa actgggggat attatgaagg gccttgagca 1141 tctggattct gcctaataaa aaacatttat tttcattgca atgatgtatt taaattattt 1201 ctgaatattt tactaaaaag ggaatgtggg aggtcagtgc atttaaaaca taaagaaatg 1261 aagagctagt tcaaaccttg ggaaaataca ctatatctta aactccatga aagaaggtga 1321 ggctgcaaac agctaatgca cattggcaac agcccctgat gcctatgcct tattcatccc 1381 tcagaaaagg attcaagtag aggcttgatt tggaggttaa agttttgcta tgctgtattt 1441 tacattactt attgttttag ctgtcctcat gaatgtcttt tcactaccca tttgcttatc 1501 ctgcatctct cagccttgac tccactcagt tctcttgctt agagatacca cctttcccct 1561 gaagtgttcc ttccatgttt tacggcgaga tggtttctcc tcgcctggcc actcagcctt 1621 agttgtctct gttgtcttat agaggtctac ttgaagaagg aaaaacaggg ggcatggttt 1681 gactgtcctg tgagcccttc ttccctgcct cccccactca cagtgacccg gaatccctcg 1741 acatggcagt ctagcactag tgcggccgca gatctgcttc ctcgctcact gactcgctgc 1801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 1861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 1921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 1981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 2041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 2101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 2161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 2221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 2281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 2341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 2401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 2461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 2521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 2581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 2641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 2701 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 2761 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 2821 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 2881 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 2941 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 3001 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 3061 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 3121 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 3181 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 3241 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 3301 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 3361 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 3421 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 3481 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 3541 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 3601 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 3661 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtggat cccctgaggg 3721 ggcccGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 3781 AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG 3841 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC 3901 CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA 3961 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT 4021 GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAggatc ccgtggagac tcctcaagcg 4081 agctgGCCAC CATGcccaag acccgtcgga ggccccgccg atcccaaaga aaaagaccac 4141 caacaTccat ggcccacttc ccagggtttg gacagagtct tcttttcgga tacccagtct 4201 acgtgtttgg agactgtgta caaggcgact ggtgccccat ctctggggga ctatgttcgg 4261 cccgcctaca tcgtcacgcc ctactggcca cctgtccaga gcatcagatc acctgggacc 4321 ccatcgatgg acgcgttatc ggctcagctc tacagttcct tatccctcga ctcccctcct 4381 tccccaccca gagaacctct aagaccctca aggtccttac cccgccaatc actcatacaa 4441 cccccaacat tccaccctcc ttcctccagg ccatgcgcaa atactccccc ttccgaaatg 4501 gatacatgga acccaccctt gggcagcacc tcccaaccct gtcttttcca gaccccggac 4561 tccggcccca aaacctgtac accctctggg gaggctccgt tgtctgcatg tacctctacc 4621 agctttcccc ccccatcacc tggcccctcc tgccccacgt gattttttgc caccccggcc 4681 agctcggggc cttcctcacc aatgttccct acaagcgaat agaagaactc ctctataaaa 4741 tttcccttac cacaggggcc ctaatgattc tccccgaaga ctgtttgccc accacccttt 4801 tccagcctgt tagggcaccc gtcacgctaa cagcctggca aaacggcctc cttccgttcc 4861 actcaaccct caccactcca ggccttattt ggacatttac cgatggcacg cctatgattt 4921 ccgggccctg ccctaaagat ggccagccat ctttagtact acagtcctcc tcctttatat 4981 ttcacaaatt tcaaaccaag gcctaccacc cctcatttct actctcacac ggccttatac 5041 agtactcttc ctttcataat ttacatctcc tgtttgaaga atacaccaac atccccattt 5101 ctctactttt taacgaaaaa gaggcagatg acaatgacca tgagccccaa atatcccccg 5161 ggggcttaga gccgcccagt gaaaaacatt tccgcgaaac agaagtcGAC TACAAAGACC 5221 ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGtgta 5281 cattatattg gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat 5341 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 5401 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 5461 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 5521 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 5581 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 5641 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 5701 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 5761 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 5821 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 5881 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca 5941 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctcccctc gaagcttaca 6001 tgtggtaccg agctcggatc ctgagaactt cagggtgagt ctatgggacc cttgatgttt 6061 tctttcccct tcttttctat ggttaagttc atgtcatagg aaggggagaa gtaacagggt 6121 acacatattg accaaatcag ggtaattttg catttgtaat tttaaaaaat gctttcttct 6181 tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg 6241 gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat 6301 ttctgggtta aggcaatagc aatatttctg catataaata tttctgcata taaattgtaa 6361 ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt 6421 attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc 6481 atgttcatac ctcttatctt cctcccacag ctcctgggca acgtgctggt ctgtgtgctg 6541 gcccatcact ttggcaaagc acgtgagatc tgaattctga cactatgaag tgccttttgt 6601 acttagcctt tttattcatt ggggtgaatt gcaagttcac catagttttt ccacacaacc 6661 aaaaaggaaa ctggaaaaat gttccttcta attaccatta ttgcccgtca agctcagatt 6721 taaattggca taatgactta ataggcacag ccttacaagt caaaatgccc aagagtcaca 6781 aggctattca agcagacggt tggatgtgtc atgcttccaa atgggtcact acttgtgatt 6841 tccgctggta tggaccgaag tatataacac attccatccg atccttcact ccatctgtag 6901 aacaatgcaa ggaaagcatt gaacaaacga aacaaggaac ttggctgaat ccaggcttcc 6961 ctcctcaaag ttgtggatat gcaactgtga cggatgccga agcagtgatt gtccaggtga 7021 ctcctcacca tgtgctggtt gatgaataca caggagaatg ggttgattca cagttcatca 7081 acggaaaatg cagcaattac atatgcccca ctgtccataa ctctacaacc tggcattctg 7141 actataaggt caaagggcta tgtgattcta acctcatttc catggacatc accttcttct 7201 cagaggacgg agagctatca tccctgggaa aggagggcac agggttcaga agtaactact 7261 ttgcttatga aac

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.