pCMV-EGFP-TRIM65-3×FLAG-Neo pl

pCMV-EGFP-TRIM65-3×FLAG-Neo plasmid

PVT94812

pCMV-EGFP-TRIM65-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-EGFP-TRIM65(human)-3×FLAG-Neo
Alias: NM_173547.4;4732463G12Rik
Gene lenght: 1551bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: BGH-R
Copy number: High
Induction:

pCMV-EGFP-TRIM65-3×FLAG-Neo plasmid Description

pCMV-EGFP-TRIM65-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-TRIM65-3×FLAG-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcG 961 CCACCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC 1021 TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA 1081 CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC 1141 CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA 1201 TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA 1261 TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA 1321 CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG 1381 GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA 1441 AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC 1501 TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA 1561 ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA 1621 TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA 1681 AGgGaGGCGG AGGTAGTGGA GGAGGGGGAA GCggaggcgg agggagtGAA TTCatggccg 1741 cgcagctgct ggaggagaag ctgacctgcg ccatctgcct ggggctctac caggacccag 1801 tgacgctgcc ctgcggccac aacttctgcg gggcctgcat ccgggactgg tgggaccgct 1861 gcggaaaggc gtgccccgag tgccgggagc cctttcccga cggcgccgag ctgcgccgca 1921 acgtggccct cagcggcgtg ctggaggtgg tgcgcgccgg gcccgcccgg gatcccggcc 1981 ccgatcccgg ccccggcccc gaccctgccg cgcgctgccc ccgccacggg cggccgctgg 2041 agctcttctg ccggaccgag ggccgctgtg tgtgcagcgt gtgcaccgtg cgcgagtgtc 2101 gcctccacga gcgggcgctg ctggatgccg agcgcctcaa gcgcgaggcc cagctgagag 2161 ccagcctgga ggttacccag cagcaggcca cccaggccga aggccagcta ctagagctgc 2221 gcaagcaaag cagccagatc cagaactcgg cctgcatctt ggcctcctgg gtctccggca 2281 agttcagcag cctgctacag gccctggaaa tacagcacac gacagcactg aggagcatcg 2341 aggtggccaa gacgcaggcg ctggcacagg ctcgagacga ggagcagcgg ctgcgggtcc 2401 atttggaggc tgtggctcgc catggctgca ggatccggga gctcctggag caggtggatg 2461 agcagacctt cctgcaggaa tcgcagctcc tccagccccc agggcctctt gggccactga 2521 cccctctgca gtgggatgaa gaccaacagc tgggtgacct gaagcagttg ctaagccggc 2581 tgtgtggcct cctcttggaa gaggggagcc accctggggc accagccaag cctgtggact 2641 tagcccccgt ggaggcccca ggtcccctgg caccggtccc aagcacagtt tgtccactga 2701 ggaggaaact ctggcagaat tatcgcaatc tgacctttga tccagtcagc gccaaccgtc 2761 acttctatct gtcgcgccag gaccagcagg tgaagcactg tcgtcagtcc cggggcccag 2821 gcgggcccgg cagctttgag ctctggcagg tgcaatgtgc ccagagcttc caggccgggc 2881 accactactg ggaggtgcgc gcgtcagacc actcggtgac actgggcgtc tcctacccgc 2941 aactgccacg gtgcaggctg gggccccaca cagacaacat tggccgggga ccctgctcct 3001 gggggctctg cgtccaggag gacagcctcc aggcctggca caacggggaa gcccagcgcc 3061 tcccaggggt gtcagggcgg ctcctgggca tggatttgga cctggcctca ggctgcctca 3121 ccttctacag cctggagccc cagacccagc ccctgtacac cttccatgcc ctcttcaacc 3181 agcccctcac ccccgtcttc tggctcctcg agggtaggac cctgaccctg tgccatcagc 3241 caggggctgt gttccctctg gggccccagg aagaggtgct cagcGACTAC AAAGACCATG 3301 ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGACAAG tagcttaagt 3361 ttaaaccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc 3421 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat 3481 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg 3541 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc 3601 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc 3661 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 3721 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 3781 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 3841 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 3901 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 3961 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 4021 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 4081 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 4141 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 4201 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 4261 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 4321 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 4381 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 4441 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac 4501 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 4561 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 4621 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 4681 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 4741 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 4801 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 4861 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 4921 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 4981 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 5041 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 5101 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 5161 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 5221 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 5281 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg 5341 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 5401 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa 5461 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 5521 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 5581 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt 5641 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 5701 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 5761 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 5821 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 5881 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 5941 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 6001 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 6061 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 6121 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 6181 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 6241 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 6301 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 6361 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 6421 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 6481 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 6541 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag 6601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 6661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 6721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 6781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 6841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 6901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 6961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 7021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 7081 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 7141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 7201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 7261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 7321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 7381 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 7441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 7501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 7561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 7621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 7681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 7741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.