pLV3-CMV-Pold3-3×FLAG-CopGFP-P

pLV3-CMV-Pold3-3×FLAG-CopGFP-Puro plasmid

PVT96563

pLV3-CMV-Pold3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Pold3(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_133692.2;2410142G14Rik; P66; P68
Gene lenght: 1383bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Pold3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Pold3-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Pold3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cggaacagct gtatctagaa 2341 aacatagacg agttcgtcac ggaccagaac aagatcgtga cttacaagtg gctaagctat 2401 acactagggg ttcatgttaa ccaggccaaa cagatgctct atgaatatgt tgaaaggaaa 2461 cggaaagaaa attcgggagc tcagctgcat gttacctact tggtgtctgg cagtcttata 2521 cagaacggac attcttgcca caaggttgca gtagtgagag aagataaact ggaagcagtg 2581 aagtccaagt tagctgtgac tgccagcatc catgtgtaca gcatccagaa agctatgcta 2641 aaggacagtg ggcctctgtt caataccgac tatgacatcc ttaaaagcaa tttgcagaac 2701 tgcagcaagt ttagtgccat acagtgtgca gctgcagtcc ccagagctcc tgcagaatcc 2761 ccatcttcca gaaagtatga acagtcaaat cttcaggcag cgagtgaggc acaagccagt 2821 gagctgacta ccaatggcca tggtccacct gcctccaaac aggcttccca gcagcccaaa 2881 ggaattatgg gaatgttaat ctctaaagct gctactaaaa cccaggacac caacaaggaa 2941 accaaaccag aggcccgaga agtaaccagt gcatcttctg ctgggggcaa agcaccagga 3001 aaagggagtg tgatgagcaa cttttttgga aaagctgcaa tgaataaact taaagtcaat 3061 ttggattcag aacaagcagt aaaggaagaa aaaacagtgg agcaacctcc agtgtctgtc 3121 actgaaccaa agctggcagc tcccccagct cagaagaaat ccagcagaaa gtccgagcct 3181 gggaaggtgc agcagaagga gaaaagcagg ggcaagcgag tagacttgtc ggatgaggag 3241 gcaaaggaaa ccgaacacct gaagaaaaag agaagaagaa tcaagcttcc tcagtctgat 3301 agcagtgaag atgaagtctt cgaagactcc cctgagatgt atgaagcaga ctcaccatct 3361 ccacctcctg tatctccacc tcctgatcct atgccaaaaa ctgagccccc tcctgtcaag 3421 cgttcaagtg gagaaaccaa aaggagacga aagcgtgtac tgaaatctaa aacctttgtg 3481 gatgaagaag gctgcatagt gactgagaaa gtctatgaga gtgagtcctg cacagacagt 3541 gaggaggagc ttaagatgaa gccggcctca gcacacaaac cccctgctgc cgctgtgaaa 3601 agggagccca gagaagaacg gaagggcccc aagaaagggg ctgctgctct gggcaaagcc 3661 aacagacaag tgtccattac tggcttcttc cagaaaaagg gatccGACTA CAAAGACCAT 3721 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg 3781 cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 3841 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 3901 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 3961 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 4021 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 4081 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 4141 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 4201 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 4261 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 4321 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc 4381 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag 4441 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc 4501 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac 4561 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc 4621 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag 4681 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg 4741 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc 4801 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac 4861 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag 4921 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg 4981 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct 5041 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag 5101 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag 5161 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 5221 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 5281 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 5341 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 5401 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 5461 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 5521 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 5581 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 5641 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 5701 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct 5761 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 5821 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 5881 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 5941 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 6001 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 6061 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 6121 gacaattccg tggtgttgtc ggggaaATCA TCGTCCTTTC CTtggctgct cgcctgtgtt 6181 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 6241 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct Tcgccttcgc 6301 cctcagacga gtcggatctc cctttggGcc gcctccccgc ctggtacctt taagaccaat 6361 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 6421 gctaattcac tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt 6481 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 6541 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 6601 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 6661 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 6721 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 6781 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 6841 tcatgtctgg ctctagctaT CCCGCCCCTA ACTCCGCCCA tcccgcccct aactccgccc 6901 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 6961 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 7021 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc 7081 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 7141 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 7201 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 7261 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 7321 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 7381 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 7441 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 7501 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 7561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 7621 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 7681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 7741 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 7801 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 7861 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 7921 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7981 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 8041 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 8101 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 8161 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 8221 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 8281 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 8341 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 8401 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 8461 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 8521 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 8581 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 8641 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 8701 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 8761 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 8821 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 8881 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 8941 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 9001 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 9061 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 9121 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 9181 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 9241 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 9301 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 9361 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 9421 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 9481 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 9541 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 9601 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 9661 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.