pLV3-CMV-ACADVL-3×FLAG-CopGFP-

pLV3-CMV-ACADVL-3×FLAG-CopGFP-Puro plasmid

PVT93972

pLV3-CMV-ACADVL-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ACADVL(human)-3×FLAG-CopGFP-Puro
Alias: NM_000018.4;ACAD6; LCACD; VLCAD
Gene lenght: 1965bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ACADVL-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-ACADVL-3×FLAG-CopGFP-Puro is a plasmid suitable for protein expression in mammalian cells.

pLV3-CMV-ACADVL-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgc aggcggctcg gatggccgcg 2341 agcttggggc ggcagctgct gaggctcggg ggcggaagct cgcggctcac ggcgctcctg 2401 gggcagcccc ggcccggccc tgcccggcgg ccctatgccg ggggtgccgc tcagctggct 2461 ctggacaagt cagattccca cccctctgac gctctgacca ggaaaaaacc ggccaaggcg 2521 gaatctaagt cctttgctgt gggaatgttc aaaggccagc tcaccacaga tcaggtgttc 2581 ccatacccgt ccgtgctcaa cgaagagcag acacagtttc ttaaagagct ggtggagcct 2641 gtgtcccgtt tcttcgagga agtgaacgat cccgccaaga atgacgctct ggagatggtg 2701 gaggagacca cttggcaggg cctcaaggag ctgggggcct ttggtctgca agtgcccagt 2761 gagctgggtg gtgtgggcct ttgcaacacc cagtacgccc gtttggtgga gatcgtgggc 2821 atgcatgacc ttggcgtggg cattaccctg ggggcccatc agagcatcgg tttcaaaggc 2881 atcctgctct ttggcacaaa ggcccagaaa gaaaaatacc tccccaagct ggcatctggg 2941 gagactgtgg ccgctttctg tctaaccgag ccctcaagcg ggtcagatgc agcctccatc 3001 cgaacctctg ctgtgcccag cccctgtgga aaatactata ccctcaatgg aagcaagctt 3061 tggatcagta atgggggcct agcagacatc ttcacggtct ttgccaagac accagttaca 3121 gatccagcca caggagccgt gaaggagaag atcacagctt ttgtggtgga gaggggcttc 3181 gggggcatta cccatgggcc ccctgagaag aagatgggca tcaaggcttc aaacacagca 3241 gaggtgttct ttgatggagt acgggtgcca tcggagaacg tgctgggtga ggttgggagt 3301 ggcttcaagg ttgccatgca catcctcaac aatggaaggt ttggcatggc tgcggccctg 3361 gcaggtacca tgagaggcat cattgctaag gcggtagatc atgccactaa tcgtacccag 3421 tttggggaga aaattcacaa ctttgggctg atccaggaga agctggcacg gatggttatg 3481 ctgcagtatg taactgagtc catggcttac atggtgagtg ctaacatgga ccagggagcc 3541 acggacttcc agatagaggc cgccatcagc aaaatctttg gctcggaggc agcctggaag 3601 gtgacagatg aatgcatcca aatcatgggg ggtatgggct tcatgaagga acctggagta 3661 gagcgtgtgc tccgagatct tcgcatcttc cggatctttg aggggacaaa tgacattctt 3721 cggctgtttg tggctctgca gggctgtatg gacaaaggaa aggagctctc tgggcttggc 3781 agtgctctaa agaatccctt tgggaatgct ggcctcctgc taggagaggc aggcaaacag 3841 ctgaggcggc gggcagggct gggcagcggc ctgagtctca gcggacttgt ccacccggag 3901 ttgagtcgga gtggcgagct ggcagtacgg gctctggagc agtttgccac tgtggtggag 3961 gccaagctga taaaacacaa gaaggggatt gtcaatgaac agtttctgct gcagcggctg 4021 gcagacgggg ccatcgacct ctatgccatg gtggtggttc tctcgagggc ctcaagatcc 4081 ctgagtgagg gccaccccac ggcccagcat gagaaaatgc tctgtgacac ctggtgtatc 4141 gaggctgcag ctcggatccg agagggcatg gccgccctgc agtctgaccc ctggcagcaa 4201 gagctctacc gcaacttcaa aagcatctcc aaggccttgg tggagcgggg tggtgtggtc 4261 accagcaacc cacttggctt cggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 4321 CATGACATCG ACTACAAGGA TGACGATGAC AAGtagcggc cgcGaaggat ctgcgatcgc 4381 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 4441 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 4501 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 4561 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 4621 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 4681 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 4741 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 4801 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 4861 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 4921 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc 4981 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc 5041 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg 5101 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac 5161 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag 5221 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc 5281 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc 5341 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc 5401 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc 5461 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc 5521 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag 5581 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc 5641 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta 5701 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc 5761 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 5821 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 5881 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 5941 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 6001 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 6061 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 6121 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 6181 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 6241 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 6301 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt 6361 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 6421 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 6481 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 6541 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 6601 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 6661 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 6721 tcggggaaAT CATCGTCCTT TCCTtggctg ctcgcctgtg ttgccacctg gattctgcgc 6781 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 6841 ctgctgccgg ctctgcggcc tcttccgcgt ctTcgccttc gccctcagac gagtcggatc 6901 tccctttggG ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 6961 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 7021 aaGataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 7081 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 7141 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 7201 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 7261 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 7321 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 7381 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 7441 aTCCCGCCCC TAACTCCGCC CAtcccgccc ctaactccgc ccagttccgc ccattctccg 7501 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 7561 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt 7621 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 7681 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 7741 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 7801 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 7861 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 7921 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 7981 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 8041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 8101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 8161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 8221 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 8281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 8341 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 8401 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 8461 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 8521 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 8581 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 8641 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 8701 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 8761 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 8821 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 8881 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 8941 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 9001 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 9061 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 9121 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 9181 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 9241 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 9301 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 9361 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 9421 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 9481 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 9541 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 9601 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 9661 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 9721 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 9781 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 9841 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 9901 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 9961 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 10021 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 10081 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 10141 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 10201 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.