pLV3-CMV-SDF2L1-3×FLAG-CopGFP-

pLV3-CMV-SDF2L1-3×FLAG-CopGFP-Puro plasmid

PVT96528

pLV3-CMV-SDF2L1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SDF2L1(human)-3×FLAG-CopGFP-Puro
Alias: NM_022044.3
Gene lenght: 663bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SDF2L1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-SDF2L1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SDF2L1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgt ggagcgcggg ccgcggcggg 2341 gctgcctggc cggtgctgtt ggggctgctg ctggcgctgt tagtgccggg cggtggtgcc 2401 gccaagaccg gtgcggagct cgtgacctgc gggtcggtgc tgaagctgct caatacgcac 2461 caccgcgtgc ggctgcactc gcacgacatc aaatacggat ccggcagcgg ccagcaatcg 2521 gtgaccggcg tagaggcgtc ggacgacgcc aatagctact ggcggatccg cggcggctcg 2581 gagggcgggt gcccgcgcgg gtccccggtg cgctgcgggc aggcggtgag gctcacgcat 2641 gtgcttacgg gcaagaacct gcacacgcac cacttcccgt cgccgctgtc caacaaccag 2701 gaggtgagtg cctttgggga agacggcgag ggcgacgacc tggacctatg gacagtgcgc 2761 tgctctggac agcactggga gcgtgaggct gctgtgcgct tccagcatgt gggcacctct 2821 gtgttcctgt cagtcacggg tgagcagtat ggaagcccca tccgtgggca gcatgaggtc 2881 cacggcatgc ccagtgccaa cacgcacaat acgtggaagg ccatggaagg catcttcatc 2941 aagcctagtg tggagccctc tgcaggtcac gatgaactcg gatccGACTA CAAAGACCAT 3001 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg 3061 cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 3121 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 3181 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 3241 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 3301 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 3361 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 3421 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 3481 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 3541 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 3601 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc 3661 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag 3721 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc 3781 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac 3841 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc 3901 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag 3961 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg 4021 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc 4081 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac 4141 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag 4201 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg 4261 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct 4321 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag 4381 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag 4441 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 4501 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 4561 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 4621 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 4681 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 4741 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 4801 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 4861 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 4921 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 4981 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct 5041 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 5101 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 5161 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 5221 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 5281 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 5341 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 5401 gacaattccg tggtgttgtc ggggaaATCA TCGTCCTTTC CTtggctgct cgcctgtgtt 5461 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 5521 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct Tcgccttcgc 5581 cctcagacga gtcggatctc cctttggGcc gcctccccgc ctggtacctt taagaccaat 5641 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 5701 gctaattcac tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt 5761 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 5821 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 5881 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 5941 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 6001 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 6061 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 6121 tcatgtctgg ctctagctaT CCCGCCCCTA ACTCCGCCCA tcccgcccct aactccgccc 6181 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 6241 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 6301 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc 6361 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 6421 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 6481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 6541 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6601 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 6661 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 6721 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 6781 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 6841 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6901 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 6961 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 7021 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 7081 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 7141 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 7201 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7261 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 7321 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 7381 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 7441 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 7501 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 7561 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 7621 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 7681 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 7741 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 7801 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 7861 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 7921 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 7981 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 8041 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 8101 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 8161 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 8221 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 8281 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 8341 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 8401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 8461 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 8521 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 8581 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 8641 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 8701 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 8761 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 8821 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 8881 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 8941 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.