pLV3-CMV-3×FLAG-Map3k20-2-CopG

pLV3-CMV-3×FLAG-Map3k20-2-CopGFP-Puro plasmid

PVT96505

pLV3-CMV-3×FLAG-Map3k20-2-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-3×FLAG-Map3k20(mouse)-2-CopGFP-Puro
Alias: NM_001164791.2;B230120H23Rik; HCCS-4; MLTK; MLTKalpha;MLTKbeta; MRK; Zak
Gene lenght: 1365bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×FLAG-Map3k20-2-CopGFP-Puro plasmid Description

pLV3-CMV-3×FLAG-Map3k20-2-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-3×FLAG-Map3k20-2-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 caccatgGAC TACAAGGATG ACGATGACAA GGATTACAAA GACGACGATG ATAAGGACTA 2341 TAAGGATGAT GACGACAAAg aattcatgtc gtctctcgga gcctcctttg tgcaaattaa 2401 gttcgacgac ttgcagtttt ttgaaaactg cggtggagga agttttggga gtgtgtatcg 2461 agccaaatgg atatcacagg acaaggaggt ggctgtaaag aagttactca aaatagagaa 2521 agaggcagaa atcctgagcg tcctcagtca cagaaacatc atccagtttt atggagtgat 2581 tctggaacct cccaactatg gcatcgtcac agaatatgct tccctgggct ccctgtatga 2641 ttacattaac agcaacagga gtgaagagat ggacatggaa cacatcatga cctgggccac 2701 tgacgtagcc aaagggatgc attacttaca catggaagct cctgtcaagg tgatccacag 2761 agacctcaag tcaagaaacg ttgttatcgc tgctgacgga gtgctgaaga tctgtgactt 2821 cggtgcctcg cggttccata accacacaac acacatgtcc ttggttggaa ctttcccatg 2881 gatggctcca gaagttatcc agagtctccc tgtgtctgaa acctgtgaca cgtattccta 2941 tggtgtggtt ctctgggaga tgctaacaag ggaggtcccc tttaaaggtt tggaaggatt 3001 acaagtagct tggcttgtag tggaaaaaaa cgagagatta accattccaa gcagctgccc 3061 cagaagcttt gctgaactgc tacaccagtg ttgggaggct gatgccaaga aacggccatc 3121 gttcaagcaa atcatttcaa tcctggagtc tatgtcaaat gacacgaacc ttcctgacca 3181 gtgtaactca tttttacaca acaaggcgga gtggaggtgt gaaattgagg caacccttga 3241 gcgactgaag aaactagagc gagatctcag ctttaaagag caggagctca aagaacggga 3301 gagacgtctc aagatgtggg agcagaagct gacggagcaa tccaacaccc cgcttctctt 3361 gcctctctct gcaagaatgt ctgaggagtc ttactttgaa tctaaaacag aggagtcaaa 3421 cagtgcagag atgtcatgcc agatcactgc agcaagtaac ggggagggcc atggcatgaa 3481 cccaggcctg caggccatga tgctcatggg ctttggggat gtcttctcaa tgaacaaagc 3541 aggagctgtg ctgcattctg ggatgcagat aaacatgcaa gccaagcaga attcatccaa 3601 aaccacatgt aagaggagag ggaagaaagt caacatggcc ctggggttca gtgactttga 3661 cctgtcagaa ggtgacgatg atgaccatga tggtgacgat gctgagaatg atgtggataa 3721 tagtgaatga ggatccgcgg ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg 3781 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg 3841 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc 3901 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt 3961 ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt 4021 cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc 4081 ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt 4141 cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca 4201 cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc 4261 gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag agcgacgaga 4321 gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt 4381 tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga 4441 agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg 4501 gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca 4561 acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg 4621 tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca 4681 ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg 4741 tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca 4801 gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg 4861 ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg 4921 agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca 4981 tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac 5041 ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt gacgtggagg 5101 agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg 5161 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca 5221 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc 5281 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct 5341 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg 5401 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc 5461 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg 5521 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg 5581 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct 5641 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca 5701 agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 5761 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 5821 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 5881 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 5941 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 6001 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 6061 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 6121 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 6181 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 6241 ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc 6301 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 6361 aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg 6421 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 6481 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 6541 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 6601 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 6661 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca 6721 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 6781 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc 6841 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 6901 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga 6961 cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg 7021 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 7081 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 7141 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 7201 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 7261 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 7321 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 7381 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7441 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7501 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7561 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7621 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7681 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7741 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7801 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 7861 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7921 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 7981 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 8041 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 8101 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 8161 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 8221 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 8281 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 8341 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 8401 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8461 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 8521 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 8581 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8641 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8701 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8761 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8821 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8881 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8941 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 9001 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 9061 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 9121 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 9181 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 9241 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 9301 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 9361 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 9421 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 9481 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 9541 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 9601 gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.