pLV3-CMV-MCU-3×HA-Hygro plasmi

pLV3-CMV-MCU-3×HA-Hygro plasmid

PVT96512

pLV3-CMV-MCU-3×HA-Hygro plasmid Information

Plasmid name: pLV3-CMV-MCU(human)-3×HA-Hygro
Alias: NM_138357.3;C10orf42; CCDC109A; HsMCU
Gene lenght: 1053bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MCU-3×HA-Hygro plasmid Description

pLV3-CMV-MCU-3×HA-Hygro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MCU-3×HA-Hygro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagccacca tggcggccgc cgcaggtaga tcgctcctgc tgctcctctc ctctcggggc 2341 ggcggcggcg ggggcgccgg cggctgcggg gcgctgactg ccggctgctt ccctgggctg 2401 ggcgtcagcc gccaccggca gcagcagcac caccggacgg tacaccagag gatcgcttcc 2461 tggcagaatt tgggagctgt ttattgcagc actgttgtgc cctctgatga tgttacagtg 2521 gtttatcaaa atgggttacc tgtgatatct gtgaggctac catcccggcg tgaacgctgt 2581 cagttcacac tcaagcctat ctctgactct gttggtgtat ttttacgaca actgcaagaa 2641 gaggatcggg gaattgacag agttgctatc tattcaccag atggtgttcg cgttgctgct 2701 tcaacaggaa tagacctcct cctccttgat gactttaagc tggtcattaa tgacttaaca 2761 taccacgtac gaccaccaaa aagagacctc ttaagtcatg aaaatgcagc aacgctgaat 2821 gatgtaaaga cattggtcca gcaactatac accacactgt gcattgagca gcaccagtta 2881 aacaaggaaa gggagcttat tgaaagacta gaggatctca aagagcagct ggctcccctg 2941 gaaaaggtac gaattgagat tagcagaaaa gctgagaaga ggaccacttt ggtgctatgg 3001 ggtggccttg cctacatggc cacacagttt ggcattttgg cccggcttac ctggtgggaa 3061 tattcctggg acatcatgga gccagtaaca tacttcatca cttatggaag tgccatggca 3121 atgtatgcat attttgtaat gacacgccag gaatatgttt atccagaagc cagagacaga 3181 caatacttac tatttttcca taaaggagcc aaaaagtcac gttttgacct agagaaatac 3241 aatcaactca aggatgcaat tgctcaggca gaaatggacc ttaagagact gagagaccca 3301 ttacaagtac atctgcctct ccgacaaatt ggtgaaaaag atCTCGAGTA CCCATACGAC 3361 GTACCTGATT ATGCAACTTA TCCTTATGAT GTACCTGATT ATGCTAGCTA TCCTTATGAT 3421 GTCCCAGACT ACGCATAGgg atccgcggcc gcaaggatct gcgatcgctc cggtgcccgt 3481 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 3541 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 3601 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 3661 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc 3721 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt 3781 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag 3841 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg 3901 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt 3961 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgt cgacatgaaa aagcctgaac 4021 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga 4081 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat 4141 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc 4201 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga 4261 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa 4321 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg 4381 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta 4441 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga 4501 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg 4561 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga 4621 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc 4681 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga 4741 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata 4801 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg 4861 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc 4921 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg 4981 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag gtcgacaatc 5041 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 5101 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 5161 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 5221 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 5281 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 5341 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 5401 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct 5461 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 5521 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 5581 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga 5641 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 5701 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc 5761 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 5821 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 5881 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 5941 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 6001 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 6061 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 6121 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 6181 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 6241 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 6301 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 6361 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 6421 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 6481 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 6541 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 6601 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 6661 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 6721 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 6781 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 6841 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 6901 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 6961 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 7021 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 7081 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 7141 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 7201 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 7261 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 7321 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 7381 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 7441 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 7501 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 7561 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 7621 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 7681 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 7741 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 7801 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 7861 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 7921 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 7981 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 8041 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 8101 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 8161 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 8221 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 8281 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 8341 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 8401 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 8461 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 8521 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 8581 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 8641 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 8701 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 8761 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 8821 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 8881 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 8941 tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.