pLV3-U6-VPS53-shRNA3-CopGFP-Pu

pLV3-U6-VPS53-shRNA3-CopGFP-Puro plasmid

PVT96810

pLV3-U6-VPS53-shRNA3-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-VPS53(human)-shRNA3-CopGFP-Puro
Alias: GAAAGGAGATTTAGATCAA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-VPS53-shRNA3-CopGFP-Puro plasmid Description

pLV3-U6-VPS53-shRNA3-CopGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-VPS53-shRNA3-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 1981 GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 2041 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 2101 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2161 TTGTGGAAAG GACTCATCgc tagcgaattc gGAAAGGAGA TTTAGATCAA ctcgagTTGA 2221 TCTAAATCTC CTTTCttttt tggatccgcg gccgcGaagg atctgcgatc gctccggtgc 2281 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg 2341 caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta 2401 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt 2461 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg 2521 catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg 2581 cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt 2641 aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag 2701 ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc 2761 tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga 2821 gagcgacgag agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa 2881 cggcgtggag ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac 2941 caacaagatg aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt 3001 gatgggctac ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct 3061 gcacgccatc aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg 3121 cgtgctgcac gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa 3181 ggtggtgggc accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag 3241 caacgccacc gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc 3301 ccgcaccttc agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca 3361 cttcaagagc gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg 3421 ccgcgtggag gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt 3481 caagaccccc atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg 3541 caccgccgga cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg 3601 tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac 3661 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc 3721 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact 3781 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc 3841 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg 3901 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct 3961 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc 4021 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga 4081 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga 4141 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg 4201 catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt 4261 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 4321 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 4381 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 4441 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 4501 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 4561 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 4621 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 4681 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 4741 ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg 4801 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 4861 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga 4921 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 4981 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 5041 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 5101 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 5161 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta 5221 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 5281 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc 5341 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 5401 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 5461 ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct 5521 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 5581 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 5641 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 5701 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 5761 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 5821 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 5881 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 5941 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 6001 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 6061 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 6121 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 6181 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 6241 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 6301 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 6361 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 6421 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 6481 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 6541 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 6601 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 6661 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 6721 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6781 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 6841 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 6901 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 6961 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 7021 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 7081 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 7141 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 7201 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 7261 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 7321 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 7381 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 7441 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 7501 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 7561 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 7621 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 7681 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt 7741 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa 7801 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg 7861 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 7921 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg 7981 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg 8041 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga 8101 cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.