pCMV-ER-sfGFP-KDEL-Neo plasmid

pCMV-ER-sfGFP-KDEL-Neo plasmid

PVT87241

pCMV-ER-sfGFP-KDEL-Neo plasmid Information

Plasmid name: pCMV-ER-sfGFP-KDEL-Neo
Alias: -
Gene lenght:
Host: Mammalian cells
Use(s): subcellular localization
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pDsRed2-ER
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-ER-sfGFP-KDEL-Neo plasmid Description

pCMV-ER-sfGFP-KDEL-Neo This plasmid is suitable for overexpression in mammalian cells. The CMV strong promoter carried by the plasmid can initiate the transcription of downstream genes. The plasmid carries the ER gene, ensuring that the protein is correctly localized to the endoplasmic reticulum and retained there to perform specific functions. The plasmid carries the sfGFP green fluorescent protein for observing the expression and localization of the target gene. The plasmid carries the Neo/G418 resistance gene for selecting host cells that express the target gene.

pCMV-ER-sfGFP-KDEL-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgc 601 tgctatccgt gccgttgctg ctcggcctcc tcggcctggc cgtcgccgac cggtcgcaca 661 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 721 acggcgacgt aaacggccac aagttcagcg tgcgcggcga gggcgagggc gatgccacca 781 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 841 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 901 agcgccacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatca 961 gcttcaagga cgacggcacc tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 1021 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 1081 acaagctgga gtacaacttc aacagccaca acgtctatat caccgccgac aagcagaaga 1141 acggcatcaa ggccaacttc aagatccgcc acaacgtgga ggacggcagc gtgcagctcg 1201 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 1261 actacctgag cacccagtcc gtgctgagca aagaccccaa cgagaagcgc gatcacatgg 1321 tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag ctgtacaaga 1381 gatcgtacaa gaaggacgag ctgtaaagat ctcgagctca agcttcgaat tctgcagtcg 1441 acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc agccatacca 1501 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac 1561 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat 1621 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 1681 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta 1741 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 1801 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg 1861 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 1921 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc 1981 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 2041 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 2101 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 2161 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 2221 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 2281 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt 2341 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 2401 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 2461 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 2521 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 2581 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 2641 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt 2701 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 2761 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 2821 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac 2881 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 2941 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 3001 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 3061 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 3121 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 3181 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag 3241 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 3301 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 3361 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 3421 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 3481 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat 3541 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 3601 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 3661 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga 3721 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg 3781 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg 3841 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg 3901 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga 3961 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 4021 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 4081 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 4141 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 4201 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 4261 agttaggcca ccacttcaag aactctgtat caccgcctac atacctcgct ctgctaatcc 4321 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 4381 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 4441 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 4501 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 4561 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 4621 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 4681 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 4741 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.