pLV3-CMV-lgk-LAMP2-anti-PDL1-n

pLV3-CMV-lgk-LAMP2-anti-PDL1-nanobody-mCherry-Puro plasmid

PVT93526

pLV3-CMV-lgk-LAMP2-anti-PDL1-nanobody-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-lgk-LAMP2(human)-anti-PDL1-nanobody-mCherry-Puro
Alias: NM_013995.2;CD107b; DND; LAMP-2; LAMPB; LGP-96; LGP110
Gene lenght: 1230bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-lgk-LAMP2-anti-PDL1-nanobody-mCherry-Puro plasmid Description

pLV3-CMV-lgk-LAMP2-anti-PDL1-nanobody-mCherry-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-lgk-LAMP2-anti-PDL1-nanobody-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggagac agacacactc ctgctatggg tactgctgct 2341 ctgggttcca ggttccactg gtgacatggt gtgcttccgc ctcttcccgg ttccgggctc 2401 agggctcgtt ctggtctgcc tagtcctggg agctgtgcgg tcttatgcat tggaacttaa 2461 tttgacagat tcagaaaatg ccacttgcct ttatgcaaaa tggcagatga atttcacagt 2521 acgctatgaa actacaaata aaacttataa aactgtaacc atttcagacc atggcactgt 2581 gacatataat ggaagcattt gtggggatga tcagaatggt cccaaaatag cagtgcagtt 2641 cggacctggc ttttcctgga ttgcgaattt taccaaggca gcatctactt attcaattga 2701 cagcgtctca ttttcctaca acactggtga taacacaaca tttcctgatg ctgaagataa 2761 aggaattctt actgttgatg aacttttggc catcagaatt ccattgaatg acctttttag 2821 atgcaatagt ttatcaactt tggaaaagaa tgatgttgtc caacactact gggatgttct 2881 tgtacaagct tttgtccaaa atggcacagt gagcacaaat gagttcctgt gtgataaaga 2941 caaaacttca acagtggcac ccaccataca caccactgtg ccatctccta ctacaacacc 3001 tactccaaag gaaaaaccag aagctggaac ctattcagtt aataatggca atgatacttg 3061 tctgctggct accatggggc tgcagctgaa catcactcag gataaggttg cttcagttat 3121 taacatcaac cccaatacaa ctcactccac aggcagctgc cgttctcaca ctgctctact 3181 tagactcaat agcagcacca ttaagtatct agactttgtc tttgctgtga aaaatgaaaa 3241 ccgattttat ctgaaggaag tgaacatcag catgtatttg gttaatggct ccgttttcag 3301 cattgcaaat aacaatctca gctactggga tgcccccctg ggaagttctt atatgtgcaa 3361 caaagagcag actgtttcag tgtctggagc atttcagata aatacctttg atctaagggt 3421 tcagcctttc aatgtgacac aaggaaagta ttctacagcc caagagtgtt cgctggatga 3481 tgacaccatt ctaatcccaa ttatagttgg tgctggtctt tcaggcttga ttatcgttat 3541 agtgattgct tacgtaattg gcagaagaaa aagttatgct ggatatcaga ctctgCAGGT 3601 TCAGCTGCAG GAATCTGGTG GTGGTCTGGT TCAGCCGGGT GGTTCTCTGC GTCTGTCTTG 3661 CGCTGCTTCT GGTAAAATGT CTTCTCGTCG TTGCATGGCT TGGTTCCGTC AGGCTCCGGG 3721 TAAAGAACGT GAACGTGTTG CTAAACTGCT GACCACCTCT GGTTCTACCT ACCTGGCTGA 3781 CTCTGTTAAA GGTCGTTTCA CCATCTCTCA GAACAACGCT AAATCTACCG TTTACCTGCA 3841 GATGAACTCT CTGAAACCGG AAGACACCGC TATGTACTAC TGCGCTGCTG ACTCTTTCGA 3901 AGACCCGACC TGCACCCTGG TTACCTCTTC TGGTGCTTTC CAGTACTGGG GTCAGGGTAC 3961 CCAGGTTACC GTTTCTGAAC CGAAATCTTG CGACAAAACC CACACCTGCC CGCCGTGCCC 4021 GGCTCCGGAA CTGCTGGGTG GTCCGTCTGT TTTCCTGTTC CCGCCGAAAC CGAAAGACAC 4081 CCTGATGATC TCTCGTACCC CGGAAGTTAC CTGCGTTGTT GTTGACGTTT CTCACGAAGA 4141 CCCGGAAGTT AAATTCAACT GGTACGTTGA CGGTGTTGAA GTTCACAACG CTAAAACCAA 4201 ACCGCGTGAA GAACAGTACA ACTCTACCTA CCGTGTTGTT TCTGTTCTGA CCGTTCTGCA 4261 CCAGGACTGG CTGAACGGTA AAGAATACAA ATGCAAAGTT TCTAACAAAG CTCTGCCGGC 4321 TCCGATCGAA AAAACCATCT CTAAAGCTAA AGGTCAGCCG CGTGAACCGC AGGTTTACAC 4381 CCTGCCGCCG TCTCGTGACG AACTGACCAA AAACCAGGTT TCTCTGACCT GCCTGGTTAA 4441 AGGTTTCTAC CCGTCTGACA TCGCTGTTGA ATGGGAATCT AACGGTCAGC CGGAAAACAA 4501 CTACAAAACC ACCCCGCCGG TTCTGGACTC TGACGGTTCT TTCTTCCTGT ACTCTAAACT 4561 GACCGTTGAC AAATCTCGTT GGCAGCAGGG TAACGTTTTC TCTTGCTCTG TTATGCACGA 4621 AGCTCTGCAC AACCACTACA CCCAGAAATC TCTGTCTCTG TCTCCGGGTA AAtagggatc 4681 cgcggccgcG aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc 4741 ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt 4801 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg 4861 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg 4921 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc 4981 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg 5041 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt 5101 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc 5161 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc 5221 tgtgaccggc gcctacgcta gacgccacca tggtgagcaa gggcgaggag gataacatgg 5281 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg 5341 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc 5401 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca 5461 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt 5521 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga 5581 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg 5641 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct 5701 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga 5761 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc 5821 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg 5881 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca 5941 tggacgagct gtacaaggga tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg 6001 tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc gccacccgcg 6061 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc 6121 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc 6181 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg 6241 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc 6301 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg 6361 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc 6421 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg 6481 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc 6541 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga 6601 cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa agattgactg 6661 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 6721 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 6781 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 6841 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 6901 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 6961 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga 7021 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 7081 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 7141 tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttggccgc 7201 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact 7261 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc 7321 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct 7381 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt 7441 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt 7501 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa 7561 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat 7621 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 7681 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac 7741 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 7801 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 7861 cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg 7921 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 7981 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 8041 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 8101 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 8161 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 8221 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 8281 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 8341 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 8401 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 8461 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 8521 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 8581 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 8641 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 8701 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 8761 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 8821 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 8881 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 8941 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 9001 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 9061 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 9121 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 9181 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 9241 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 9301 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 9361 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 9421 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 9481 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 9541 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 9601 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 9661 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 9721 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 9781 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 9841 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 9901 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 9961 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 10021 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 10081 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 10141 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 10201 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 10261 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 10321 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg 10381 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 10441 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 10501 taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.