pLV3-CMV-TSSK3-3×Myc-CopGFP-Pu

pLV3-CMV-TSSK3-3×Myc-CopGFP-Puro plasmid

PVT96358

pLV3-CMV-TSSK3-3×Myc-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-TSSK3(human)-3×Myc-CopGFP-Puro
Alias: NM_052841.4;SPOGA3; STK22C; STK22D; TSK3
Gene lenght: 804bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TSSK3-3×Myc-CopGFP-Puro plasmid Description

pLV3-CMV-TSSK3-3×Myc-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TSSK3-3×Myc-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgg aggactttct gctctccaat gggtaccagc tgggcaagac 2341 cattggggaa gggacctact caaaagtcaa agaagcattt tccaaaaaac accaaagaaa 2401 agtggcaatt aaagttatag acaagatggg agggccagaa gagtttatcc agagattcct 2461 ccctcgggag ctccaaatcg tccgtaccct ggaccacaag aacatcatcc aggtgtatga 2521 gatgctggag tctgccgacg ggaaaatctg cctggtgatg gagctcgctg agggagggga 2581 tgtctttgac tgcgtgctga atggggggcc actgcctgaa agccgggcca aggccctctt 2641 ccgtcagatg gttgaggcca tccgctactg ccatggctgt ggtgtggccc accgggacct 2701 caaatgtgag aacgccttgt tgcagggctt caacctgaag ctgactgact ttggctttgc 2761 caaggtgttg cccaagtcac accgggagct gagccagacc ttctgcggca gtacagccta 2821 tgctgccccc gaggtgctgc agggcattcc ccacgatagc aaaaaaggtg atgtctggag 2881 catgggtgtg gtcctgtatg tcatgctctg tgccagccta ccttttgacg acacagacat 2941 ccccaagatg ctgtggcagc agcagaaggg ggtgtccttc cccactcatc tgagcatctc 3001 ggccgattgc caggacctgc tcaagaggct cctggaaccc gatatgatcc tccggccttc 3061 aattgaagaa gttagttggc atccatggct agcaagcact ggatccGAAC AAAAACTAAT 3121 ATCAGAGGAA GATTTGGAAC AGAAACTAAT CAGTGAAGAA GATTTAGAGC AAAAGTTAAT 3181 TTCCGAGGAG GACTTATAgc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg 3241 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac 3301 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg 3361 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct 3421 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc 3481 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc 3541 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag 3601 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc 3661 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg 3721 ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga 3781 gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga 3841 gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat 3901 gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta 3961 cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat 4021 caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca 4081 cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg 4141 caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac 4201 cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt 4261 cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag 4321 cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga 4381 ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc 4441 catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg 4501 acccggctcc accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga 4561 ggagaatccc ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 4621 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 4681 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 4741 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt 4801 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 4861 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 4921 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca 4981 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 5041 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 5101 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 5161 caagcccggt gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 5221 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 5281 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 5341 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 5401 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 5461 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 5521 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 5581 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 5641 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 5701 gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg atctcccttt ggccgcctcc 5761 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 5821 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt 5881 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 5941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 6001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 6061 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 6121 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 6181 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 6241 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg 6301 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 6361 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 6421 gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat 6481 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 6541 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 6601 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 6661 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 6721 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 6781 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 6841 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 6901 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 6961 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 7021 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 7081 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 7141 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 7201 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 7261 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 7321 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 7381 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 7441 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 7501 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 7561 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 7621 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 7681 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 7741 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 7801 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 7861 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 7921 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 7981 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 8041 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 8101 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 8161 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 8221 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 8281 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 8341 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 8401 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 8461 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 8521 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 8581 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta 8641 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt 8701 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 8761 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt 8821 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 8881 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac 8941 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga 9001 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa 9061 acgacggcca gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.