pLV3-CMV-RET-3×Myc-CopGFP-Puro

pLV3-CMV-RET-3×Myc-CopGFP-Puro plasmid

PVT96353

pLV3-CMV-RET-3×Myc-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-RET(human)-3×Myc-CopGFP-Puro
Alias: NM_001406743.1;CDHF12; CDHR16; HSCR1; MEN2A; MEN2B; MTC1;PTC; RET-ELE1
Gene lenght: 3342bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-RET-3×Myc-CopGFP-Puro plasmid Description

pLV3-CMV-RET-3×Myc-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-RET-3×Myc-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgg cgaaggcgac gtccggtgcc gcggggctgc gtctgctgtt 2341 gctgctgctg ctgccgctgc taggcaaagt ggcattgggc ctctacttct cgagggatgc 2401 ttactgggag aagctgtatg tggaccaggc agccggcacg cccttgctgt acgtccatgc 2461 cctgcgggac gcccctgagg aggtgcccag cttccgcctg ggccagcatc tctacggcac 2521 gtaccgcaca cggctgcatg agaacaactg gatctgcatc caggaggaca ccggcctcct 2581 ctaccttaac cggagcctgg accatagctc ctgggagaag ctcagtgtcc gcaaccgcgg 2641 ctttcccctg ctcaccgtct acctcaaggt cttcctgtca cccacatccc ttcgtgaggg 2701 cgagtgccag tggccaggct gtgcccgcgt atacttctcc ttcttcaaca cctcctttcc 2761 agcctgcagc tccctcaagc cccgggagct ctgcttccca gagacaaggc cctccttccg 2821 cattcgggag aaccgacccc caggcacctt ccaccagttc cgcctgctgc ctgtgcagtt 2881 cttgtgcccc aacatcagcg tggcctacag gctcctggag ggtgagggtc tgcccttccg 2941 ctgcgccccg gacagcctgg aggtgagcac gcgctgggcc ctggaccgcg agcagcggga 3001 gaagtacgag ctggtggccg tgtgcaccgt gcacgccggc gcgcgcgagg aggtggtgat 3061 ggtgcccttc ccggtgaccg tgtacgacga ggacgactcg gcgcccacct tccccgcggg 3121 cgtcgacacc gccagcgccg tggtggagtt caagcggaag gaggacaccg tggtggccac 3181 gctgcgtgtc ttcgatgcag acgtggtacc tgcatcaggg gagctggtga ggcggtacac 3241 aagcacgctg ctccccgggg acacctgggc ccagcagacc ttccgggtgg aacactggcc 3301 caacgagacc tcggtccagg ccaacggcag cttcgtgcgg gcgaccgtac atgactatag 3361 gctggttctc aaccggaacc tctccatctc ggagaaccgc accatgcagc tggcggtgct 3421 ggtcaatgac tcagacttcc agggcccagg agcgggcgtc ctcttgctcc acttcaacgt 3481 gtcggtgctg ccggtcagcc tgcacctgcc cagtacctac tccctctccg tgagcaggag 3541 ggctcgccga tttgcccaga tcgggaaagt ctgtgtggaa aactgccagg cattcagtgg 3601 catcaacgtc cagtacaagc tgcattcctc tggtgccaac tgcagcacgc taggggtggt 3661 cacctcagcc gaggacacct cggggatcct gtttgtgaat gacaccaagg ccctgcggcg 3721 gcccaagtgt gccgaacttc actacatggt ggtggccacc gaccagcaga cctctaggca 3781 ggcccaggcc cagctgcttg taacagtgga ggggtcatat gtggccgagg aggcgggctg 3841 ccccctgtcc tgtgcagtca gcaagagacg gctggagtgt gaggagtgtg gcggcctggg 3901 ctccccaaca ggcaggtgtg agtggaggca aggagatggc aaagggatca ccaggaactt 3961 ctccacctgc tctcccagca ccaagacctg ccccgacggc cactgcgatg ttgtggagac 4021 ccaagacatc aacatttgcc ctcaggactg cctccggggc agcattgttg ggggacacga 4081 gcctggggag ccccggggga ttaaagctgg ctatggcacc tgcaactgct tccctgagga 4141 ggagaagtgc ttctgcgagc ccgaagacat ccaggatcca ctgtgcgacg agctgtgccg 4201 cacggtgatc gcagccgctg tcctcttctc cttcatcgtc tcggtgctgc tgtctgcctt 4261 ctgcatccac tgctaccaca agtttgccca caagccaccc atctcctcag ctgagatgac 4321 cttccggagg cccgcccagg ccttcccggt cagctactcc tcttccggtg cccgccggcc 4381 ctcgctggac tccatggaga accaggtctc cgtggatgcc ttcaagatcc tggaggatcc 4441 aaagtgggaa ttccctcgga agaacttggt tcttggaaaa actctaggag aaggcgaatt 4501 tggaaaagtg gtcaaggcaa cggccttcca tctgaaaggc agagcagggt acaccacggt 4561 ggccgtgaag atgctgaaag agaacgcctc cccgagtgag ctgcgagacc tgctgtcaga 4621 gttcaacgtc ctgaagcagg tcaaccaccc acatgtcatc aaattgtatg gggcctgcag 4681 ccaggatggc ccgctcctcc tcatcgtgga gtacgccaaa tacggctccc tgcggggctt 4741 cctccgcgag agccgcaaag tggggcctgg ctacctgggc agtggaggca gccgcaactc 4801 cagctccctg gaccacccgg atgagcgggc cctcaccatg ggcgacctca tctcatttgc 4861 ctggcagatc tcacagggga tgcagtatct ggccgagatg aagctcgttc atcgggactt 4921 ggcagccaga aacatcctgg tagctgaggg gcggaagatg aagatttcgg atttcggctt 4981 gtcccgagat gtttatgaag aggattccta cgtgaagagg agccagggtc ggattccagt 5041 taaatggatg gcaattgaat ccctttttga tcatatctac accacgcaaa gtgatgtatg 5101 gtcttttggt gtcctgctgt gggagatcgt gaccctaggg ggaaacccct atcctgggat 5161 tcctcctgag cggctcttca accttctgaa gaccggccac cggatggaga ggccagacaa 5221 ctgcagcgag gagatgtacc gcctgatgct gcaatgctgg aagcaggagc cggacaaaag 5281 gccggtgttt gcggacatca gcaaagacct ggagaagatg atggttaaga ggagagacta 5341 cttggacctt gcggcgtcca ctccatctga ctccctgatt tatgacgacg gcctctcaga 5401 ggaggagaca ccgctggtgg actgtaataa tgcccccctc cctcgagccc tcccttccac 5461 atggattgaa aacaaactct atggcatgtc agacccgaac tggcctggag agagtcctgt 5521 accactcacg agagctgatg gcactaacac tgggtttcca agatatccaa atgatagtgt 5581 atatgctaac tggatgcttt caccctcagc ggcaaaatta atggacacgt ttgatagtgg 5641 atccGAACAA AAACTAATAT CAGAGGAAGA TTTGGAACAG AAACTAATCA GTGAAGAAGA 5701 TTTAGAGCAA AAGTTAATTT CCGAGGAGGA CTTATAgcgg ccgcGaagga tctgcgatcg 5761 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg 5821 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga 5881 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt 5941 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg 6001 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg 6061 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta 6121 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc 6181 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt 6241 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc 6301 caccatggag agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg 6361 caccctgaac ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg 6421 ccgcatgacc aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct 6481 gagccacgtg atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa 6541 ccccttcctg cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga 6601 ggacggcggc gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg 6661 cgacttcaag gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat 6721 catccgcagc aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg 6781 cagcttcgcc cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag 6841 ccacatgcac ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt 6901 cgccttccgc cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca 6961 gcacgccttc aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc 7021 cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct 7081 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg 7141 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 7201 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 7261 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 7321 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 7381 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 7441 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 7501 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 7561 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 7621 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 7681 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg 7741 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 7801 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 7861 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 7921 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 7981 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 8041 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 8101 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 8161 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 8221 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat 8281 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 8341 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 8401 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 8461 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 8521 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 8581 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 8641 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 8701 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 8761 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 8821 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 8881 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 8941 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag 9001 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 9061 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 9121 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 9181 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 9241 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 9301 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 9361 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 9421 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 9481 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 9541 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 9601 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 9661 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 9721 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 9781 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 9841 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 9901 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 9961 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 10021 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 10081 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 10141 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 10201 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 10261 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 10321 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 10381 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 10441 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 10501 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 10561 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 10621 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 10681 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 10741 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 10801 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 10861 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 10921 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 10981 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 11041 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 11101 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 11161 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg 11221 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 11281 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 11341 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 11401 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg 11461 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 11521 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 11581 cagtcacgac gttgtaaaac gacggccagt gccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.