pLV3-EF1a-PPM1K-3×FLAG-Puro pl

pLV3-EF1a-PPM1K-3×FLAG-Puro plasmid

PVT96023

pLV3-EF1a-PPM1K-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-EF1a-PPM1K(human)-3×FLAG-Puro
Alias: NM_152542.5;BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP;UG0882E07
Gene lenght: 1116bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F-72
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-PPM1K-3×FLAG-Puro plasmid Description

pLV3-EF1a-PPM1K-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-PPM1K-3×FLAG-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cGCCACCatg tcaacagctg ccttaattac 2521 tttggtcaga agtggtggga accaggtgag aaggagagtg ctgctaagct cccgcctgct 2581 gcaggacgac aggcgggtga cacccacgtg ccacagctcc acttcagagc ctaggtgttc 2641 tcggtttgac ccagatggta gtgggagtcc agctacctgg gacaattttg ggatctggga 2701 taaccgcatt gatgagccaa ttctgctgcc acccagcatt aagtatggca agccaattcc 2761 caaaatcagc ttggaaaatg tggggtgcgc ctcacagatt ggcaaacgga aagagaatga 2821 agatcggttt gacttcgctc agctgacaga tgaggtcctg tactttgcag tgtatgatgg 2881 acacggtgga cctgcagcag ctgatttctg tcatacccac atggagaaat gtattatgga 2941 tttgcttcct aaggagaaga acttggaaac tctgttgacc ttggcttttc tagaaataga 3001 taaagccttt tcgagtcatg cccgcctgtc tgctgatgca actcttctga cctctgggac 3061 tactgcaaca gtagccctat tgcgagatgg tattgaactg gttgtagcca gtgttgggga 3121 cagccgggct attttgtgta gaaaaggaaa acccatgaag ctgaccattg accatactcc 3181 agaaagaaaa gatgaaaaag aaaggatcaa gaaatgtggt ggttttgtag cttggaatag 3241 tttggggcag cctcacgtaa atggcaggct tgcaatgaca agaagtattg gagatttgga 3301 ccttaagacc agtggtgtca tagcagaacc tgaaactaag aggattaagt tacatcatgc 3361 tgatgacagc ttcctggtcc tcaccacaga tggaattaac ttcatggtga atagtcaaga 3421 gatttgtgac tttgtcaatc agtgccatga tcccaacgaa gcagcccatg cggtgactga 3481 acaggcaata cagtacggta ctgaggataa cagtactgca gtagtagtgc cttttggtgc 3541 ctggggaaaa tataagaact ctgaaatcaa cttctcattc agcagaagct ttgcctccag 3601 tggacgatgg gccCTCGAGG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT 3661 CGACTACAAG GATGACGATG ACAAGgcggc cgctgagggc agaggaagtc ttctaacatg 3721 cggtgacgtg gaggagaatc ccggcccttc cgggatgacc gagtacaagc ccacggtgcg 3781 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 3841 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 3901 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 3961 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 4021 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 4081 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 4141 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 4201 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 4261 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 4321 cacctggtgc atgacccgca agcccggtgc ctgaatctag gtcgacaatc aacctctgga 4381 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 4441 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 4501 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 4561 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 4621 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 4681 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 4741 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac 4801 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 4861 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 4921 gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt 4981 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 5041 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 5101 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 5161 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 5221 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 5281 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 5341 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 5401 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 5461 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 5521 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 5581 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta 5641 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 5701 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 5761 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 5821 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 5881 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 5941 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 6001 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 6061 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 6121 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 6181 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 6241 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 6301 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 6361 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 6421 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 6481 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 6541 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 6601 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 6661 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 6721 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 6781 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 6841 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 6901 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 6961 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 7021 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 7081 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 7141 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 7201 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 7261 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 7321 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 7381 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 7441 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 7501 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 7561 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 7621 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 7681 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 7741 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 7801 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 7861 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 7921 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 7981 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 8041 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 8101 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 8161 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 8221 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.