pLV3-CMV-PDGFRA-3×Myc-EF1a-Cop

pLV3-CMV-PDGFRA-3×Myc-EF1a-CopGFP-Puro plasmid

PVT96340

pLV3-CMV-PDGFRA-3×Myc-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-PDGFRA(human)-3×Myc-EF1a-CopGFP-Puro
Alias: NM_006206.6;CD140A; PDGFR-2; PDGFR2
Gene lenght: 3267bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PDGFRA-3×Myc-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-PDGFRA-3×Myc-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-PDGFRA-3×Myc-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc gccaccatgg ggacttccca tccggcgttc ctggtcttag gctgtcttct 2341 cacagggctg agcctaatcc tctgccagct ttcattaccc tctatccttc caaatgaaaa 2401 tgaaaaggtt gtgcagctga attcatcctt ttctctgaga tgctttgggg agagtgaagt 2461 gagctggcag taccccatgt ctgaagaaga gagctccgat gtggaaatca gaaatgaaga 2521 aaacaacagc ggcctttttg tgacggtctt ggaagtgagc agtgcctcgg cggcccacac 2581 agggttgtac acttgctatt acaaccacac tcagacagaa gagaatgagc ttgaaggcag 2641 gcacatttac atctatgtgc cagacccaga tgtagccttt gtacctctag gaatgacgga 2701 ttatttagtc atcgtggagg atgatgattc tgccattata ccttgtcgca caactgatcc 2761 cgagactcct gtaaccttac acaacagtga gggggtggta cctgcctcct acgacagcag 2821 acagggcttt aatgggacct tcactgtagg gccctatatc tgtgaggcca ccgtcaaagg 2881 aaagaagttc cagaccatcc catttaatgt ttatgcttta aaagcaacat cagagctgga 2941 tctagaaatg gaagctctta aaaccgtgta taagtcaggg gaaacgattg tggtcacctg 3001 tgctgttttt aacaatgagg tggttgacct tcaatggact taccctggag aagtgaaagg 3061 caaaggcatc acaatgctgg aagaaatcaa agtcccatcc atcaaattgg tgtacacttt 3121 gacggtcccc gaggccacgg tgaaagacag tggagattac gaatgtgctg cccgccaggc 3181 taccagggag gtcaaagaaa tgaagaaagt cactatttct gtccatgaga aaggtttcat 3241 tgaaatcaaa cccaccttca gccagttgga agctgtcaac ctgcatgaag tcaaacattt 3301 tgttgtagag gtgcgggcct acccacctcc caggatatcc tggctgaaaa acaatctgac 3361 tctgattgaa aatctcactg agatcaccac tgatgtggaa aagattcagg aaataaggta 3421 tcgaagcaaa ttaaagctga tccgtgctaa ggaagaagac agtggccatt atactattgt 3481 agctcaaaat gaagatgctg tgaagagcta tacttttgaa ctgttaactc aagttccttc 3541 atccattctg gacttggtcg atgatcacca tggctcaact gggggacaga cggtgaggtg 3601 cacagctgaa ggcacgccgc ttcctgatat tgagtggatg atatgcaaag atattaagaa 3661 atgtaataat gaaacttcct ggactatttt ggccaacaat gtctcaaaca tcatcacgga 3721 gatccactcc cgagacagga gtaccgtgga gggccgtgtg actttcgcca aagtggagga 3781 gaccatcgcc gtgcgatgcc tggctaagaa tctccttgga gctgagaacc gagagctgaa 3841 gctggtggct cccaccctgc gttctgaact cacggtggct gctgcagtcc tggtgctgtt 3901 ggtgattgtg atcatctcac ttattgtcct ggttgtcatt tggaaacaga aaccgaggta 3961 tgaaattcgc tggagggtca ttgaatcaat cagcccagat ggacatgaat atatttatgt 4021 ggacccgatg cagctgcctt atgactcaag atgggagttt ccaagagatg gactagtgct 4081 tggtcgggtc ttggggtctg gagcgtttgg gaaggtggtt gaaggaacag cctatggatt 4141 aagccggtcc caacctgtca tgaaagttgc agtgaagatg ctaaaaccca cggccagatc 4201 cagtgaaaaa caagctctca tgtctgaact gaagataatg actcacctgg ggccacattt 4261 gaacattgta aacttgctgg gagcctgcac caagtcaggc cccatttaca tcatcacaga 4321 gtattgcttc tatggagatt tggtcaacta tttgcataag aatagggata gcttcctgag 4381 ccaccaccca gagaagccaa agaaagagct ggatatcttt ggattgaacc ctgctgatga 4441 aagcacacgg agctatgtta ttttatcttt tgaaaacaat ggtgactaca tggacatgaa 4501 gcaggctgat actacacagt atgtccccat gctagaaagg aaagaggttt ctaaatattc 4561 cgacatccag agatcactct atgatcgtcc agcctcatat aagaagaaat ctatgttaga 4621 ctcagaagtc aaaaacctcc tttcagatga taactcagaa ggccttactt tattggattt 4681 gttgagcttc acctatcaag ttgcccgagg aatggagttt ttggcttcaa aaaattgtgt 4741 ccaccgtgat ctggctgctc gcaacgtcct cctggcacaa ggaaaaattg tgaagatctg 4801 tgactttggc ctggccagag acatcatgca tgattcgaac tatgtgtcga aaggcagtac 4861 ctttctgccc gtgaagtgga tggctcctga gagcatcttt gacaacctct acaccacact 4921 gagtgatgtc tggtcttatg gcattctgct ctgggagatc ttttcccttg gtggcacccc 4981 ttaccccggc atgatggtgg attctacttt ctacaataag atcaagagtg ggtaccggat 5041 ggccaagcct gaccacgcta ccagtgaagt ctacgagatc atggtgaaat gctggaacag 5101 tgagccggag aagagaccct ccttttacca cctgagtgag attgtggaga atctgctgcc 5161 tggacaatat aaaaagagtt atgaaaaaat tcacctggac ttcctgaaga gtgaccatcc 5221 tgctgtggca cgcatgcgtg tggactcaga caatgcatac attggtgtca cctacaaaaa 5281 cgaggaagac aagctgaagg actgggaggg tggtctggat gagcagagac tgagcgctga 5341 cagtggctac atcattcctc tgcctgacat tgaccctgtc cctgaggagg aggacctggg 5401 caagaggaac agacacagct cgcagacctc tgaagagagt gccattgaga cgggttccag 5461 cagttccacc ttcatcaaga gagaggacga gaccattgaa gacatcgaca tgatggatga 5521 catcggcata gactcttcag acctggtgga agacagcttc ctgggatccG AACAAAAACT 5581 AATATCAGAG GAAGATTTGG AACAGAAACT AATCAGTGAA GAAGATTTAG AGCAAAAGTT 5641 AATTTCCGAG GAGGACTTAT AgcggccgcG aaggatctgc gatcgctccg gtgcccgtca 5701 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 5761 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 5821 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 5881 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc 5941 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct 6001 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct 6061 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct 6121 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct 6181 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga 6241 cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt 6301 ggagttcgag ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa 6361 gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg 6421 ctacggcttc taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc 6481 catcaacaac ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct 6541 gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt 6601 gggcaccggc ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc 6661 caccgtggag cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac 6721 cttcagcctg cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa 6781 gagcgccatc caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt 6841 ggaggagctg cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac 6901 ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc 6961 cggacccggc tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt 7021 ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 7081 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 7141 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 7201 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 7261 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 7321 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 7381 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 7441 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 7501 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 7561 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 7621 ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg 7681 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 7741 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 7801 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 7861 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 7921 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 7981 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 8041 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 8101 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 8161 gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc 8221 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 8281 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct 8341 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 8401 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 8461 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 8521 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 8581 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 8641 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 8701 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact 8761 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 8821 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 8881 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga 8941 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 9001 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 9061 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 9121 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 9181 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 9241 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 9301 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 9361 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 9421 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 9481 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 9541 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 9601 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 9661 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 9721 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 9781 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 9841 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 9901 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 9961 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 10021 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 10081 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 10141 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 10201 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 10261 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 10321 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 10381 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 10441 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 10501 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 10561 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 10621 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 10681 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 10741 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 10801 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 10861 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 10921 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 10981 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 11041 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 11101 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac 11161 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 11221 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg 11281 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 11341 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc 11401 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg 11461 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt 11521 aaaacgacgg ccagtgccaa gctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.