pCMV-APP-WPRE-Neo plasmid

pCMV-APP-WPRE-Neo plasmid

PVT90805

pCMV-APP-WPRE-Neo plasmid Information

Plasmid name: pCMV-APP(human)-WPRE-Neo
Alias: NM_201414.3;AAA; ABETA; ABPP; AD1; alpha-sAPP; APPI;CTFgamma; CVAP; PN-II; PN2; preA4
Gene lenght: 2088bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-APP-WPRE-Neo plasmid Description

pCMV-APP-WPRE-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-APP-WPRE-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG 661 AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGG 721 ACTCTAGAGC TAGCGTTTAA ACTTAAGCTT GGTACCGAGC TCGGATCCGC CACCATGCTG 781 CCCGGTTTGG CACTGCTCCT GCTGGCCGCC TGGACGGCTC GGGCGCTGGA GGTACCCACT 841 GATGGTAATG CTGGCCTGCT GGCTGAACCC CAGATTGCCA TGTTCTGTGG CAGACTGAAC 901 ATGCACATGA ATGTCCAGAA TGGGAAGTGG GATTCAGATC CATCAGGGAC CAAAACCTGC 961 ATTGATACCA AGGAAGGCAT CCTGCAGTAT TGCCAAGAAG TCTACCCTGA ACTGCAGATC 1021 ACCAATGTGG TAGAAGCCAA CCAACCAGTG ACCATCCAGA ACTGGTGCAA GCGGGGCCGC 1081 AAGCAGTGCA AGACCCATCC CCACTTTGTG ATTCCCTACC GCTGCTTAGT TGGTGAGTTT 1141 GTAAGTGATG CCCTTCTCGT TCCTGACAAG TGCAAATTCT TACACCAGGA GAGGATGGAT 1201 GTTTGCGAAA CTCATCTTCA CTGGCACACC GTCGCCAAAG AGACATGCAG TGAGAAGAGT 1261 ACCAACTTGC ATGACTACGG CATGTTGCTG CCCTGCGGAA TTGACAAGTT CCGAGGGGTA 1321 GAGTTTGTGT GTTGCCCACT GGCTGAAGAA AGTGACAATG TGGATTCTGC TGATGCGGAG 1381 GAGGATGACT CGGATGTCTG GTGGGGCGGA GCAGACACAG ACTATGCAGA TGGGAGTGAA 1441 GACAAAGTAG TAGAAGTAGC AGAGGAGGAA GAAGTGGCTG AGGTGGAAGA AGAAGAAGCC 1501 GATGATGACG AGGACGATGA GGATGGTGAT GAGGTAGAGG AAGAGGCTGA GGAACCCTAC 1561 GAAGAAGCCA CAGAGAGAAC CACCAGCATT GCCACCACCA CCACCACCAC CACAGAGTCT 1621 GTGGAAGAGG TGGTTCGAGT TCCTACAACA GCAGCCAGTA CCCCTGATGC CGTTGACAAG 1681 TATCTCGAGA CACCTGGGGA TGAGAATGAA CATGCCCATT TCCAGAAAGC CAAAGAGAGG 1741 CTTGAGGCCA AGCACCGAGA GAGAATGTCC CAGGTCATGA GAGAATGGGA AGAGGCAGAA 1801 CGTCAAGCAA AGAACTTGCC TAAAGCTGAT AAGAAGGCAG TTATCCAGCA TTTCCAGGAG 1861 AAAGTGGAAT CTTTGGAACA GGAAGCAGCC AACGAGAGAC AGCAGCTGGT GGAGACACAC 1921 ATGGCCAGAG TGGAAGCCAT GCTCAATGAC CGCCGCCGCC TGGCCCTGGA GAACTACATC 1981 ACCGCTCTGC AGGCTGTTCC TCCTCGGCCT CGTCACGTGT TCAATATGCT AAAGAAGTAT 2041 GTCCGCGCAG AACAGAAGGA CAGACAGCAC ACCCTAAAGC ATTTCGAGCA TGTGCGCATG 2101 GTGGATCCCA AGAAAGCCGC TCAGATCCGG TCCCAGGTTA TGACACACCT CCGTGTGATT 2161 TATGAGCGCA TGAATCAGTC TCTCTCCCTG CTCTACAACG TGCCTGCAGT GGCCGAGGAG 2221 ATTCAGGATG AAGTTGATGA GCTGCTTCAG AAAGAGCAAA ACTATTCAGA TGACGTCTTG 2281 GCCAACATGA TTAGTGAACC AAGGATCAGT TACGGAAACG ATGCTCTCAT GCCATCTTTG 2341 ACCGAAACGA AAACCACCGT GGAGCTCCTT CCCGTGAATG GAGAGTTCAG CCTGGACGAT 2401 CTCCAGCCGT GGCATTCTTT TGGGGCTGAC TCTGTGCCAG CCAACACAGA AAACGAAGTT 2461 GAGCCTGTTG ATGCCCGCCC TGCTGCCGAC CGAGGACTGA CCACTCGACC AGGTTCTGGG 2521 TTGACAAATA TCAAGACGGA GGAGATCTCT GAAGTGAATC TGGATGCAGA ATTCCGACAT 2581 GACTCAGGAT ATGAAGTTCA TCATCAAAAA TTGGTGTTCT TTGCAGAAGA TGTGGGTTCA 2641 AACAAAGGTG CAATCATTGG ACTCATGGTG GGCGGTGTTG TCATAGCGAC AGTGATCGTC 2701 ATCACCTTGG TGATGCTGAA GAAGAAACAG TACACATCCA TTCATCATGG TGTGGTGGAG 2761 GTTGACGCCG CTGTCACCCC AGAGGAGCGC CACCTGTCCA AGATGCAGCA GAACGGCTAC 2821 GAAAATCCAA CCTACAAGTT CTTTGAGCAG ATGCAGAACT AGCTCGAGTC TAGAGGGCCC 2881 CTTAAGGGTT CGATCCCTAC CGGTTAGTAA TGAGTTTGAT ATCTCGACAA TCAACCTCTG 2941 GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA 3001 TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT 3061 TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC 3121 AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT 3181 GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG 3241 GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC 3301 AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC 3361 ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC 3421 CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT 3481 CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GAAACGGGGG AGGCTAACTG 3541 AAACACGGAA GGAGACAATA CCGGAAGGAA CCCGCGCTAT GACGGCAATA AAAAGACAGA 3601 ATAAAACGCA CGGGTGTTGG GTCGTTTGTT CATAAACGCG GGGTTCGGTC CCAGGGCTGG 3661 CACTCTGTCG ATACCCCACC GAGACCCCAT TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT 3721 TCCCCACCCC ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG 3781 GCAGGCCCTG CCATAGCAGA TCTGCGCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC 3841 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 3901 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 3961 GGCTTTCCCC GTCAAGCTCT AAATCGGGGC ATCCCTTTAG GGTTCCGATT TAGTGCTTTA 4021 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 4081 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 4141 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 4201 TTGGGGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 4261 TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA 4321 GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT 4381 CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC 4441 CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG 4501 GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC 4561 AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT 4621 GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC 4681 AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT 4741 GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC 4801 GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG 4861 CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG 4921 TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT 4981 CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC 5041 ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG 5101 CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG 5161 GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC 5221 TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT 5281 CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG 5341 CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT 5401 ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT 5461 TCTGAGCGGG ACTCTGGGGT TCGCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA 5521 CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG 5581 GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC 5641 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA 5701 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT 5761 TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG 5821 TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA 5881 AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA 5941 CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC 6001 GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG 6061 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA 6121 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 6181 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG 6241 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC 6301 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC 6361 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT 6421 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 6481 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA 6541 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA 6601 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA 6661 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA 6721 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 6781 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 6841 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC 6901 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT 6961 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT 7021 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA 7081 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA 7141 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC 7201 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT 7261 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT 7321 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG 7381 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA 7441 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA 7501 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG 7561 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT 7621 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG 7681 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT 7741 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA 7801 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC 7861 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 7921 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA 7981 GATCTCCCGA TCCCCTATGG TCGACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA 8041 GCCAGTATCT GCTCCCTGCT TGTGTGTTGG AGGTCGCTGA GTAGTGCGCG AGCAAAATTT 8101 AAGCTACAAC AAGGCAAGGC TTGACCGACA ATTGCATGAA GAATCTGCTT AGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.