pLV3-CMV-Hlf-3×Myc-EF1a-CopGFP

pLV3-CMV-Hlf-3×Myc-EF1a-CopGFP-Puro plasmid

PVT96857

pLV3-CMV-Hlf-3×Myc-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Hlf(mouse)-3×Myc-EF1a-CopGFP-Puro
Alias: NM_172563.4;E230015K02Rik
Gene lenght: 888bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Hlf-3×Myc-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-Hlf-3×Myc-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Hlf-3×Myc-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgg agaaaatgtc ccgacagctc cccttgaacc ccacctttat 2341 cccgcctccc tacggcgtgc tcaggtccct gctggagaac ccgctgaagc tcccccttca 2401 tcctgaagac gcatttagta aagaaaaaga caaaggaaag aagctggacg atgagagcag 2461 cagcccgacc gtcccccagt ccgccttctt gggacccacc ttatgggaca aaacccttcc 2521 ctatgacgga gatactttcc agctggaata catggacttg gaggaattcc tgtcagagaa 2581 tggcatcccc ccgagtccgt cgcagcacga ccacagccct cacccccctg gcttgcaacc 2641 agcttcctcc acggcgccct cggtcatgga tctcagcagc cgggccacag cgcccctcca 2701 cccgggcatc ccgtctccga actgtatgca gagccccatc agaccaggcc agctgttgcc 2761 agcaaaccgc aatacaccga gtcccattga ccctgacacc atccaggtac cagtcgggta 2821 tgaaccagac ccggcagacc ttgccctctc cagcatcccg gggcaggaaa tgtttgaccc 2881 tcgcaaacgg aagttctctg aggaagaact gaagccacag cccatgatta agaaagctcg 2941 caaagtcttc attcccgatg atttgaagga tgacaagtac tgggcgaggc gcagaaagaa 3001 caacatggcg gccaagcgct cccgtgatgc ccggcggctg aaggagaacc agatcgcaat 3061 ccgggcctca ttcctggaga aggagaactc ggccctccgc caggaggtgg ctgatttaag 3121 gaaggagctg ggcaaatgca agaacatact tgccaagtac gaggccaggc acgggcccct 3181 gggatccGAA CAAAAACTAA TATCAGAGGA AGATTTGGAA CAGAAACTAA TCAGTGAAGA 3241 AGATTTAGAG CAAAAGTTAA TTTCCGAGGA GGACTTATAg cggccgcGaa ggatctgcga 3301 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 3361 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag 3421 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 3481 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct 3541 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg 3601 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt 3661 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct 3721 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt 3781 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga 3841 cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac 3901 cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca 3961 gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct 4021 gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga 4081 gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta 4141 cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat 4201 cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa 4261 gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt 4321 gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga 4381 cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat 4441 gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta 4501 ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc 4561 tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct 4621 tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt 4681 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc 4741 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga 4801 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga 4861 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt 4921 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca 4981 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt 5041 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt 5101 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct 5161 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc 5221 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat 5281 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 5341 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 5401 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 5461 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 5521 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 5581 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 5641 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 5701 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 5761 cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg 5821 gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 5881 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 5941 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 6001 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 6061 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 6121 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 6181 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 6241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 6301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 6361 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 6421 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 6481 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca 6541 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 6601 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 6661 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 6721 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 6781 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 6841 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 6901 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 6961 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 7021 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 7081 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 7141 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 7201 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 7261 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 7321 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 7381 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 7441 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 7501 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 7561 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 7621 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 7681 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 7741 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 7801 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 7861 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 7921 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 7981 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 8041 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 8101 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 8161 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 8221 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 8281 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 8341 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 8401 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 8461 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 8521 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 8581 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 8641 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 8701 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 8761 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 8821 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 8881 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac 8941 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat 9001 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 9061 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 9121 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.