pLV3-CMV-Atf3-3×FLAG-CopGFP-Ne

pLV3-CMV-Atf3-3×FLAG-CopGFP-Neo plasmid

PVT96292

pLV3-CMV-Atf3-3×FLAG-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-Atf3(mouse)-3×FLAG-CopGFP-Neo
Alias: NM_007498.3;LRG-21
Gene lenght: 543bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Atf3-3×FLAG-CopGFP-Neo plasmid Description

pLV3-CMV-Atf3-3×FLAG-CopGFP-Neo It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Atf3-3×FLAG-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgatgct tcaacatcca ggccaggtct ctgcctcaga 2341 agtcagtgcg accgccattg tcccctgcct ctcacctcct gggtcactgg tatttgagga 2401 ttttgctaac ctgacaccct ttgtcaagga agagctgaga ttcgccatcc agaataaaca 2461 cctctgccat cggatgtcct ctgcgctgga gtcagttacc gtcaacaaca gacccctgga 2521 gatgtcagtc accaagtctg aggcggcccc tgaagaagat gagaggaaaa ggaggcggcg 2581 agaaagaaat aaaattgctg ctgccaagtg tcgaaacaag aaaaaggaga agacagagtg 2641 cctgcagaaa gagtcagaga aactggagag tgtgaatgct gagctgaagg cccagattga 2701 ggagctgaag aatgagaaac agcatttgat atacatgctc aacctgcacc ggcccacctg 2761 catcgtccgg gctcagaatg gacggacacc ggaagacgag aggaacctct ttatccaaca 2821 gataaaagaa ggaacattgc agagcggatc cGACTACAAA GACCATGACG GTGATTATAA 2881 AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGTAg cggccgcGaa ggatctgcga 2941 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 3001 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag 3061 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 3121 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct 3181 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg 3241 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt 3301 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct 3361 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt 3421 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga 3481 cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac 3541 cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca 3601 gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct 3661 gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga 3721 gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta 3781 cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat 3841 cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa 3901 gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt 3961 gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga 4021 cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat 4081 gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta 4141 ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc 4201 tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct 4261 tctaacatgc ggtgacgtgg aggagaatcc cggccctGCC ACCatggctg aacaagatgg 4321 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 4381 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 4441 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 4501 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 4561 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 4621 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 4681 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 4741 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 4801 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 4861 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 4921 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 4981 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 5041 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaGT 5101 CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT 5161 TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC 5221 CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA 5281 GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC 5341 CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT 5401 CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG 5461 GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT 5521 GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC 5581 CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG 5641 TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCggtacct 5701 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 5761 ggactggaag ggctaattca ctcccaacga aGataagatc tgctttttgc ttgtactggg 5821 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 5881 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 5941 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 6001 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 6061 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 6121 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 6181 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc 6241 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 6301 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag 6361 acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 6421 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 6481 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 6541 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 6601 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 6661 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 6721 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 6781 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 6841 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 6901 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 6961 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 7021 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 7081 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 7141 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7201 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 7261 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 7321 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 7381 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 7441 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 7501 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 7561 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 7621 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 7681 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 7741 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 7801 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 7861 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 7921 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 7981 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 8041 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 8101 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 8161 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 8221 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 8281 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 8341 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 8401 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 8461 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 8521 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 8581 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac 8641 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 8701 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat 8761 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 8821 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 8881 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 8941 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 9001 ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.