pLV3-CMV-PRKCD-3×Myc-mCherry-P

pLV3-CMV-PRKCD-3×Myc-mCherry-Puro plasmid

PVT96287

pLV3-CMV-PRKCD-3×Myc-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-PRKCD(human)-3×Myc-mCherry-Puro
Alias: NM_006254.4;ALPS3; CVID9; MAY1; nPKC-delta; PKCD
Gene lenght: 2028bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PRKCD-3×Myc-mCherry-Puro plasmid Description

pLV3-CMV-PRKCD-3×Myc-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-PRKCD-3×Myc-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggcgcc gttcctgcgc atcgccttca actcctatga 2341 gctgggctcc ctgcaggccg aggacgaggc gaaccagccc ttctgtgccg tgaagatgaa 2401 ggaggcgctc agcacagagc gtgggaaaac actggtgcag aagaagccga ccatgtatcc 2461 tgagtggaag tcgacgttcg atgcccacat ctatgagggg cgcgtcatcc agattgtgct 2521 aatgcgggca gcagaggagc cagtgtctga ggtgaccgtg ggtgtgtcgg tgctggccga 2581 gcgctgcaag aagaacaatg gcaaggctga gttctggctg gacctgcagc ctcaggccaa 2641 ggtgttgatg tctgttcagt atttcctgga ggacgtggat tgcaaacagt ctatgcgcag 2701 tgaggacgag gccaagttcc caacgatgaa ccgccgcgga gccatcaaac aggccaaaat 2761 ccactacatc aagaaccatg agtttatcgc caccttcttt gggcaaccca ccttctgttc 2821 tgtgtgcaaa gactttgtct ggggcctcaa caagcaaggc tacaaatgca ggcaatgtaa 2881 cgctgccatc cacaagaaat gcatcgacaa gatcatcggc agatgcactg gcaccgcggc 2941 caacagccgg gacactatat tccagaaaga acgcttcaac atcgacatgc cgcaccgctt 3001 caaggttcac aactacatga gccccacctt ctgtgaccac tgcggcagcc tgctctgggg 3061 actggtgaag cagggattaa agtgtgaaga ctgcggcatg aatgtgcacc ataaatgccg 3121 ggagaaggtg gccaacctct gcggcatcaa ccagaagctt ttggctgagg ccttgaacca 3181 agtcacccag agagcctccc ggagatcaga ctcagcctcc tcagagcctg ttgggatata 3241 tcagggtttc gagaagaaga ccggagttgc tggggaggac atgcaagaca acagtgggac 3301 ctacggcaag atctgggagg gcagcagcaa gtgcaacatc aacaacttca tcttccacaa 3361 ggtcctgggc aaaggcagct tcgggaaggt gctgcttgga gagctgaagg gcagaggaga 3421 gtactttgcc atcaaggccc tcaagaagga tgtggtcctg atcgacgacg acgtggagtg 3481 caccatggtt gagaagcggg tgctgacact tgccgcagag aatccctttc tcacccacct 3541 catctgcacc ttccagacca aggaccacct gttctttgtg atggagttcc tcaacggggg 3601 ggacctgatg taccacatcc aggacaaagg ccgctttgaa ctctaccgtg ccacgtttta 3661 tgccgctgag ataatgtgtg gactgcagtt tctacacagc aagggcatca tttacaggga 3721 cctcaaactg gacaatgtgc tgctggaccg ggatggccac atcaagattg ccgactttgg 3781 gatgtgcaaa gagaacatat tcggggagag ccgggccagc accttctgcg gcacccctga 3841 ctatatcgcc cctgagatcc tacagggcct gaagtacaca ttctctgtgg actggtggtc 3901 tttcggggtc cttctgtacg agatgctcat tggccagtcc cccttccatg gtgatgatga 3961 ggatgaactc ttcgagtcca tccgtgtgga cacgccacat tatccccgct ggatcaccaa 4021 ggagtccaag gacatcctgg agaagctctt tgaaagggaa ccaaccaaga ggctgggagt 4081 gaccggaaac atcaaaatcc accccttctt caagaccata aactggactc tgctggaaaa 4141 gcggaggttg gagccacctt tcaggcccaa agtgaagtca cccagagact acagtaactt 4201 tgaccaggag ttcctgaacg agaaggcgcg cctctcctac agcgacaaga acctcatcga 4261 ctccatggac cagtctgcat tcgctggctt ctcctttgtg aaccccaaat tcgagcacct 4321 cctggaagat ctcgagGAAC AAAAACTAAT ATCAGAGGAA GATTTGGAAC AGAAACTAAT 4381 CAGTGAAGAA GATTTAGAGC AAAAGTTAAT TTCCGAGGAG GACTTAtagg atccgcggcc 4441 gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 4501 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg 4561 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 4621 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 4681 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg 4741 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg 4801 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc 4861 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc 4921 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc 4981 ggcgcctacg ctagacgcca ccatggtgag caagggcgag gaggataaca tggccatcat 5041 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga 5101 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt 5161 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg 5221 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc 5281 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac 5341 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa 5401 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga 5461 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa 5521 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca 5581 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta 5641 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga 5701 gctgtacaag ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga 5761 gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 5821 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 5881 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 5941 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 6001 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 6061 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 6121 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 6181 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 6241 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 6301 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 6361 gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 6421 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 6481 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 6541 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 6601 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 6661 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 6721 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 6781 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 6841 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 6901 tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg 6961 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 7021 agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc 7081 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 7141 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 7201 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 7261 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 7321 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 7381 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 7441 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 7501 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 7561 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 7621 ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 7681 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 7741 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 7801 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 7861 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 7921 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 7981 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 8041 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 8101 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 8161 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 8221 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 8281 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 8341 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 8401 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 8461 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 8521 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 8581 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 8641 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 8701 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 8761 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 8821 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 8881 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 8941 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 9001 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 9061 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 9121 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 9181 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 9241 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 9301 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 9361 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 9421 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 9481 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 9541 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 9601 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 9661 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 9721 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 9781 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 9841 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 9901 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 9961 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 10021 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 10081 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 10141 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 10201 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 10261 acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.