pLV3-CMV-ATF3-3×FLAG-CopGFP-Pu

pLV3-CMV-ATF3-3×FLAG-CopGFP-Puro plasmid

PVT96625

pLV3-CMV-ATF3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ATF3(human)-3×FLAG-CopGFP-Puro
Alias: NM_001674.4
Gene lenght: 543bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ATF3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-ATF3-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ATF3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga tgcttcaaca cccaggccag 2341 gtctctgcct cggaagtgag tgcttctgcc atcgtcccct gcctgtcccc tcctgggtca 2401 ctggtgtttg aggattttgc taacctgacg ccctttgtca aggaagagct gaggtttgcc 2461 atccagaaca agcacctctg ccaccggatg tcctctgcgc tggaatcagt cactgtcagc 2521 gacagacccc tcggggtgtc catcacaaaa gccgaggtag cccctgaaga agatgaaagg 2581 aaaaagaggc gacgagaaag aaataagatt gcagctgcaa agtgccgaaa caagaagaag 2641 gagaagacgg agtgcctgca gaaagagtcg gagaagctgg aaagtgtgaa tgctgaactg 2701 aaggctcaga ttgaggagct caagaacgag aagcagcatt tgatatacat gctcaacctt 2761 catcggccca cgtgtattgt ccgggctcag aatgggagga ctccagaaga tgagagaaac 2821 ctctttatcc aacagataaa agaaggaaca ttgcagagcg gatccGACTA CAAAGACCAT 2881 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg 2941 cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 3001 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 3061 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 3121 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 3181 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 3241 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 3301 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 3361 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 3421 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 3481 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc 3541 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag 3601 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc 3661 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac 3721 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc 3781 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag 3841 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg 3901 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc 3961 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac 4021 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag 4081 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg 4141 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct 4201 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag 4261 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag 4321 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 4381 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 4441 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 4501 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 4561 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 4621 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 4681 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 4741 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 4801 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 4861 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct 4921 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 4981 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 5041 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 5101 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 5161 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 5221 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 5281 gacaattccg tggtgttgtc ggggaaATCA TCGTCCTTTC CTtggctgct cgcctgtgtt 5341 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 5401 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct Tcgccttcgc 5461 cctcagacga gtcggatctc cctttggGcc gcctccccgc ctggtacctt taagaccaat 5521 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 5581 gctaattcac tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt 5641 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 5701 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 5761 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 5821 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 5881 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 5941 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 6001 tcatgtctgg ctctagctaT CCCGCCCCTA ACTCCGCCCA tcccgcccct aactccgccc 6061 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 6121 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 6181 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc 6241 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 6301 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 6361 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 6421 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6481 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 6541 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 6601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 6661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 6721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 6841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 6901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 6961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 7021 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 7081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7141 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 7201 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 7261 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 7321 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 7381 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 7441 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 7501 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 7561 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 7621 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 7681 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 7741 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 7801 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 7861 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 7921 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 7981 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 8041 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 8101 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 8161 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 8221 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 8281 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 8341 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 8401 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 8461 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 8521 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 8581 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 8641 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 8701 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 8761 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 8821 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.